Antioxidative Activities of Aqueous and Ethanolic Extracts of Licorice Roots
DOI:
https://doi.org/10.3923/pjn.2014.267.270Keywords:
Antioxidants, aqueous and ethanolic, extract, licorice rootsAbstract
The licorice roots, is known as a healthy nutrient for more than 3000 years. The antioxidant activities of licorice roots, aqueous and ethanolic extracts, have been studied by using two different methods (reducing power and chelating ability). It was found that the total phenolic compounds in aqueous and ethanolic extracts of licorice roots were 4.8 and 9.2 mg/100 mg dry extract, respectively. The flavonoids (which are a part of the phenolic compounds) were found to be 2.3 and 6.8 mg/100 mg dry extract in aqueous and ethanolic extract of licorice roots respectively. The ethanolic extract shows high antioxidative activity as compared with aqueous extract. The aqueous and ethanolic extracts of licorice roots show high reducing power ability comparing with their abilities as chelating agents. Thus, this study suggests that licorice extract can be used as a potential source of natural antioxidants.
References
Arystanova, T., M. Irismetov and A. Sopbekova, 2001. Chromatographic determination of glycyrrhizinic acid in Glycyrrhiza glabra preparation. Chem. Nat. Compd., 37: 89-91.
Asl, M.N. and H. Hosseinzadeh, 2008. Review of pharmacological effects of Glycyrrhiza sp. and its bioactive compounds. Phytother. Res., 22: 709-724.
Ayoola, G.A., S.S. Ipav, M.O. Sofidiya, A.A. Adepoju-Bello, H. Coker and T. Odugbemi, 2008. Phytochemical screening and free radical scavenging activities of the fruits and leaves of Allanblackia floribunda Oliv. (Guttiferae). Int. J. Health Res., 1: 87-93.
Biondi, D.M., C. Rocco and G. Ruberto, 2005. Dihydrostilbene derivatives from Glycyrrhiza glabra leaves. J. Nat. Prod., 68: 1099-1102.
Chou, H.J., J.T. Kuo and E.S. Lin, 2009. Comparative antioxidant properties of water extracts from different parts of beefsteak plant (Perilla frutescens). J. Food Drug Anal., 17: 489-496.
Chung, Y.C., C.T. Chang, W.W. Chao, C.F. Lin and S.T. Chou, 2002. Antioxidative activity and safety of the 50 ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1. J. Agric. Food Chem., 50: 2454-2458.
Di Mambro, V.M. and M.J.V. Fonseca, 2005. Assays of physical stability and antioxidant activity of a topical formulation added with different plant extract. J. Pharmaceut. Biomed. Anal., 37: 287-295.
Ebrahimzadeh, M.A., S.F. Nabavi, S.M. Nabavi and F. Pourmorad, 2010. Nitric oxide radical scavenging potential of some Elburz medicinal plants. Afr. J. Biotechnol., 9: 5212-5217.
Elmastas, M., I. Gulcin, O. Isildak, O.I. Kufrevioglu, K. Ibaoglu and H.Y. Aboul-Enein, 2006. Radical scavenging activity and antioxidant capacity of bay leaf extracts. J. Iran. Chem. Soc., 3: 258-266.
Elmastas, M., I. Gulcin, L. Ozturk and I. Gokce, 2005. Investigation of antioxidant properties of spearmint (Mentha spicata L.). Asian J. Chem., 17: 137-148.
Elmastas, M., I. Turkekul, L. Ozturk, I. Gulcin, O. Isildak and H.Y. Aboul-Enein, 2006. Antioxidant activity of two wild edible mushrooms (Morchella vulgaris and Morchella esculanta) from North Turkey. Combinatorial Chem. High Throughput Screening, 9: 443-448.
Fukai, T., B. Cai, K. Maruno, Y. Miyakawa, M. Konishi and T. Nomura, 1998. An isoprenylated flavanone from Glycyrrhiza glabra and rec-assay of licorice phenols. Phytochemistry, 49: 2005-2013.
Gulcin, I., M.E. Buyukokuroglu, M. Oktay and O.I. Kufrevioglu, 2002. On the in vitro antioxidative properties of melatonin. J. Pineal Res., 33: 167-171.
