Tomato Extract as an Immunomodulator in Mice (Mus musculus) Infected with Plasmodium berghei
DOI:
https://doi.org/10.3923/pjn.2016.515.518Keywords:
Interleukin-12, lycopene, malaria, Plasmodium berghei, tomato extractAbstract
To prevent malaria, it is necessary to increase the immune response in the body and reduce disease severity by eradicating the parasites within the body. Tomato (Lycopersicum esculentum Mill) is believed to be able to increase the immune response to combat infection. The current study examined 5 different treatment conditions and each treatment was repeated 6 times. The negative control group (K-) was given standard nourishment, while the positive control group (K+) received both standard nourishment and chloroquine treatment. Groups P1, P2 and P3 were given standard nourishment, as well as a dose of 0.1, 1 and 10 mg/kg BW (body weight)/day tomato extract, respectively. Each group of mice was intraperitoneally infected with 107 cells/mL Plasmodium berghei. On the 8th day post infection, the mice were sacrificed and lymphocytes and macrophages were isolated and subsequently cultured. The expression levels of IL-12 and IFN-γ, as well as the macrophage phagocytosis index of the cells, were then determined. IL-12 levels were significantly different (p<0.05) between the groups that were given tomato extract (P2 and P3) and the groups that did not receive tomato extract (K-and K+). However, the mice that received 0.1 mg/kg BW tomato extract were not significantly different from those of the K- and K+ groups. The results of this study suggest that the most effective dose of tomato extract is 10 mg/kg BW.
References
Rao, A.V. and S. Agarwal, 2000. Role of antioxidant lycopene in cancer and heart disease. J. Am. Coll. Nutr., 19: 563-569.
Belnoue, E., F.T.M. Costa, T. Frankenberg, A.M. Vigario and T. Voza et al., 2004. Protective T cell immunity against malaria liver stage after vaccination with live sporozoites under chloroquine treatment. J. Immunol., 172: 2487-2495.
Burri, B.J. and A.J. Clifford, 2004. Carotenoid and retinoid metabolism: Insights from isotope studies. Arch. Biochem. Biophys., 430: 110-119.
Duriancik, D.M., D.E. Lackey and K.A. Hoag, 2010. Vitamin A as a regulator of antigen presenting cells. J. Nutr., 140: 1395-1399.
Frevert, U. and E. Nardin, 2008. Cellular effector mechanisms against Plasmodium liver stages. Cell. Microbiol., 10: 1956-1967.
Zapata-Gonzalez, F., F. Rueda, J. Petriz, P. Domingo, F. Villarroya, A. de Madariaga and J.C. Domingo, 2007. 9-cis-Retinoic acid (9cRA), a retinoid X receptor (RXR) ligand, exerts immunosuppressive effects on dendritic cells by RXR-dependent activation: Inhibition of peroxisome proliferator-activated receptor γ blocks some of the 9cRA activities and precludes them to mature phenotype development. J. Immunol., 178: 6130-6139.
Hall, J.A., J.R. Grainger, S.P. Spencer and Y. Belkaid, 2011. The role of retinoic acid in tolerance and immunity. Immunity, 35: 13-22.
Iribhogbe, O.I., E.O. Agbaje, I.A. Oreagba, O.O. Aina and A.D. Ota, 2012. Oxidant versus antioxidant activity in malaria: Role of nutritional therapy. J. Med. Sci., 12: 229-233.
Lobo, G.P., S. Hessel, A. Eichinger, N. Noy and A.R. Moise et al., 2010. ISX is a retinoic acid-sensitive gatekeeper that controls intestinal β, β-carotene absorption and vitamin A production. FASEB J., 24: 1656-1666.
Metzger, A., G. Mukasa, A.H. Shankar, G. Ndeezi, G. Melikian and R.D. Semba, 2001. Antioxidant status and acute malaria in children in Kampala, Uganda. Am. J. Trop. Med. Hyg., 65: 115-119.
MoH., 2014. Annual report of Indonesian health profile. Ministry of Health (MoH), Kemenkes, Jakarta.
Nugroho, A., P.N. Harijanto and E.A. Datau, 2000. Immunology of Malaria. In: Malaria: Epidemiology, Pathogenicity, Clinical Manifestation and Cases Handling, Harijanto, P.N. (Ed.). EGC, Jakarta.
Raverdeau, M. and K.H.G. Mills, 2014. Modulation of T cell and innate immune responses by retinoic acid. J. Immunol., 192: 2953-2958.
Rosales, F.J., J.D. Topping, J.E. Smith, A.H. Shankar and A.C. Ross, 2000. Relation of serum retinol to acute phase proteins and malarial morbidity in Papua New Guinea children. Am. J. Clin. Nutr., 71: 1582-1588.
Ross, A.C., 2012. Vitamin A and retinoic acid in T cell-related immunity. Am. J. Clin. Nutr., 96: 1166S-1172S.
Paiva, S.A. and R.M. Russell, 1999. β-carotene and other carotenoids as antioxidants. J. Am. Coll. Nutr., 18: 426-433.
Takeuchi, H., A. Yokota, Y. Ohoka, H. Kagechika, C. Kato, S.Y. Song and M. Iwata, 2010. Efficient induction of CCR9 on T cells requires coactivation of retinoic acid receptors and retinoid X receptors (RXRs): Exaggerated T Cell homing to the intestine by RXR activation with organotins. J. Immunol., 185: 5289-5299.
Artavanis-Tsakonas, K. and E.M. Riley, 2002. Innate immune response to malaria: Rapid induction of IFN-γ from human NK cells by live Plasmodium falciparum-infected erythrocytes. J. Immunol., 169: 2956-2963.
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