Semen Quality, Fertility and Testicular Histology of Rabbit Bucks Orally Administrated with Ethanolic Grape Seed Extract
DOI:
https://doi.org/10.3923/pjn.2019.1084.1093Keywords:
Fertility, grape seed extract, rabbit, semen quality, testosterone hormoneAbstract
Objective: Effect of daily oral administration with ethanolic grape seed extract (EGSE) as enhancing factors on reproductive performance of APRI line rabbit bucks was investigated in this study. Materials and Methods: Total of 18 rabbit bucks (6 in each group) were treated with 3 mL distilled water containing 0 as control group, 75 and 125 mg kg–1 live body weight (LBW) from EGSE, for 28 days as a treatment period. Semen was collected twice a week for 8 wks as a collection period, then the average of physical semen characteristics were calculated for three semen collection intervals. Aspartate (AST) and alanine (ALT) transaminases activity, total antioxidant capacity (TAC) and testosterone concentrations were determined in blood serum. Results: Administrated with 125 mg EGSE kg–1 LBW showed a significant improved (p<0.05) testicular weights, semen quality (progressive motility, livability, abnormality, acrosomal damage, cell concentration and outputs/ejaculate of spermatozoa), except semen volume and by advancing semen collection interval, testosterone and TAC concentrations, while, ALT activity (p<0.05) reduced compared with the control group. Conclusion: Practically, using natural antioxidant such as grape seed extract for 28 days, may increase the antioxidative capacity of testicular tissues of rabbit bucks to improve semen quality and reproductive traits.
References
Ma, Z.F. and H. Zhang, 2017. Phytochemical constituents, health benefits and industrial applications of grape seeds: A mini-review. Antioxidants, Vol. 6, No. 3.
El-Ashmawy, I.M., A. Saleh and O.M. Salama, 2007. Effects of marjoram volatile oil and grape seed extract on ethanol toxicity in male rats. Basic Clin. Pharmacol. Toxicol., 101: 320-327.
Garavaglia, J., M.M. Markoski, A. Oliveira and A. Marcadenti, 2016. Grape seed oil compounds: Biological and chemical actions for health. Nutr. Metab. Insights, 9: 59-64.
Dulundu, E., Y. Ozel, U. Topaloglu, H. Toklu, F. Ercan, N. Gedik and G. Sener, 2007. Grape seed extract reduces oxidative stress and fibrosis in experimental biliary obstruction. J. Gastroenterol. Hepatol., 22: 885-892.
MartÃnez-Flórez, S., J. González-Gallego, J.M. Culebras and M.J. Tuñón, 2002. Flavonoids: Properties and anti-oxidizing action. Nutr. Hosp., 17: 271-278, (In Spanish).
Maier, T., A. Schieber, D.R. Kammerer and R. Carle, 2009. Residues of grape (Vitis vinifera L.) seed oil production as a valuable source of phenolic antioxidants. Food Chem., 112: 551-559.
Shi, J., J. Yu, J.E. Pohorly and Y. Kakuda, 2003. Polyphenolics in grape seeds-biochemistry and functionality. J. Med. Food, 6: 291-299.
Singh, R.P., A.K. Tyagi, S. Dhanalakshmi, R. Agarwal and C. Agarwal, 2004. Grape seed extract inhibits advanced human prostate tumor growth and angiogenesis and upregulates insulinâ€like growth factor binding proteinâ€3. Int. J. Cancer, 108: 733-740.
Yilmaz, Y. and R.T. Toledo, 2004. Health aspects of functional grape seed constituents. Trends Food Sci. Technol., 15: 422-433.
Shenoy, S.F., C.L. Keen, S. Kalgaonkar and J.A. Polagruto, 2007. Effects of grape seed extract consumption on platelet function in postmenopausal women. Thromb. Res., 121: 431-432.
