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Mechanism of Anti-Angiogenic and Renal Protective Activity of Balanites aegyptiaca Seeds Extract in Ehrlich Ascites Carcinoma-Bearing Mice
Abstract
Background: One of the animal angiogenesis with high inflammation and rapid growth is Ehrlich ascites carcinoma. Balanites aegyptiaca seeds extract (BASE), a new synthesized compound has antioxidant and antidiabetic activity. The purpose of this study was to evaluate anti-angiogenic activity of BASE in ehrlich ascites carcinoma (EAC)-bearing mice. Materials and Methods: BASE was prepared and characterized using instrumental analysis and spectral data. Furthermore, the IC50 of BASE against the renal carcinoma cell line (RCC-949) was calculated. Adult albino mice weighing 25±5 g was used to assess the anti-angiogenic activity of BASE (100 and 200 mg kg‾1 body weight) in EAC-bearing mice. Results: IC50 of BASE against the renal carcinoma cell line (RCC-949) was equal to 62.18 µg mL‾1. The daily oral administration of BASE at concentrations of 100 and 200 mg kg‾1 body weight for 30 days to EAC-bearing mice resulted in a significant improvement in tumor volume and tumor weight, urea, creatinine, uric acid, TNF-α, NOx, TBARs, GSH, CAT, SOD, GPx and VEGF-C gene expression in EAC-bearing mice. Furthermore, BASE almost normalized these effects in renal histoarchitecture. Conclusion: The BASE has anti-angiogenic activity in EAC-bearing mice.
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Anticancer, balanites aegyptiaca, ehrlich ascites carcinoma, mice, renal carcinoma cell lineArticle Metrics
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Emara, A. A., Dawood, M. F., Abdallah, W. A., Hazaa, A. Z., Ahmed, A. A., Elbayoumy, A. S., El-nossairy, A. M., Yaqoub, N. S., Khalifa, S. H., Abdelaziz, E. O., Khairy, A. B., Gobba, N. A., Abdalla, E. A., Hegazy, I., & Hussein, M. A. (2021). Mechanism of Anti-Angiogenic and Renal Protective Activity of Balanites aegyptiaca Seeds Extract in Ehrlich Ascites Carcinoma-Bearing Mice. Pakistan Journal of Nutrition, 20(2), 46–54. https://doi.org/10.3923/pjn.2021.46.54
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