Gulcin, I., M.E. Buyukokuroglu, M. Oktay and O.I. Kufrevioglu, 2003. Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. subsp. pallsiana (Lamb.) Holmboe. J. Ethnopharmacol., 86: 51-58.
Gulcin, I., R. Elias, A. Gepdiremen, K. Taoubi and E. Koksal, 2009. Antioxidant secoiridoids from fringe tree (Chionanthus virgInicus L.). Wood Sci. Technol., 43: 195-212.
Gulcin, I., M. Elmastas and H.Y. Aboul-Enein, 2007. Determination of antioxidant and radical scavenging activity of basil (Ocimum basilicum L. Family Lamiaceae) assayed by different methodologies. Phytother. Res., 21: 354-361.
Gülçin, İ., V. Mshvildadze, A. Gepdiremen and R. Elias, 2006. The antioxidant activity of a triterpenoid glycoside isolated from the berries of Hedera colchica: 3-O-(β-D-glucopyranosyl)-hederagenin. Phytother. Res., 20: 130-134.
Gulcin, I., M. Oktay, O.I. Kufrevioglu and A. Aslan, 2002. Determination of antioxidant activity of lichen Cetraria islandica (L.) Ach. J. Ethnopharmacol., 79: 325-329.
Hseu, Y.C., W.H. Chang, C.S. Chen, J.W. Liao and C.J. Huang et al., 2008. Antioxidant activities of Toona sinensis leaves extracts using different antioxidant models. Food Chem. Toxicol., 46: 105-114.
Inchuen, S., W. Narkrugsa and P. Pornchaloempong, 2010. Effect of drying methods on chemical composition, color and antioxidant properties of Thai red curry powder. Kasetsart J. Nat. Sci., 44: 142-151.
Nabavi, S.M., M.A. Ebrahimzadeh, S.F. Nabavi, M. Fazelian and B. Eslami, 2009. In vitro antioxidant and free radical scavenging activity of Diospyros lotus and Pyrus boissieriana growing in Iran. Pharmacogn. Mag., 4: 122-126.
Nassiri-Asl, M., Saroukhani and F. Zamansoltani, 2007. Anticonvulsant effects of aqueous extract of Glycyrrhiza glabra root in PTZ-induced seizure in mice. Int. J. Pharmacol., 3: 432-434.
Oktay, M., İ. Gülçin and Ö.İ. Küfrevioğlu, 2003. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. LWT Food Sci. Technol., 36: 263-271.
Prakash, D., G. Upadhyay, B.N. Singh and H.B. Singh, 2007. Antioxidant and free radical-scavenging activities of seeds and agri-wastes of some varieties of soybean (Glycine max). Food Chem., 104: 783-790.
Rice-Evans, C.A., N.J. Miller and G. Paganga, 1996. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biol. Med., 20: 933-956.
Roth, K., 2004. [Licorice spirals]. Chemie in unserer Zeit, 38: 202-207, (In German).
Sen, C., L. Packer and O. Hanninen, 2000. Handbook of oxidants and Antioxidants in Exercise. 1st Edn., Elsevier Science B.V., Amsterdam, The Netherlands, ISBN-13: 9780080538297, Pages: 1220.
Siddhuraju, P. and K. Becker, 2003. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J. Agric. Food Chem., 51: 2144-2155.
Stoilova, I., A. Krastanov, A. Stoyanova, P. Denev and S. Gargova, 2007. Antioxidant activity of a ginger extract (Zingiber officinale). Food Chem., 102: 764-770.
Su, M.S., Y.T. Shyu and P.J. Chien, 2008. Antioxidant activities of citrus herbal product extracts. Food Chem., 111: 892-896.
Sultana, B., F. Anwar and R. Przybylski, 2007. Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica and Eugenia jambolana Lam. trees. Food Chem., 104: 1106-1114.
Bukhari, S.B., M.I. Bhanger and S. Memon, 2008. Antioxidative activity of extracts from fenugreek seeds (Trigonella foenum-graecum). Pak. J. Anal. Environ. Chem., 9: 78-83.
Zhishen, J., T. Mengcheng and W. Jianming, 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem., 64: 555-559.
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