Production and Quality Evaluation of Probiotic Beef Sausage
DOI:
https://doi.org/10.3923/pjn.2019.686.691Keywords:
Beef sausage, fungi, lactic acid bacteria, meat industry, probiotic microbesAbstract
Background and objectives: The growing concern of consumers regarding food health and safety issues has led to the development of products that promote health and well-being beyond its nutritional effect. The objective of this study was to develop fermented sausages, incorporating probiotic bacteria as well as determine the viability, bio-chemical qualities and acceptability of the sausage. Materials and Methods: Fermented Sausages were produced from beef with the addition of Lactobacillus acidophilus (L. acidophilus) and Streptococcus thermophilus (S. thermophilus) as probiotics. The quality was compared with control fermented beef sausage, without the addition of probiotics. Both sausage samples were stored at 10-15°C for 10 weeks. The chemical properties, microbial quality, probiotic viability and sensory acceptance of the sausages were evaluated. Results: The results of the chemical composition showed that the probiotic sausages had higher protein (30.28%), ash (11.07%) and carbohydrate (19.02%) contents and lower moisture (31.80%) and fat (7.90%) values than the non probiotic sausage (CS). The pH ranges of the probiotic sausage (5.34-4.11) and control sausage (5.88-5.70) decreased during storage. The percentage lactic acid content of the probiotic sausage was higher (1.35%) than that of the control sausage (0.761%) after storage. The total bacterial counts increased for both probiotic sausage (5.40-7.80 log CFU g–1) and control sausage (8.11-9.10 log CFU g–1) during storage. The probiotics were viable in the fermented sausage during storage with higher population in probiotic sausage (5.98-9.32 log CFU g–1) than that of the control sausage (2.48-2.65 log CFU g–1). The sensory preference evaluation revealed that probiotic beef sausage was more preferred in terms of texture, colour, taste and overall acceptability. Conclusion: The use of probiotic bacteria (L. acidophilus and S. thermophilus) in the production of dry fermented sausage would be beneficial in the meat industry as probiotics delivery vehicles.
References
Onwuka, G.I., 2014. Food Science and Technology. Naphtali Prints, Somolu Lagos.
Granato, D., G.F. Branco, F. Nazzaro, A.G. Cruz and J.A. Faria, 2010. Functional foods and nondairy probiotic food development: Trends, concepts and products. Comprehen. Rev. Food Sci. Food Safety, 9: 292-302.
Ndife, J., 2016. Functional Foods: Basics, Ingredients and Application. Amotees Link Services and Publishers, Kaduna, Nigeria.
Alshmlan, N., 2017. Knowledge, experience and use of probiotics among the universities and (Paaet) students of Kuwait. Eur. J. Food Sci. Technol., 5: 9-34.
Wildman, R.E.C., 2006. Handbook of Nutraceuticals and Functional Foods. CRC Press, Boca Raton FL.
Onuoha, C. and J. Ndife, 2015. Food Processing Additives. Amotes Link Services and Publishers, Kaduna, Nigeria.
Ogunremi, O.R., R. Agrawal and A.I. Sanni, 2015. Development of cereal-based functional food using cereal-mix substrate fermented with probiotic strain-Pichia kudriavzevii OG 32. Food Sci. Nutr., 3: 486-494.
Allen, S.J., E.G. Martinez, G.V. Gregorio and L.F. Dans, 2010. Probiotics for treating acute infectious diarrhoea. Cochrane Database Syst. Rev.
Farnworth, E.R., 2008. Handbook of Fermented Functional Foods. CRC Press, USA., ISBN: 9781420053265, Pages: 600.
Visakh, P.M., L. Iturriaga and P. Ribotta, 2014. Advances in Food Science and Nutrition. Scrivener Publishing, Beverly MA., USA.
Onwuka, G.L., 2005. Food Analysis and Instrumentation: Theory and Practice. 2nd Edn., Naphthali Prints, Lagos, Nigeria.
AOAC., 2005. Official Method of Analysis. 18th Edn., Association Official Analitycal Chemistry, Maryland, USA.
APHA., 1992. Compendium of Methods for the Microbiological Examination of Foods. 3rd Edn., American Public Health Association, Washington, DC., USA.
Iwe, M.O., 2010. Handbook of Sensory Methods and Analysis. 2nd Edn., Rojoint Communication Services Ltd., Enugu, Nigeria.
Abdolghafour, B. and A. Saghir, 2014. Development in sausage production and practices-A review. Meat Sci. Technol., 2: 40-50.
Ezeama, C.F., 2007. Food Microbiology: Fundamentals and Applications. Natural Prints Ltd., Lagos, Nigeria.
Collins, J.K., G. Thornton and G.O. Sullivan, 1998. Selection of probiotic strains for human applications. Int. Dairy J., 8: 487-490.
Chow, J., 2002. Probiotics and prebiotics: A brief overview. J. Renal Nutr., 12: 76-86.
Lu, Y., O.A. Young and J.D. Brooks, 2014. Physicochemical and sensory characteristics of fermented sheepmeat sausage. Food Sci. Nutr., 2: 669-675.
Blagoeva, G., M. Milev, S. Minkova, V. Gotcheva and A. Angelov, 2014. Assessment of lactic acid bacteria and Enterobacteriaceae counts in Bulgarian probiotic products by TEMPO system and ISO methods. J. Nutr. Health Food Eng., Vol. 1, No. 5.
Ahmad, S. and Q. Nawab, 2014. Quality evaluation of fermented sausages as influenced by different fat levels and temperature of fermentation. J. Meat Sci. Technol., 2: 51-62.
Aramide, A.A., S.H. Abiose and A.H. Adeniran, 2009. Microbial evaluation of probiotic beverage from roselle extract. Afr. J. Food Sci., 3: 385-392.
Perez-Chabela, M.D.L., A. Totosaus and I. Guerrero, 2008. Evaluation of thermotolerant capacity of lactic acid bacteria isolated from commercial sausages and the effects of their addition on the quality of cooked sausages. Food Sci. Technol., 28: 132-138.
Bhadoria, P.B.S. and S.C. Mahapatra, 2011. Prospects, technological aspects and limitations of probiotics-A worldwide review. Eur. J. Food Res. Rev., 1: 23-42.
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