Evaluation of Mutagenic Profile of Shrimp Paste Extracts by Using Ames Test
DOI:
https://doi.org/10.3923/pjn.2016.170.175Keywords:
Mineral content, mutagenicity, shrimp pasteAbstract
Shrimp paste (belacan) has been used as a condiment in many Malaysian cooking. This study aimed to determine the mutagenicity of aqueous extract of shrimp paste from three different places in Pulau Pinang, Malaysia (Balik Pulau, Juru and Pulau Aman) by using Ames test. The test was performed using Salmonella typhimurium strain, TA98 and TA100, in presence and absence of S9 metabolic activation system. Five concentrations of the samples tested were 50, 25, 12.5, 6.25 and 3.125 mg/ml. Macronutrients and mineral content of shrimp paste tested were complied with the Malaysian food act 1983 and Recommended Nutrient Intake (RNI) 2005. Ames test result of shrimp paste from all three places showed number of revertant colonies did not exceed the value of double-fold of negative control either in presence and absence of S9. No significant difference (p>0.05) was found for each concentration tested on the number of revertant colonies between TA 98 and TA 100.
References
Abdullah, R., 2012. Assessment of shrimp paste dietary intake and its association with toxicological genetic profile. Master's Thesis, University Kebangsaan Malaysia, Kuala Lumpur.
Ames, B.N., F.D. Lee and W.E. Durston, 1973. An improved bacterial test system for the detection and classification of mutagens and carcinogens. Proc. Natl. Acad. Sci. USA., 70: 782-786.
Armstrong, R.W., M.J. Armstrong, M.C. Yu and B.E. Henderson, 1983. Salted fish and inhalants as risk factors for nasopharyngeal carcinoma in Malaysian Chinese. Cancer Res., 43: 2967-2970.
Armstrong, R.W., P.B. Imrey, M.S. Lye, M.J. Armstrong, M.C. Yu and S. Sani, 1998. Nasopharyngeal carcinoma in Malaysian Chinese: Salted fish and other dietary exposures. Int. J. Cancer, 77: 228-235.
Arshad, F., P.K. Lim, L.H. Yee, S. Nooriah and S. Suzana et al., 2005. A retrospective case-control study: The association between dietary intake and cancer. Department of Nutrition and Dietetics, UKM, Department of Medicine, UM, National Cancer Council, Malaysia.
Cho, M., O.P. Eze and R. Xu, 2013. A brief review of the controversial role of iron in colorectal carcinogenesis. J. Clin. Exp. Pathol., Vol. 3.
Cohen, A.J. and F.J.C. Roe, 1997. Evaluation of the aetiological role of dietary salt exposure in gastric and other cancers in humans. Food Chem. Toxicol., 35: 271-293.
Cunniff, P., 1995. Official Methods of Analysis of Association of Official Analytical Chemists. 16th Edn., AOAC., Arlington, VA., USA.
Dich, J., R. Jarvinen, P. Knekt and P.L. Penttila, 1996. Dietary intakes of nitrate, nitrite and NDMA in the Finnish mobile clinic health examination survey. Food Addit. Contam., 13: 541-552.
Fenech, M., 2003. Nutritional treatment of genome instability: A paradigm shift in disease prevention and in the setting of recommended dietary allowances. Nutr. Res. Rev., 16: 109-122.
Ghazali, A.R., R.M. Halim, N.F. Rajab, N. Ramli, R. Abdullah, F. Kamarulzaman and Z. Harun, 2013. Gene mutation effect of aqueous and methanol extracts of salted fish from pulau pinang, Malaysia towards V79 lung fibroblast cells. Sains Malaysiana, 42: 1599-1603.
Ghazali, A.R., N.F. Rajab, R. Sharif, T.A. Yaakob and F. Arshad, 2005. The genotoxicological evaluation of several local raw foods extracts on chang liver cells by single cell electrophoresis assay. Environ. Mutagen Res., 27: 165-170.
Ghazali, A.R., Y. Sipain, N.F. Rajab, S.E. Ling and N. Ramli et al., 2012. Genotoxic potential of shrimp pastes (Belacan) extracts using Umu test. Food Nutr. Sci., 3: 522-525.
Helen-Ng, L.C., I.A. Razak, W.M.N. Ghani, J. Marhazlinda and A.T. Norain et al., 2012. Dietary pattern and oral cancer risk-a factor analysis study. Commun. Dentistry Oral Epidemiol., 40: 560-566.
Knekt, P., R. Jarvinen, J. Dich and T. Hakulinen, 1999. Risk of colorectal and other gastro-intestinal cancers after exposure to nitrate, nitrite and N-nitroso compounds: A follow-up study. Int. J. Cancer, 80: 852-856.
Kurosawa, M., S. Kikuchi, J. Xu and Y. Inaba, 2006. Highly salted food and mountain herbs elevate the risk for stomach cancer death in a rural area of Japan. J. Gastroenterol. Hepatol., 21: 1681-1686.
Lau, H.Y., C.M. Leung, Y.H. Chan, W.M. Lee and L.W. Kwong et al., 2013. Secular trends of salted fish consumption and nasopharyngeal carcinoma: A multi-jurisdiction ecological study in 8 regions from 3 continents. BMC Cancer, Vol. 13.
Lin, S., X. Wang, C. Huang, X. Liu, J. Zhao, I.T.S. Yu and D.C. Christiani, 2015. Consumption of salted meat and its interactions with alcohol drinking and tobacco smoking on esophageal squamous-cell carcinoma. Int. J. Cancer, 137: 582-589.
