Effect of Sukun Leaf Extract [Artocarpus altilis (Park.) Fosberg] on Insulin Resistance in Obese Rats (Rattus norvegicus): A Study of Free Fatty Acid (FFA) Levels
DOI:
https://doi.org/10.3923/pjn.2017.521.524Keywords:
A. altilis, FFA, insulin resistance, obesityAbstract
Background and Objective: The state of obesity is closely related to insulin resistance, which leads to type 2 diabetes. Free Fatty Acids (FFAs) play a role in the development of insulin resistance. Artocarpus altilis plants have traditionally been used by the people of Indonesia for the treatment of diabetes mellitus. This study aimed to determine the concentrations of A. altilis leaf extract that can improve insulin resistance in obese rats (Rattus norvegicus) through identification of the concentrations that can decrease the levels of FFA. Methodology: This study is a Randomized Controlled Trial (RCT). The rats were divided into five groups, each consisting of five rats. The rats were administered a high-fat diet (45%) (open source) to induce fattening and their weights were measured for assessing obesity. The fasting blood sugar (GDP) was monitored through gluco DR tests to ensure that the mice exhibited an increased glucose concentration. Group I was designated the negative control group, the rats in group II were administered metformin HCl and formed the positive control group and groups III, IV and V were denoted the 5, 10 and 15% A. altilis test groups. The groups underwent treatment for 14 days and ELISA was performed for the assessment of the FFA levels in each group after testing. A data analysis using nonparametric tests was performed to assess the significance of the differences among the groups at a 95% confidence level. All of the data were analyzed with SPSS version 21.0 (SPSS, Inc., Chicago, IL, USA). Results: The FFA levels in the negative control and the10% A. altilis (p = 0.000) and 15% A. altilis (p = 0.016) extracts did not show any significant differences, differences in the FFA content were found between the positive control and the 5% A. altilis (0.034) and 10% A. altilis (p = 0.020) test groups. Conclusion: The 10% A. altilis extract can lower the FFA levels in obese mice. Therefore, A. altilis should be considered for use in the prevention of insulin resistance.
References
Savage, D.B., K.F. Petersen and G.I. Shulman, 2005. Mechanisms of insulin resistance in humans and possible links with inflammation. Hypertension, 45: 828-833.
Kahn, B.B. and J.S. Flier, 2000. Obesity and insulin resistance. J. Clin. Invest., 106: 473-481.
Gallagher, E.J., D. Leroith and E. Karnieli, 2010. Insulin resistance in obesity as the underlying cause for the metabolic syndrome. Mt. Sinai J. Med., 77: 511-523.
Boden, G., 2008. Obesity and free fatty acids. Endocrinol. Metab. Clin. North Am., 37: 635-646.
Ceriello, A. and E. Motz, 2004. Is oxidative stress the pathogenic mechanism underlying insulin resistance, diabetes and cardiovascular disease? The common soil hypothesis revisited. Arteriosclerosis Thrombosis Vasc. Biol., 24: 816-823.
Henry, R.R. and S. Mudaliar, 2003. Obesity and Type II Diabetes Mellitus. In: Obesity: Mechanisms and Clinical Management, Eckel, R.H. (Ed.). Lippincott Williams and Wilkins, Philadelphia, pp: 229-272.
Boden, G., 2001. Pathogenesis of type 2 diabetes: Insulin resistance. Endocrinol. Metabol. Clin. North Am., 30: 801-815.
Boden, G., 2011. Obesity, insulin resistance and free fatty acids. Curr. Opin. Endocrinol. Diabetes Obes., 18: 139-143.
Farabi, M.Z., 2013. Qualitative analysis of active substance content on breadfruit leaf extract (Artocarpus altilis). University of Muhammadiyah, Semarang.
Maharani, T.W., 2011. Phytochemical test of leaf bread leaf extract (Artocarpus altilis). University of Muhammadiyah, Semarang.
Kaneto, H., Y. Kajimoto, J. Miyagawa, T. Matsuoka and Y. Fujitani et al., 1999. Beneficial effects of antioxidants in diabetes: Possible protection of pancreatic beta-cells against glucose toxicity. Diabetes, 48: 2398-2406.
Ferrannini, E. and A. Mari, 2004. Beta cell function and its relation to insulin action in humans: A critical appraisal. Diabetologia, 47: 943-956.
Corwin, E.J., 2006. Handbook of Pathophysiology. Lippincott Williams and Wilkins, USA.
Kumar, V., A.K. Abbas, N. Fausto, S.L. Robbins and R.S. Cotran, 2005. Robbins and Cotran Pathologic Basis of Disease. 7th Edn., Elsevier Saunders, Philadelphia, ISBN-10: 0721601871, Pages: 1525.
Chattopadhyay, R.R. and M. Bandyopadhyay, 2005. Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract against paracetamol-induced hepatic damage in rats: Part III. Indian J. Pharmacol., 37: 184-185.
Sherwood, L., 2004. Human Physiology: From Cells to System. 5th Edn., Thomson Learning, Belmont, CA.
Brahmachari, G., 2011. Bio-Flavonoids with Promising Anti-Diabetic Potentials: A Critical Survey. In: Opportunity, Challenge and Scope of Natural Products Medicine Chemistry, Tiwari, V.K. and B.B. Mishra (Eds.). Research Signpost, Kerala, India, pp: 187-212.
Martinez, S.C., C. Cras-Meneur, E. Bernal-Mizrachi and M.A. Permutt, 2006. Glucose regulates Foxo1 through insulin receptor signaling in the pancreatic islet β-cell. Diabetes, 55: 1581-1591.
Gerritsen, M.E., W.W. Carley, G.E. Ranges, C.P. Shen, S.A. Phan, G.F. Ligon and C.A. Perry, 1995. Flavonoids inhibit cytokine-induced endothelial cell adhesion protein gene expression. Am. J. Pathol., 147: 278-292.
Yani, R.W.E., A. Mallongi, S. Andarini, D. Prijatmoko and I.R. Dewanti, 2016. The effect of zinc saliva on the toddlers nutritional status. J. Int. Dental Med. Res., 9: 29-32.
Manjeet, K.R. and B. Ghosh, 1999. Quercetin inhibits LPS-induced nitric oxide and tumor necrosis factor-alpha production in murine macrophages. Int. J. Immunopharmacol., 21: 435-443.
Syam, A., M. Syafar, R. Amiruddin, Muzakkir and Darwis et al., 2017. Early breastfeeding initiation: Impact of socio-demographic, knowledge and social support factors. Pak. J. Nutr., 16: 207-215.
Cartailler, J.P., 2004. Insulin: From secretion to action. The Beta Cell Consortium. http://www.betacell.org/content/articleview/article_id/1/pa ge/1/glossary/1/.
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