Extraction, Purification, and Inhibitory Effect of Alpha - Amylase Inhibitor from Wheat (Triticum aestivum Var. Zarrin)
DOI:
https://doi.org/10.3923/pjn.2005.101.105Keywords:
Alpha-amylase inhibitor, wheat, ion exchange chromatography, human salivaryAbstract
Plant alpha-amylase inhibitors show great potential as tools to engineer resistance of crop plants against pests. They are also drug-design targets for treatment of diabetes and digestion disorderes. These inhibitors also known as sensitizing agents in human. The numerous form of alpha-amylase inhibitors was reported. In this study alpha-amylase inhibitor was extracted from Iranian wheat cultivar (Triticum aestivum v zarrin), precipitated and purified by anion exchange fast protein liquid chromatography. Electrophoresis of purified protein showed 0.66 relative mobility. Total hydrolytic activity of human salivary and bacillus subtilis alpha-amylase were inhibited 97.07% and 89.97% respectively by collected purified alpha-amylase inhibitor.
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Copyright (c) 2005 Asian Network for Scientific Information
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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
