Mechanism of Anti-Angiogenic and Renal Protective Activity of Balanites aegyptiaca Seeds Extract in Ehrlich Ascites Carcinoma-Bearing Mice
Authors
- Ahmed A. Emara Department of Radiology and Medical Imaging, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
- Maraym F. Dawood Department of Radiology and Medical Imaging, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
- Wafaa A. Abdallah Department of Radiology and Medical Imaging, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
- Aya Z. Hazaa Department of Radiology and Medical Imaging, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
- Alyaa A. Ahmed Department of Radiology and Medical Imaging, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
- Aya S. Elbayoumy Department of Radiology and Medical Imaging, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
- Asmaa M. El-nossairy Department of Radiology and Medical Imaging, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
- Nuran S. Yaqoub Department of Radiology and Medical Imaging, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
- Shymaa H. Khalifa Department of Radiology and Medical Imaging, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
- Elshimaa O. Abdelaziz Department of Radiology and Medical Imaging, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
- Abdelrahman B. Khairy Department of Radiology and Medical Imaging, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
- Naglaa A. Gobba Department of Pharmacology, Faculty of Pharmacy, MUST University, October 6th City, Egypt
- Esraa A. Abdalla Department of Biochemistry, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
- Ismail Hegazy Department of Biochemistry, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
-
Mohammed A. Hussein
Department of Biochemistry, Faculty of Applied Medical Science, October 6th University, October 6th City, Egypt
DOI:
https://doi.org/10.3923/pjn.2021.46.54Keywords:
Anticancer, balanites aegyptiaca, ehrlich ascites carcinoma, mice, renal carcinoma cell lineAbstract
Background: One of the animal angiogenesis with high inflammation and rapid growth is Ehrlich ascites carcinoma. Balanites aegyptiaca seeds extract (BASE), a new synthesized compound has antioxidant and antidiabetic activity. The purpose of this study was to evaluate anti-angiogenic activity of BASE in ehrlich ascites carcinoma (EAC)-bearing mice. Materials and Methods: BASE was prepared and characterized using instrumental analysis and spectral data. Furthermore, the IC50 of BASE against the renal carcinoma cell line (RCC-949) was calculated. Adult albino mice weighing 25±5 g was used to assess the anti-angiogenic activity of BASE (100 and 200 mg kg‾1 body weight) in EAC-bearing mice. Results: IC50 of BASE against the renal carcinoma cell line (RCC-949) was equal to 62.18 µg mL‾1. The daily oral administration of BASE at concentrations of 100 and 200 mg kg‾1 body weight for 30 days to EAC-bearing mice resulted in a significant improvement in tumor volume and tumor weight, urea, creatinine, uric acid, TNF-α, NOx, TBARs, GSH, CAT, SOD, GPx and VEGF-C gene expression in EAC-bearing mice. Furthermore, BASE almost normalized these effects in renal histoarchitecture. Conclusion: The BASE has anti-angiogenic activity in EAC-bearing mice.
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