De Lamirande, E. and C. Gagnon, 1992. Reactive oxygen species and human spermatozoa. II. Depletion of adenosine triphosphate plays an important role in the inhibition of sperm motility. J. Androl., 13: 379-386.
Aitken, R.J., J.S. Clarkson and S. Fishel, 1989. Generation of reactive oxygen species, lipid peroxidation and human sperm function. Biol. Reprod., 41: 183-187.
Wishart, G.J., 1984. Effects of lipid peroxide formation in fowl semen on sperm motility, ATP content and fertilizing ability. J. Reprod. Fertil., 71: 113-118.
Roberts, K.P., 1998. Y-chromosome deletions and male infertility: State of the art and clinical implications. J. Androl., 19: 255-259.
Theau-Clément, M., 2007. Preparation of the rabbit doe to insemination: A review. World Rabbit Sci., 15: 61-80.
Brun, J.M., M. Theau-Clement and G. Bolet, 2002. The relationship between rabbit semen characteristics and reproductive performance after artificial insemination. Anim. Reprod. Sci., 70: 139-149.
Nizza, A., C. Di-Meo and S. Taranto, 2000. Influence of dietary protein content on libido and semen characteristics bucks. Proceedings of 7th World Rabbit Congress, July 2000, Valencia, Spain, pp: 217-224.
Castellini, C., P. Lattaioli, A. Dal Bosco, A. Minelli and C. Mugnai, 2003. Oxidative status and semen characteristics of rabbit buck as affected by dietary vitamin E, C and n-3 fatty acids. Reprod. Nutr. Dev., 43: 91-103.
Yousef, M.I., G.A. Abdallah and K.I. Kamel, 2003. Effect of ascorbic acid and Vitamin E supplementation on semen quality and biochemical parameters of male rabbits. Anim. Reprod. Sci., 76: 99-111.
Baker, H.W.G., J. Brindle, D.S. Irvine and R.J. Aitken, 1996. Protective effect of antioxidants on the impairment of sperm motility by activated polymorphonuclear leukocytes Fertil. Steril., 65: 411-419.
Abou Khadiga, G., Y.M.K. Youssef, K. Saleh, R.Y. Nofal and M. Baselga, 2010. Genetic trend in selection for litter weight in two maternal lines of rabbits in Egypt. World Rabbit Sci., 18: 27-32.
Ewuola, E.O. and G.N. Egbunike, 2010. Effects of dietary fumonisin B1 on the onset of puberty, semen quality, fertility rates and testicular morphology in male rabbits Reproduction, 139: 439-445.
Blom, E., 1983. Sperm morphology with reference to bull infertility. Proceedings of the 1st All-India Symposium on Animal Reproduction, (AR'83), Ludhiana, India, pp: 61-81.
El-Ratel, I.T., 2017. Impact of lycopene or folic acid treatment on semen quality, blood constituents and fertility of rabbit bucks. Egypt. J. Nutr. Feeds, 20: 213-223.
Lomeo, A.M. and A.M. Giambersio, 1991. ‘Water-test’: A simple method to assess sperm-membrane integrity. Int. J. Androl., 14: 278-282.
Reitman, S. and S. Frankel, 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol., 28: 56-63.
Bancroft, D. and A. Stevens, 1982. Theory and Practice of Histological Techniques. 2nd Edn., Churchill Livingstone, Edinburgh, London, Melaborne, Pages: 262.
SAS., 2004. SAS user’s guide, statistical. Version 9.1, SAS Institute Inc., Cary, NC, USA.
Duncan, D.B., 1955. Multiple range and multiple F tests. Biometrics, 11: 1-42.
Best, P., 2006. Warning against rapeseed oil for pigs. Feed International, September 11-13, 2006
Goni, I., A. Brenes, C. Centeno, A. Viveros and F. Saura-Calixto et al., 2007. Effect of dietary grape pomace and vitamin E on growth performance, nutrient digestibility and susceptibility to meat lipid oxidation in chickens. Poult. Sci., 86: 508-516.
