Research Article Open Access

Development and Characterization of a Culinary Bouillon Cube from Wild Edible Mushrooms in Benin

Authors

Mawutin Justine Bérénice Houssou-Goe
Food Health Safety Research Unit of Laboratory of Microbiology, Food Technology and Phytopathology, Department of Plant Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, Benin
|
CORRESPONDING AUTHOR
Comlan Kintomagnimessè Célestin Tchekessi
Food Health Safety Research Unit of Laboratory of Microbiology, Food Technology and Phytopathology, Department of Plant Biology, Faculty of Sciences and Technology, University of Abomey-Calavi, Benin
|
Dossa Appolinaire Kinsou Djossou
Food Health Safety Research Unit of Laboratory of Microbiology, Food Technology and Phytopathology, Department of Plant Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, Benin
|
G. Justin Gandeho
Laboratory of Nutrition and Food Sciences, Departement of Nutrition and Food Sciences, Faculty of Agronomy, Universy of Parakou, Bénin
|
G. Hospice Dassou
Laboratory of Plant Sciences and Pharmacopoeia, Department of Plant Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, Benin
|
D. Roël Houdanon
Laboratory of Ecology, Botany and Plant Biology, Faculty of Agronomy, University of Parakou, Benin
|
Déley Sylvain Dabade
Laboratory of Sciences and Food Technologies, Faculty of Agronomic Sciences, University of Abomey-Calavi, Benin
|
Marius Eric Badoussi
Laboratory of Biochemistry and Food and Medicinal Formulations, National Higher School of Biosciences and Applied Biotechnologies, National University of Sciences, Technologies, Engineering and Mathematics, Benin
|
Roseline Bleoussi
Food Health Safety Research Unit of Laboratory of Microbiology, Food Technology and Phytopathology, Department of Plant Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, Benin
|
Anayce Djogbe
Food Health Safety Research Unit of Laboratory of Microbiology, Food Technology and Phytopathology, Department of Plant Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, Benin
|
Innocent Bokossa Yaou
Food Health Safety Research Unit of Laboratory of Microbiology, Food Technology and Phytopathology, Department of Plant Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, Benin
|
Farid Abdel Kader Baba-Moussa
Food Health Safety Research Unit of Laboratory of Microbiology, Food Technology and Phytopathology, Department of Plant Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, Benin
DOI 10.66920/pjn.2026.74.84
PDF

Abstract

Background and Objective: The consumption of industrial culinary bouillons is widespread in West Africa, although their formulation often relies on high salt contents and the use of food additives. This study aimed to develop a culinary bouillon based on the local edible mushroom Volvariella volvacea as an alternative to industrial bouillons available in Benin.

Materials and Methods: The bouillon cube was formulated using edible mushroom powder (40%), natural spices (17.86%), salt (10.71%), starch (2.86%) and water (28.57%) and was subsequently subjected to physicochemical, microbiological and sensory evaluations.

Results: The production process yielded 81.17%. Physicochemical analyses showed moisture, salt, protein and ash contents of 6.41±0.02%, 4.83±0.096%, 19.38±0.17% and 2.15±0.013%, respectively. Microbiological analyses indicated satisfactory hygienic quality of both the mushroom powder and the finished bouillon, with the absence or low levels of the main microorganisms investigated. Sensory evaluation, conducted in comparison with a commercial bouillon, showed greater acceptance of the developed product in terms of texture, aroma, taste, aftertaste and overall acceptability, whereas its color received a slightly lower score.

Conclusion: Overall, the Volvariella volvacea-based culinary bouillon cube exhibited satisfactory physicochemical, microbiological and sensory characteristics and represents a promising approach for valorizing locally available edible mushrooms in Benin.

