Study of Mycoprotein Production Using Fusarium oxysporum PTCC 5115 and Reduction of its RNA Content
DOI:
https://doi.org/10.3923/pjn.2008.240.243Keywords:
Fusarium oxysporum, mycoprotein, RNA reductionAbstract
In this research, production of mycoprotein by Fusarium oxysporum (PTCC 5115) was investigated. In order to achieve the highest production yield, cultivation parameters including temperature, agitation speed, carbon and nitrogen sources were optimized by applying the Taguchi design. The optimum conditions obtained for mycoprotein production were: 25 &derg;C, 150 rpm, 5 g of glucose per liter and 3.4 g of ammonium dihydrogen phosphate per liter which resulted in the product containing 42% (w/w) crude protein. RNA content was reduced to an acceptable level through heat treatment of fungal biomass at 65&derg;C for 15 min. This heat shock procedure reduced RNA level to less than 1%, which is recognized as an acceptable level for human food grade products.
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