Fiesel, A., D.K. Gessner, E. Most and K. Eder, 2014. Effects of dietary polyphenol-rich plant products from grape or hop on pro-inflammatory gene expression in the intestine, nutrient digestibility and faecal microbiota of weaned pigs. BMC Vet. Res., Vol. 10.
Viveros, A., S. Chamorro, M. Pizarro, I. Arija, C. Centeno and A. Brenes, 2011. Effects of dietary polyphenol-rich grape products on intestinal microflora and gut morphology in broiler chicks. Poult. Sci., 90: 566-578.
Hafsa, S.H.A., H.A. Ayman, A.S. Elham, A. Popescu and D. Mocuta, 2016. Grape seed (Vitis vinifera L.) alleviate reproductive toxicity caused by lindane in New Zeeland white male rabbits. Sci. Pap. Ser. Manage. Econ. Eng. Agric. Rural Dev., 16: 17-28.
Abdel-Khalek, A.E., S.A. Darwish, A.F. Mehrez and M.B. Aboul-Ela, 2001. Morpho-histmetric study on testis, epididymis and ampulla of fertile and sub-fertile Egyptian buffaloes. J. Agric. Sci. Mansoura Univ., 8: 4773-4786.
Awoniyi, D.O., 2010. The role of rooibos (Aspalathus Linearis), green tea (Camellia sinensis) and commercially available rooibos and green tea antioxidant supplements on rat testicular and epididymal function. M.Sc. Thesis, Faculty of Health and Wellness Science, Cape Peninsula University of Technology, South Africa.
Yildirim, N.C., F.M. Kandemir and F. Benzer, 2011. Beneficial effects of grape seed extract against Cisplatin-induced testiculer damage in rabbits. Digest J. Nanomat. Biostruct., 6: 155-159.
Chis, I.C., M.I. Ungureanu, A. Marton, R. Simedrea, A. Muresan, I.D. Postescu and N. Decea, 2009. Antioxidant effects of a grape seed extract in a rat model of diabetes mellitus. Diab. Vasc. Dis. Res., 6: 200-204.
Pari, L. and A. Suresh, 2008. Effect of grape (Vitis vinifera L.) leaf extract on alcohol induced oxidative stress in rats. Food Chem. Toxicol., 46: 1627-1634.
Monagas, M., B. Hernandez-Ledesma, C. Gomez-Cordoves and B. Bartalome, 2006. Commercial dietary ingredients from Vitis vinifera L. leaves and grape skins: Antioxidant and chemical characterization. J. Agric. Food Chem., 54: 319-327.
Murthy, K.N.C., R.P. Singh and G.K. Jayaprakasha, 2002. Antioxidant activities of grape (Vitis vinifera) pomace extracts. J. Agric. Food Chem., 50: 5909-5914.
Marin-Guzman, J., D.C. Mahan and J.L. Pate, 2000. Effect of dietary selenium and vitamin E on spermatogenic development in boars. J. Anim. Sci., 78: 1537-1543.
Eid, Y., T. Ebeid and H. Younis, 2006. Vitamin E supplementation reduces dexamethasone-induced oxidative stress in chicken semen. Br. Poult. Sci., 47: 350-356.
Halliwell, B., 1996. Antioxidants in human health and disease. Annu. Rev. Nutr., 16: 33-50.
Rice-Evans, C. and L. Packer, 1998. Flavonoids in Health and Disease. Marcel Dekker, New York, USA., ISBN: 9780824700966, Pages: 541.
Sallam, S.M.A., M.E.A. Nasser, M.S.H. Yousef, A.M. El-Morsy, S.A.S. Mahmoud and M.I. Yousef, 2005. Influence of aluminum chloride and ascorbic acid on performance, digestibility, caecal microbial activity and biochemical parameters of rabbits. Res. J. Agric. Biol. Sci., 1: 10-16.
Downloads
Published
Issue
Section
License
Copyright (c) 2019 The Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