Keywords:

Culinary bouillon cube, Volvariella volvacea, edible mushrooms, quality, Benin

Article Metrics

0 Abstract Views
0 File Views
0 Downloads

Article Citation Metrics

How to Cite

Houssou-Goe, M. J. B., Tchekessi, C. K. C., Kinsou Djossou, D. A., Gandeho, G. J., Dassou, G. H., Houdanon, D. R., Dabade, D. S., Badoussi, M. E., Bleoussi, R., Djogbe, A., Bokossa Yaou, I., & Baba-Moussa, F. A. K. (2026). Development and Characterization of a Culinary Bouillon Cube from Wild Edible Mushrooms in Benin. Pakistan Journal of Nutrition, 25, 74–84. https://doi.org/10.66920/pjn.2026.74.84

References

1.
Archer, N.S., M. Cochet-Broch, M. Mihnea, G. Garrido-Bañuelos, P. Lopez-Sanchez, et al., 2022. Sodium reduction in bouillon: Targeting a food staple to reduce hypertension in Sub-Saharan Africa. Front. Nutr., Vol. 9. 10.3389/fnut.2022.746018
2.
Adams, K.P., S.A. Vosti, E. Becher, F. Ishaya and R. Engle‐Stone, 2024. Bouillon fortification as a strategy to address inequities in micronutrient adequacy of diets in Nigeria. Ann. N Y. Acad. Sci., 1540: 235-250.
3.
Dossou-Yovo, P., L.T.C. Tossou, A. Sezan and R.A.C. Yelouassi, 2016. Evaluation of the nutritional quality of the most consumed seasonings named «cube» in South of Benin. Int. J. Innovation Appl. Stud., 17: 94-99.
4.
Fregene, C., 2024. Sodium intake risk assessment of some bouillon cubes and adoption of the who sodium benchmark in Nigeria. Nutr. Food Sci. Int. J., Vol. 13. 10.19080/NFSIJ.2024.13.555864
5.
Yang, L., Y. Gao, J. Gong, L. Peng, H.R. El-Seedi, et al., 2023. A multifaceted review of monosodium glutamate effects on human health and its natural remedies. Food Mater. Res., Vol. 3. 10.48130/fmr-2023-0016
6.
Talata, O., F.Z. Moussa and F.M. Cissé, 2021. Les bouillons cubes (souvent appelés cubes Maggi), des condiments qui peuvent être remplacés (épisode 1). Réseau National des Chambres d’Agriculture du Niger (RECA-Niger). Pages: 8. https://reca-niger.org/IMG/pdf/bouillons_cube_note_5_mai_2021.pdf
7.
Wang, X., S. Ye, X. Yan, Q. Zhang, Q. Lu and H. Liang, 2025. Prospects and challenges of umami in edible fungi: Biological sources, structure‐function relationship, production, mechanism, and evaluation. Compr. Rev. s Food Sci. Food Saf., Vol. 25. 10.1111/1541-4337.70305
8.
Kesel, A.D., A.-A. Boukary, N.S. Yorou and J. Degreef, 2024. Champignons comestibles d'Afrique de l'Ouest. Royal Belgian Institute of Natural Sciences, Brussels, Belgium, ISBN: 978-9-0732-4277-7, ISBN 978-9-0732-4278-4.
9.
Bamba, L., 2025. Formulation et évaluation de la qualité sanitaire d'un bouillon alimentaire à base de grains fermentés de néré (Parkia biglobosa) en Côte d'Ivoire. Ph.D. Thesis. Université Jean Lorougnon Guédé, Côte d'Ivoire.
10.
AOAC International and G.W. Latimer Jr., 2016. Official Methods of Analysis of AOAC International. 20th ed., AOAC International, Rockville, Maryland, USA, ISBN: 0-935584-87-0.
11.
AOAC International, 2006. Official Methods of Analysis of AOAC International. 18th ed., AOAC International, Gaithersburg, Maryland, USA, ISBN: 0-935584-77-3, 978-0-935584-77-6.
12.
ISO, 2017. Microbiology of the food chain: Preparation of test samples, initial suspension and decimal dilutions for microbiological examination, Part 1: General rules for the preparation of the initial suspension and decimal dilutions. International Organization for Standardization. ISO 6887-1:2017. ISO/TC 34/SC 9. Geneva, Switzerland. Pages: 28. https://www.iso.org/fr/standard/63335.html
13.
