The Effect of the Water Additive KimchiStock® as an Herbal Growth Promoter on the Jejunal Histological and Ultrastructural Changes of Broiler Chickens
DOI:
https://doi.org/10.3923/pjn.2019.983.988Keywords:
Broiler, herbal growth promoter, jejunal villi, ultrastructure, water additiveAbstract
Background and Objective: There has been a great deal of attention on the use of herbal feed additives for replacing the utility of antimicrobial agents. The objective of the present study was to investigate the effect of adding KimchiStock® to drinking water on the histological and ultrastructural changes of the villi in the jejunum of broiler chickens. Materials and Methods: Fifty one-day-old broiler chickens, strain Cobb, were used as experimental animals. Chickens were randomly allotted into control (K1) and treated (K2) groups. K2 received a 0.2% water additive (KimchiStock®) in their drinking water for 5 days consecutively every week for 5 weeks. At the end of the treatment periods, the chicks in each group were killed by cervical dislocation and necropsied and jejunal samples were collected for histological and ultrastructural analyses. Results: Based on the description of the jejunal histopathology, the best results were found in the treated group. In the ultrastructural study, although there were no significant differences (p>0.05) between treatments for villi width; the height and the density of the jejunal villi in the treated (K2) group were significantly (p<0.05) higher and more dense compared to those in the control (K1) group. Conclusion: KimchiStock® increased immunomodulation through hyperplasia of lymphocytic cells, which allows it to prevent infection in poultry. KimchiStock® increases the height and density of the jejunal villi which helps maximize digestion capacity and growth performance. Thus, KimchiStock® can be used as a substitute for growth promoter antibiotics.
References
Furtula, V., E.G. Farrell, F. Diarrassouba, H. Rempel, J. Pritchard and M.S. Diarra, 2010. Veterinary pharmaceuticals and antibiotic resistance of Escherichia coli isolates in poultry litter from commercial farms and controlled feeding trials. Poult. Sci., 89: 180-188.
Merchant, L.E., H. Rempel, T. Forge, T. Kannangara and S. Bittman et al., 2012. Characterization of antibiotic-resistant and potentially pathogenic Escherichia coli from soil fertilized with litter of broiler chickens fed antimicrobial-supplemented diets Can. J. Microbiol., 58: 1084-1098.
Castanon, J.I.R., 2007. History of the use of antibiotic as growth promoters in European poultry feeds. Poult. Sci., 86: 2466-2471.
Smith, D.L., A.D. Harris, J.A. Johnson, E.K. Silbergeld and J.G. Morris Jr., 2002. Animal antibiotic use has an early but important impact on the emergence of antibiotic resistance in human commensal bacteria. Proc. Nat. Acad. Sci. USA., 99: 6434-6439.
Jaiswal, S.K., A.K. Chaturvedani, M. Raza, L. Dilliwar, K. Dhruw and V. Sahu, 2017. Review: Natural growth promoters, alternative to Antibiotic growth promoters on poultry. Int. J. Sci. Environ. Technol., 6: 254 -259.
Khan, I. and S.C. Kang, 2016. Probiotic potential of nutritionally improved Lactobacillus plantarum DGK-17 isolated from Kimchi-A traditional Korean fermented food. Food Control, 60: 88-94.
Yoon, J.H., S.S. Kang, T.I. Mheen, J.S. Ahn and H.J. Lee et al., 2000. Lactobacillus kimchii sp. nov., a new species from kimchi. Int. J. Syst. Evol. Microbiol., 50: 1789-1795.
Park, K.Y., J.H. Kil, K.O. Jung, C.S. Kong and L.M. Lee, 2006. Functional properties of Kimchi (Korean fermented vegetables). Acta Hortic., 706: 167-172.
Awad, W., K. Ghareeb and J. Bohm, 2008. Intestinal structure and function of broiler chickens on diets supplemented with a Synbiotic containing Enterococcus faecium and Oligosaccharides. Int. J. Mol. Sci., 9: 2205-2216.
Thanh, N.T., T.C. Loh, H.L. Foo, M. Hair-Bejo and B.K. Azhar, 2009. Effects of feeding metabolite combinations produced by Lactobacillus plantarum on growth performance, faecal microbial population, small intestine villus height and faecal volatile fatty acids in broilers. Br. Poult. Sci., 50: 298-306.