Lin, S.H., Y.H. Li, K. Leung, C.Y. Huang and X.R. Wang, 2014. Salt processed food and gastric cancer in a Chinese population. Asian Pac. J. Cancer Prev., 15: 5293-5298.
MKM., 2008. Evaluation of biochemical profile of several local raw food extracts. Majlis Kanser Malaysi (MKM), Malaysia.
Malaysian Food Act, 2004. Food Act 1983 (Act 281) and Regulations. International Law Book Services, Kuala Lumpur, Malaysia.
Manson, M.M. and D.J. Benford, 1999. Factors influencing the carcinogenicity of food chemicals. Toxicology, 134: 93-108.
Maron, D.M. and B.N. Ames, 1983. Revised methods for the Salmonella mutagenicity test. Mutat. Res./Environ. Mutagen. Relat. Subj., 113: 173-215.
McNaught, A.D. and A. Wilkinson, 1997. IUPAC Gold Book: Compendium of Chemical Terminology. 2nd Edn., Blackwell Scientific Publications, London, UK.
Mortelmans, K. and E. Zeiger, 2000. The Ames Salmonella/microsome mutagenicity assay. Mutat. Res./Fundam. Mol. Mech. Mutagen., 455: 29-60.
Murata, A., Y. Fujino, T.M. Pham, T. Kubo and T. Mizoue et al., 2010. Prospective cohort study evaluating the relationship between salted food intake and gastrointestinal tract cancer mortality in Japan. Asia Pac. J. Clin. Nutr., 19: 564-571.
Peleteiro, B., C. Lopes, C. Figueiredo and N. Lunet, 2011. Salt intake and gastric cancer risk according to Helicobacter pylori infection, smoking, tumour site and histological type. Br. J. Cancer, 104: 198-207.
Phukan, R.K., K. Narain, E. Zomawia, N.C. Hazarika and J. Mahanta, 2006. Dietary habits and stomach cancer in Mizoram, India. J. Gastroenterol., 41: 418-424.
Sharif, R., A.R. Ghazali, N.F. Rajab, H. Haron and F. Osman, 2008. Toxicological evaluation of some Malaysian locally processed raw food products. Food Chem. Toxicol., 46: 368-374.
Skog, K.I., M.A.E. Johansson and M.I. Jagerstad, 1998. Carcinogenic heterocyclic amines in model systems and cooked foods: A review on formation, occurrence and intake. Food Chem. Toxicol., 36: 879-896.
Sumino, T., S. Kageyama, E. Endo, R. Chihara and K. Yamada, 2003. Components and bacterial content of belacan produced in Malaysia. J. Cookery Sci. Jpn., 36: 164-169.
Takachi, R., M. Inoue, T. Shimazu, S. Sasazuki and J. Ishihara et al., 2010. Consumption of sodium and salted foods in relation to cancer and cardiovascular disease: The Japan public health center-based prospective study. Am. J. Clin. Nutr., 91: 456-464.
Tatematsu, M., M. Takahashi, S. Fukushima, M. Hananouchi and T. Shirai, 1975. Effects in rats of sodium chloride on experimental gastric cancers induced by N-methyl-N-nitro-N-nitrosoguanidine or 4-nitroquinoline-1-oxide. J. Natl. Cancer Inst., 55: 101-106.
Tsugane, S., S. Sasazuki, M. Kobayashi and S. Sasaki, 2004. Salt and salted food intake and subsequent risk of gastric cancer among middle-aged Japanese men and women. Br. J. Cancer, 90: 128-134.
Tsugane, S., 2005. Salt, salted food intake and risk of gastric cancer: Epidemiologic evidence. Cancer Sci., 96: 1-6.
Vanita, P., V. Subrahmanyam and K. Jhansi, 2011. A short of cancer. J. Carcinog. Mutagen., 2: 128-128.
Vargas, A.J. and P.A. Thompson, 2012. Diet and nutrient factors in colorectal cancer risk. Nutr. Clin. Pract., 27: 613-623.
Pongsetkul, J., S. Benjakul, P. Sampavapol, K. Osako and N. Faithong, 2014. Chemical composition and physical properties of salted shrimp paste (Kapi) produced in Thailand. Int. Aquat. Res., 6: 155-166.
Rohi, A.G., R.N. Fadilah, S. Razinah, H. Hasnah and O. Fadzil, 2005. Levels of contaminants in several local raw food products. J. Environ. Health, 2: 18-21.
Ruddle, K. and N. Ishige, 2009. On the Origins, Diffusion and Cultural Context of Fermented Fish Products in Southeast Asia. In: Globalization, Food and Social Identities in the Asia Pacific Region, Farrer, J. (Ed.). Sophia University Institute of Comparative Culture, Tokyo.
Sakanaka, S., Y. Tachibana and Y. Okada, 2005. Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha). Food Chem., 89: 569-575.
Wang, X.Q., H. Yan and P.D. Terry, 2009. Review of salt consumption and stomach cancer risk: Epidemiological and biological evidence. World J. Gastroenterol., 15: 2204-2213.
WCRF. and AICR., 2007. Food, Nutrition, Physical Activity and the Prevention of Cancer: A Global Perspective. World Cancer Research Fund/American Institute of Cancer Research, Washington, DC., USA., ISBN-13: 9780972252225, Pages: 517.
WHO. and FAO., 2003. Diet, nutrition and the prevention of chronic diseases: Report of a joint WHO/FAO expert consultation, Geneva, January 28-February 1, 2002. WHO Technical Report Series 916, FAO/WHO, Geneva, Switzerland, pp: 1-149.
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