Teramura, H., G. Betts, Y. Chen, M. Brodsky and Y. Salfinger, 2018. MC-Media pad ACplus™ for enumeration of aerobic counts in a variety of foods. J. AOAC. Int., 101: 769-782.
14.
Buyser, M.D., 2003. Validation of EN ISO standard methods 6888 Part 1 and Part 2: 1999—Enumeration of coagulase-positive staphylococci in foods. Int. J. Food Microbiol., 83: 185-194.
15.
ISO, 1998. Microbiologie des aliments — Méthode horizontale pour le dénombrement des bactéries lactiques mésophiles — Technique par comptage des colonies à 30 degrés C. ISO 15214:1998. 1st Edn. International Organization for Standardization. Geneva, Switzerland. ISO/TC 34/SC 9. 07.100.30. Pages: 7. https://www.iso.org/fr/standard/26853.html
16.
Beuchat, L.R., E. Frändberg, T. Deak, S.M. Alzamora, J. Chen, et al., 2001. Performance of mycological media in enumerating desiccated food spoilage yeasts: An interlaboratory study. Int. J. Food Microbiol., 70: 89-96.
17.
ISO, 2006. Microbiologie des aliments — Méthode horizontale pour le dénombrement des coliformes — Méthode par comptage des colonies. ISO 4832:2006. International Organization for Standardization. 3rd Edn. Geneva, Switzerland. ISO/TC 34/SC 9. 07.100.30. Pages: 6. https://www.iso.org/fr/standard/38282.html
18.
Teramura, H., A. Ogura, L. Everis and G. Betts, 2019. MC-media pad EC for enumeration of Escherichia coli and coliforms in a variety of foods: AOAC performance tested MethodSM 011902. J. AOAC. Int., 102: 1502-1515.
19.
ISO, 2006. Microbiologie des aliments — Méthode horizontale pour le dénombrement des coliformes — Méthode par comptage des colonies. ISO 4832:2006. 3rd Edn. ISO/TC 34/SC 9. Geneva, Switzerland. 07.100.30. Pages: 6. https://www.iso.org/fr/standard/38282.html
20.
ASM, 2019. Gram Stain Protocols. American Society for Microbiology. Washington, DC, USA. https://asm.org/protocols/gram-stain-protocols
21.
Civille, G.V., B.T. Carr and K.E. Osdoba, 2026. Sensory Evaluation Techniques. 6th ed., CRC Press, Boca Raton, Florida, USA, ISBN: 9781032399416, 1032399414. Pages: 563.
22.
ASN, 2018. Bouillons alimentaires d’assaisonnement - Spécifications. NS 03-146. Association Sénégalaise de Normalisation. Dakar, Senegal. Pages: 2. https://asn.sn/fr/node/1076/pdf
23.
CAC, 1981. Norme Codex pour les champignons comestibles séchés. FAO and WHO. CODEX STAN 39-1981. CAC/RS 39-1970. Codex Alimentarius Commission. Rome, Italy. Pages: 4. https://www.fao.org/input/download/standards/232/CXS_039f.pdf
24.
Sahoré, A.D., J.G. Nemlin and A.F. Tetchi, 2012. Study of physicochemical properties of some traditional vegetables in Ivory Coast: Seeds of Beilschmiedia mannii (Lauraceae), seeds of Irvingia gabonensis (Irvingiaceae) and Volvariella volvaceae. Food Nutr. Sci., 3: 14-17.
25.
Fellows, P.J, 2017. Food Processing Technology: Principles and Practice. 4th ed., Woodhead Publishing, Duxford, United Kingdom, ISBN: 9780081019078, 0081019076. Pages:1152.
26.
Kouame, K.B., A.C. Koko, M. Diomande, I. Konate and N.E. Assidjo, 2018. Caractérisation physicochimique de trois espèces de champignons sauvages comestibles couramment rencontrées dans la région du haut-sassandra (côte d’ivoire). J. Appl. Biosci., 121: 12110-12120.
27.
Cheung, P.C.K., 2010. The nutritional and health benefits of mushrooms. Nutr. Bull., 35: 292-299.
28.
WHO, 2012. Guideline: Sodium Intake for Adults and Children. World Health Organization, Geneva, Switzerland, ISBN: 978-92-4-150483-6, 9241504838. Pages: 56.

Most read articles by the same author(s)