Thu, T.V., T.C. Loh, H.L. Foo, H. Yaakub and M.H. Bejo, 2011. Effects of liquid metabolite combinations produced by Lactobacillus plantarum on growth performance, faeces characteristics, intestinal morphology and diarrhoea incidence in postweaning piglets. Trop. Anim. Health Prod., 43: 69-75.
Vamanu, E., 2017. Effect of gastric and small intestinal digestion on lactic acid bacteria activity in a GIS1 simulator. Saudi J. Biol. Sci., 24: 1453-1457.
Steel, R.G.D. and J.H. Torrie, 1980. Principles and Procedures of Statistics: A Biometrical Approach. 2nd Edn., McGraw Hill, New York, United States, ISBN-13: 9780070609266, Pages: 633.
Goldstein, J., D.E. Newbury, P. Echlin, D.C. Joy and A.D. Romig Jr. et al., 2012. Scanning Electron Microscopy and X-Ray Microanalysis, A Text for Biologists, Materials Scientists and Geologists. 2nd Edn., Springer, New York, USA., Pages: 840.
Han, E.S., H.J. Kim and H.K. Choi, 2015. Health Benefits of Kimchi. In: Health Benefits of Fermented Foods, Tamang, J.P. (Ed.)., CRC Press, New York, USA., pp: 343-370.
Park, K.Y., J.K. Jeong, Y.E. Lee and J.W. Daily, 2014. Health benefits of kimchi (Korean fermented vegetables) as a probiotic food. J. Med. Food, 17: 6-20.
Chichlowski, M., W.J. Croom, F.W. Edens, B.W. McBride and R. Qiu et al., 2007. Microarchitecture and spatial relationship between bacteria and ileal, cecal and colonic epithelium in chicks fed a direct-fed microbial, PrimaLac and salinomycin. Poult. Sci., 86: 1121-1132.
Uni, Z., 2006. Early Development of Small Intestinal Function. In: Avian Gut Function in Health and Disease. Perry, G.C. (Ed.). CABI Publishing, Cambridge, MA., USA., ISBN-13: 9781845931803.
Rahimi, S., J.L. Grimes, O. Fletcher, E. Oviedo and B.W. Sheldon, 2009. Effect of a direct-fed microbial (Primalac) on structure and ultrastructure of small intestine in turkey poults. Poult. Sci., 88: 491-503.
Mountzouris, K.C., P. Tsirtsikos, E. Kalamara, S. Nitsch, G. Schatzmayr and K. Fegeros, 2007. Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poult. Sci., 86: 309-317.
Ariyadi, B. and S. Harimurti, 2015. Effect of indigenous probiotics lactic acid bacteria on the small intestinal histology structure and the expression of mucins in the ileum of broiler chickens. Int. J. Poult. Sci., 14: 276-278.
Sato, K., K. Takahashi, M. Tohno, Y. Miura, T. Kamada, S. Ikegami and H. Kitazawa, 2009. Immunomodulation in gut-associated lymphoid tissue of neonatal chicks by immunobiotic diets. Poult. Sci., 88: 2532-2538.
Jiang, J., B. Shi, D.Q. Zhu, Q.X. Cai and Y.R. Chen et al., 2012. Characterization of a novel bacteriocin produced by Lactobacillus sakei LSJ618 isolated from traditional Chinese fermented radish. Food Control, 23: 338-344.
Grosu-Tudor, S.S. and M. Zamfir, 2013. Functional properties of lactic acid bacteria isolated from Romanian fermented vegetables. Food Bacteriol., 27: 235-248.
Lee, J.K., D.W. Jung, Y.J. Kim, S.K. Cha, M.K. Lee and B.H. Ahn, 2009. Growth inhibitory effect of fermented kimchi on food-borne pathogens. Food Sci. Biotechnol., 18: 12-17.
Gaggia, F., D. Di Gioia, L. Buffoni and B. Biavati, 2011. The role of protective and probiotic cultures in food and feed and their impact in food safety. Trends Food Sci. Technol., 22: S58-S66.
Smalling, S., S.S. Diarra and F. Amosa, 2019. Effect of feed form and water addition on growth performance of finishing broilers in a hot humid environment. Pak. J. Nutr., 18: 339-345.
Susiani, R.D., R. Wasito and H. Wuryastuti, 2019. The effect of water additive commercial (KimchiStoc®) on natural avian influenza virus infection of broiler chickens: Pathological and immunopathological approach. East Afr. Schol. J. Agric. Life Sci., 2: 155-162.
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