Chemical Composition, Flavonoids and β-sitosterol Contents of Pulp and Rind of Watermelon (Citrullus lanatus) Fruit
DOI:
https://doi.org/10.3923/pjn.2017.502.507Keywords:
Anti-oxidants, β-carotene, chemical composition, citrullus lanatus, flavonoids, phytosterolsAbstract
Background: Recently, there is increasing demand for consumption of natural and healthy foods. Fruits (and watermelon among them) are believed to contain variety of dietary phytochemicals that may have beneficial effect on human health. Objective: The aim of this study was to prepare three samples from watermelon fruit (of Saudi origin ) and to determine proximate composition, mineral, vitamin, flavonoids and beta sitosterol contents of the three samples. Methodology: Three samples were prepared from interior pulp region, exterior pulp region and from the rind of watermelon fruit. Proximate composition was determined by conventional official methods, analytical determination of minerals was performed by ICP-MS (inductively coupled plasma-mass spectrophotometer), vitamins and flavonoids were determined by High Performance Liquid Chromatography (HPLC) and β-sitosterol by Gas Chromatography (GC) techniques. Statistical analysis was performed by using SPSS software. Results: Moisture (ranged from 85-95%) was the predominant component in the samples. Carbohydrates ranged from 62.00-87.14% in dry weight basis, while fat content was low in the three watermelon samples. Potassium was the predominant mineral and ranged from 100.50-489.24 mg/100 g, vitamin C ranged between 2.50-8.30 mg/100 g, vitamin B6 0.060-0.150 mg/100 g and vitamin E 0.01-0.04 mg/100 g in the three watermelon samples. β-carotene concentration was high in the interior pulp (0.610 mg/100 g) compared to that in the rind (0.120 mg/100 g). Rutin was the only flavonoid detected in watermelon fruit, rutin concentration in the interior pulp sample was 1.66 mg/100 g. β-sitosterol is a phytosterol found in all parts of watermelon fruit, ranging from 0.140-0.627 mg/100 g. Conclusion: The results of this study may highlight the potential of watermelon fruit as a source of nutrients and anti-oxidants that may be considered for human nutrition and health.
References
Wani, A.A., D.S. Sogi, P. Singh, I.A. Wani and U.S. Shivhare, 2011. Characterisation and functional properties of watermelon (Citrullus lanatus) seed proteins. J. Sci. Food Agric., 91: 113-121.
FAOSTAT., 2016. Statistics. Food and Agriculture Organization of United Nations, Rome, Italy.
Romdhane, M.B., A. Haddar, I. Ghazala, K.B. Jeddou, C.B. Helbert and S. Ellouz-Chaabouni, 2017. Optimization of polysaccharides extraction from watermelon rinds: Structure, functional and biological activities. Food Chem., 216: 355-364.
Arshiya, S., 2013. The antioxidant effect of certain fruits: A review. J. Pharm. Sci. Res., 5: 265-268.
ESHA., 2017. The food processor, version 10.12.0. Watermelon Nutritional Profile, ESHA Research, Salem, Oregan, USA.
Perkins-Veazie, P., J.K. Collins and B. Chevidence, 2007. Watermelons and health. Acta Hortic., 731: 121-128.
Tlili, I., C. Hdider, M.S. Lenucci, I. Ridah, H. Jebari and G. Dalessandro, 2011. Bioactive compounds and antioxidant activities of different watermelon (Citrullus lanatus (Thunb.) Mansfeld) cultivars as affected by fruit sampling area. J. Food Compos. Anal., 24: 307-314.
Miean, K.H. and S. Mohamed, 2001. Flavonoid (myricetin, quercetin, kaempferol, luteolin and apigenin) content of edible tropical plants. J. Agric. Food Chem., 49: 3106-3112.
Yoo, K.S., E.J. Lee and B.S. Patil, 2010. Quantification of quercetin glycosides in 6 onion cultivars and comparisons of hydrolysisâ€HPLC and spectrophotometric methods in measuring total quercetin concentrations. J. Food Sci., 75: C160-C165.
