New Approach for Lactose Transglycosylation into Galactooligosaccharides

Authors

  • Ivan Fiodorovich Gorlov Volga Region Research Institute of Manufacture and Processing of Meat-and-Milk Production, 400131, Rokossovskogo Street, 6, Volgograd, Russia Volgograd State Technical University, 400005, Lenin Avenue, 28, Volgograd, Russia
  • Andrey Georgievich Khramtsov North Caucasus Federal University, 355029, Kulacova Street, 2, Stavropol, Russia
  • Ivan Alekseevich Evdokimov North Caucasus Federal University, 355029, Kulacova Street, 2, Stavropol, Russia
  • Aleksey Dmitrievich Lodygin North Caucasus Federal University, 355029, Kulacova Street, 2, Stavropol, Russia
  • Anastasia Borisovna Rodnaya North Caucasus Federal University, 355029, Kulacova Street, 2, Stavropol, Russia
  • Marina Ivanovna Slozhenkina Volga Region Research Institute of Manufacture and Processing of Meat-and-Milk Production, 400131, Rokossovskogo Street, 6, Volgograd, Russia Volgograd State Technical University, 400005, Lenin Avenue, 28, Volgograd, Russia
  • Natalia Ivanovna Mosolova Volga Region Research Institute of Manufacture and Processing of Meat-and-Milk Production, 400131, Rokossovskogo Street, 6, Volgograd, Russia
  • Elena Yurievna Zlobina Volga Region Research Institute of Manufacture and Processing of Meat-and-Milk Production, 400131, Rokossovskogo Street, 6, Volgograd, Russia
  • Ekaterina Vladimirovna Karpenko Volga Region Research Institute of Manufacture and Processing of Meat-and-Milk Production, 400131, Rokossovskogo Street, 6, Volgograd, Russia

DOI:

https://doi.org/10.3923/pjn.2015.822.827

Keywords:

Food wastes recycling, galacto-oligosaccharides, lactose-containing dairy raw, transglycosylation, ultrafiltration, whey permeate

Abstract

The purpose of the research was to explore the feasibility of enzymatic catalysis with elements of microbial synthesis of the polymerization of lactose monomers into the galactooligosaccharides (GalOS) nanoclusters. The main objects of the study were the lactose solution (milk raw sugar) and the whey permeates subjected to electroactivation and fermentation by Lactokanestsin G20x and Ha-Lactase. To determine the carbohydrate composition of samples and finished products, the high-pressure liquid chromatography method was used. For the process monitoring, the freezing point depression method and the lactose transformation rate were applied. The directed and controlled types of synthesis of a universal agricultural bioecologic raw material of animal origin were examined in the context of solving a task of transglycosylation of the whey lactose into the GalOS. A biotechnology paradigm of the enzyme-catalyzed GalOS polymerization by [beta]-galactosidase in model solutions of milk sugar and industrial samples of the whey permeates was substantiated. The transglycosylation patterns with optimized parameters of the GalOS synthesis were established. The GalOS synthesis in the whey permeate had an optimum, which was observed at a temperature of (50±2)°C and a pH value of (6.4±0.2). On the basis of the research results, the Innovative Technology Platform (the priority was patented with know-how elements) of the GalOS-concentrates production was generated. The relevance of this work is due not only to the practical applicability of the GalOS-concentrates as prebiotic components and their biological value, but also to the need for the complex processing of lactose containing raw material.

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Published

15.10.2015

Issue

Vol. 14 No. 11 (2015)

Section

Research Article

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Copyright (c) 2015 Asian Network for Scientific Information

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How to Cite

Gorlov, I. F., Khramtsov, A. G., Evdokimov, I. A., Lodygin, A. D., Rodnaya, A. B., Slozhenkina, M. I., Mosolova, N. I., Zlobina, E. Y., & Karpenko, E. V. (2015). New Approach for Lactose Transglycosylation into Galactooligosaccharides. Pakistan Journal of Nutrition, 14(11), 822–827. https://doi.org/10.3923/pjn.2015.822.827