Research Article Open Access

Evaluation of Mutagenic Profile of Shrimp Paste Extracts by Using Ames Test

Authors

Nur Hafiza Mohammad
Biomedical Science Programme, School of Diagnostic Science and Applied Health, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
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Nurlaily Ezzaty Mohamad Yusoff
Environmental Health and Industrial Safety Programme, School of Diagnostic Science and Applied Health, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
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Siti Shahara Zulfakar
Environmental Health and Industrial Safety Programme, School of Diagnostic Science and Applied Health, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
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CORRESPONDING AUTHOR
Razinah Sharif
Nutrition Science Programme, School of Healthcare Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
Published on: January 15, 2016
DOI 10.3923/pjn.2016.170.175
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Abstract

Shrimp paste (belacan) has been used as a condiment in many Malaysian cooking. This study aimed to determine the mutagenicity of aqueous extract of shrimp paste from three different places in Pulau Pinang, Malaysia (Balik Pulau, Juru and Pulau Aman) by using Ames test. The test was performed using Salmonella typhimurium strain, TA98 and TA100, in presence and absence of S9 metabolic activation system. Five concentrations of the samples tested were 50, 25, 12.5, 6.25 and 3.125 mg/ml. Macronutrients and mineral content of shrimp paste tested were complied with the Malaysian food act 1983 and Recommended Nutrient Intake (RNI) 2005. Ames test result of shrimp paste from all three places showed number of revertant colonies did not exceed the value of double-fold of negative control either in presence and absence of S9. No significant difference (p>0.05) was found for each concentration tested on the number of revertant colonies between TA 98 and TA 100.

Keywords:

Mineral content, mutagenicity, shrimp paste

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Mohammad, N. H., Yusoff, N. E. M., Zulfakar, S. S., & Sharif, R. (2016). Evaluation of Mutagenic Profile of Shrimp Paste Extracts by Using Ames Test. Pakistan Journal of Nutrition, 15(2), 170–175. https://doi.org/10.3923/pjn.2016.170.175
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