Al-Dhabi, N.A., M.V. Arasu, C.H. Park and S.V. Park, 2015. An up-to-date review of rutin and its biological and pharmacological activities. EXCLI J., 14: 59-63.
Saeidnia, S., A. Manayi, A.R. Gohari and M. Abdollahi, 2014. The story of β-sitosterol-A review. Eur. J. Med. Plants, 4: 590-609.
Figueroa, A., M.A. Sanchez-Gonzalez, P.M. Perkins-Veazie and B.H. Arjmandi, 2011. Effects of watermelon supplementation on aortic blood pressure and wave reflection in individuals with prehypertension: A pilot study. Am. J. Hypertens., 24: 40-44.
Poduri, A., D.L. Rateri, S.K. Saha, S. Saha and A. Daugherty, 2013. Citrullus lanatus sentinel (watermelon) extract reduces atherosclerosis in LDL receptor-deficient mice. J. Nutr. Biochem., 24: 882-886.
AOAC., 2005. Official Methods of Analysis. 18th Edn., Association of Official Analytical Chemists, Washington, DC., USA.
Sami, R., Y. Li, B. Qi, S. Wang and Q. Zhang et al., 2014. HPLC analysis of water-soluble vitamins (B2, B3, B6, B12 and C) and fat-soluble vitamins (E, K, D, A and β-carotene) of Okra (Abelmoschus esculentus). J. Chem.
Olszewska, M., 2007. Quantitative HPLC analysis of flavonoids and chlorogenic acid in the leaves and inflorescences of Prunus serotina Ehrh. Acta Chromatographica, 19: 253-269.
USDA., 2003. Chemical composition of watermelon. USDA Food Composition Databases, Agricultural Research Service, United States Department of Agriculture, USA.
Al-Sayed, H.M.A. and A.R. Ahmed, 2013. Utilization of watermelon rinds and sharlyn melon peels as a natural source of dietary fiber and antioxidants in cake. Ann. Agric. Sci., 58: 83-95.
Malkki, Y., 2001. Physical properties of dietary fiber as keys to physiological functions. Cereal Foods World, 46: 196-199.
Leterme, P., A. Buldgen, F. Estrada and A.M. Londono, 2006. Mineral content of tropical fruits and unconventional foods of the Andes and the rain forest of Colombia. Food Chem., 95: 644-652.
Huang, Y., L. Zhao, Q. Kong, F. Cheng and M. Niu et al., 2016. Comprehensive mineral nutrition analysis of watermelon grafted onto two different rootstocks. Hortic. Plant J., 2: 105-113.
Johnson, J.T., J.A. Lennox, V.P. Ujong, M.O. Odey, W.O. Fila, P.N. Edim and K. Dasofunjo, 2013. Comparative vitamins content of pulp, seed and rind of fresh and dried watermelon (Citrullus lanatus). Int. J. Sci. Technol., 2: 99-103.
Chun, J., J. Lee, L. Ye, J. Exler and R.R. Eitenmiller, 2006. Tocopherol and tocotrienol contents of raw and processed fruits and vegetables in the United States diet. J. Food Comp. Anal., 19: 196-204.
Atanassova, M. and V. Bagdassarian, 2009. Rutin content in plant products. J. Univ. Chem. Technol. Metallurgy, 44: 201-203.
Seo, S., M.S. Lee, E. Chang, Y. Shin, S. Oh, I.H. Kim and Y. Kim, 2015. Rutin increases muscle mitochondrial biogenesis with AMPK activation in high-fat diet-induced obese rats. Nutrients, 7: 8152-8169.
Duester, K.C., 2001. Avocado fruit is a rich source of β-sitosterol. J. Am. Dietetic Assoc., 101: 404-405.
Moghadasian, M.H. and J.J. Frohlich, 1999. Effects of dietary phytosterols on cholesterol metabolism and atherosclerosis: Clinical and experimental evidence. Am. J. Med., 107: 588-594.
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