Research Article Open Access

Histomorphology of Testes in Honey Treated Adult Male Albino Rats

Authors

Anulika O. Onyemelukwe
Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Enugu Campus, Enugu, Nigeria
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Rita I-O. Ogu
Department of Human Physiology, Faculty of Basic Medical Sciences, University of Science and Technology, Enugu State, Nigeria
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Nkiruka C. Azubuike
Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Enugu Campus, Enugu, Nigeria
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CORRESPONDING AUTHOR
Cornelius O. Ogu
iD 0000-0002-1696-4336
Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Enugu Campus, Enugu, Nigeria
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Okechukwu S. Onwukwe
Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Enugu Campus, Enugu, Nigeria
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Udoh P. Iniekong
Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Enugu Campus, Enugu, Nigeria
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Basil C. Udeh
Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Enugu Campus, Enugu, Nigeria
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Peter U. Achukwu
Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Enugu Campus, Enugu, Nigeria
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Ikenna K. Uchendu
Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Enugu Campus, Enugu, Nigeria
Published on: July 13, 2023
DOI 10.3923/pjn.2023.88.94
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Abstract

Objective: Present study was conducted to examine the histomorphology of testes and serum testosterone levels of adult male albino rats fed with honey. Materials and Methods: The albino rats were divided into four groups of eight per cage. Controls received drinking water. Groups A, B and C were fed with 0.15, 0.3 and 0.6 mL kg–1 b.wt. of honey respectively twice a day, orally for four and eight weeks. Blood samples were obtained from 4 rats per group for testosterone estimation. Testes were excised for histomorphology. Results: After eight weeks, testes from control and honey-treated rats showed normal histoarchitecture, whereas four-week honey-treated rats showed decreased prominence of seminiferous tubules with prominent sertoli cells and reduction of leydig cells. After four weeks, honey-treated rats showed significant elevation in serum testosterone level (p<0.05), but after eight-week, no significant change (p>0.05) was observed, compared to controls. Conclusion: Honey consumption could have a short-term positive effect on testosterone secretion and may lead to hyperactive testicular cells.

Keywords:

Albino rats, honey, leydig cells, sertoli cells, testes, testicular tissues, testosterone

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This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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Onyemelukwe, A. O., Ogu, R. I.-O., Azubuike, N. C., Ogu, C. O., Onwukwe, O. S., Iniekong, U. P., Udeh, B. C., Achukwu, P. U., & Uchendu, I. K. (2023). Histomorphology of Testes in Honey Treated Adult Male Albino Rats. Pakistan Journal of Nutrition, 22, 88–94. https://doi.org/10.3923/pjn.2023.88.94
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