Phytochemical Screening and Pharmacological Activities of the Ethanolic Stem Extract of Cleome gynandra
DOI:
https://doi.org/10.3923/pjn.2020.153.159Keywords:
antioxidants, Cleome gynandra, membrane stabilizing, oxidative stress, stem extractAbstract
Objective: This study aimed to identify the phytochemical compounds of the ethanolic stem extract of Cleome gynandra (CGEE) and to determine the antioxidant, membrane stability and analgesic activities of CGEE. Materials and Methods: Phytochemical analysis was conducted qualitatively. Content of total phenolic compounds and tannins was determined using Folin Ciocalteau reagent. Antioxidant activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl assay, membrane stabilizing activity was tested using hypotonic solution and heat-induced hemolysis and analgesic activities were evaluated using the acetic acid-induced writhing test in mice. Results: CGEE is rich in tannins, flavonoids, steroids, glycosides, acidic compounds and proteins. The total phenolic and tannin contents were 237.92 mg gallic acid equivalents (GAE)/100 g and 21.07 mg GAE/100 g of dried plant extract, respectively. CGEE also displayed promising DPPH free radical scavenging activity, with an IC50 value of 9.62 μg mL–1. In the hypotonic solution-induced hemolysis test, the extract showed 43.67, 42.85 and 38.66% inhibition at 0.5, 1.0 and 2.0 mg mL–1, respectively, whereas the standard resulted in 30.57% inhibition. In the heat-induced hemolysis test, 1 mg mL–1 of extract resulted in 84.43% inhibition of hemolysis. Furthermore, in the analgesic activity test, CGEE doses of 250 and 500 mg kg–1 body weight resulted in good inhibition of the writhing reflex (40.87 and 53.92%, respectively), while the standard drug (diclofenac sodium) at 25 mg kg–1 body weight resulted in inhibition of 64.35%. Conclusion: Results of this study suggest that the stem extract of Cleome gynandra possesses antioxidant, membrane stabilizing and analgesic activities.
References
Fang, Y.Z., S. Yang and G. Wu, 2002. Free radicals, antioxidants, and nutrition. Nutrition, 18: 872-879.
Uttara, B., A.V. Singh, P. Zamboni and R.T. Mahajan, 2009. Oxidative stress and neurodegenerative diseases: A review of upstream and downstream antioxidant therapeutic options. Curr. Neuropharmacol., 7: 65-74.
Shyur, L.F., J.H. Tsung, J.H. Chen, C.Y. Chiu and C.P. Lo, 2005. Antioxidant properties of extracts from medicinal plants popularly used in Taiwan. Int. J. Applied Sci. Eng., 3: 195-202.
Chatterjee, S., 2016. Oxidative Stress, Inflammation and Disease. In: Oxidative Stress and Biomaterials. Dziubla, T. and D.A. Butterfield, Academic Press, United States, pp: 35-58.
Gillham, B., D. Papachristodoulou and J. Thomas, 1997. Free Radicals in Health and Disease. 7th Edn., Butterworth Heinemann, Oxford.
Chakraborty, P., S. Kumar, D. Dutta and V. Gupta, 2009. Role of antioxidants in common health diseases. Res. J. Pharm. Technol., 2: 238-244.
Oyedapo, O.O., B.A. Akinpelu, K.F. Akinwunmi, M.O. Adeyinka and F.O. Sipeolu, 2010. Red blood cell membrane stabilizing potentials of extracts of Lantana camara and its fractions. Int. J. Plant Physiol. Biochem., 2: 46-51.
Vadivu, R. and K.S. Lakshmi, 2008. In vitro and in vivo anti-inflammatory activity of leaves of Symplocos cochinchnensis (Lour) Moore ssp. Laurina. Bangladesh J. Pharmacol., 3: 121-124.
Abd El-Kawy, M.A., S. El-Deib, Z. El-Khyat and Y.A. Mikhail, 2000. Chemical and biological studies of cleome droserifolia (Forssk.) Del. part I. Egypt. J. Biomed. Sci., 6: 204-215.
Government of India, Ministry of Ayush, 2016. The Ayurvedic Pharmacopoeia of India Part 1. Vol. 9, 1st Edn., Pharmacopoeia Commission for Indian Medicine and Homeopathy, New Delhi, India.
Anbazhagi, T., K. Kadavul, G. Suguna and A.J.A. Petrus, 2009. Studies on the pharmacognostical and in vitro antioxidant potential of Cleome gynandra Linn. leaves. Nat. Prod. Rad., 8: 151-157.
Moyo, M., S.O. Amoo, B. Ncube, A.R. Ndhlala, J.F. Finnie and J. van Staden, 2013. Phytochemical and antioxidant properties of unconventional leafy vegetables consumed in southern Africa. S. Afr. J. Bot., 84: 65-71.
Nicola, W.G., K.M. Ibrahim, T.H. Mikhail, R.B. Girgis and M.E. Khadr, 1996. Role of the hypoglycemic plant extract cleome droserifolia in improving glucose and lipid metabolism and its relation to insulin resistance in fatty liver. Boll. Chim. Farm., 135: 507-517.
Bala, A., P. Chetia, N. Dolai, B. Khandelwal and P.K. Haldar, 2014. Cat's whiskers flavonoid attenuated oxidative DNA damage and acute inflammation: Its importance in lymphocytes of patients with rheumatoid arthritis. Inflammopharmacology, 22: 55-61.
Yang, R.Y., S. Lin and G. Kuo, 2008. Content and distribution of flavonoids among 91 edible plant species. Asia Pac. J. Clin. Nutr., 17: 275-279.
Ghani, A., 2003. Medicinal plants of Bangladesh: Chemical Constituents and Uses, 2nd Edn., Asiatic Society of Bangladesh, Dhaka, Bangladesh, Pages: 460.
Hossain, A., F. Islam, M. Saifuzzaman, M.A.S. Saeed, M.K. Islam, G.M.M. Murshid and M.M. Rahman, 2016. Bioactivity of Boehmeria macrophylla (Urticaceae) leaf extract. Orien. Pharm. Exp. Med., 16: 233-241.
Islam, T., A. Das, K.B. Shill, P. Karmakar, S. Islam and M.M. Sattar, 2015. Evaluation of membrane stabilizing, anthelmintic, antioxidant activity with phytochemical screening of methanolic extract of Neolamarckia cadamba fruits. J. Med. Plant Res., 9: 151-158.
Sumi, S.A., M.A. Siraj, A. Hossain, M.S. Mia, S. Afrin and M.M. Rahman, 2016. Investigation of the key pharmacological activities of Ficus racemosa and analysis of its major bioactive polyphenols by HPLC-DAD. Evidence-Based Complement. Altern. Med., Vol. 2016.
Hossain, M.A. and M.D. Shah, 2015. A study on the total phenols content and antioxidant activity of essential oil and different solvent extracts of endemic plant Merremia borneensis. Arabian J. Chem., 8: 66-71.
Shinde, U.A., A.S. Phadke, A.M. Nair, A.A. Mungantiwar, V.J. Dikshit and M.N. Saraf, 1999. Membrane stabilizing activity-a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia, 70: 251-257.
Ahmed, F., M.S. Selim, A.K. Das and M.S. Choudhuri, 2004. Anti-inflammatory and antinociceptive activities of Lippia nodiflora Linn., Pharmazie, 59: 329-330.
Sahu, M.C. and R.N. Padhy, 2013. In vitro antibacterial potency of Butea monosperma Lam. against 12 clinically isolated multidrug resistant bacteria. Asian Pac. J. Trop. Dis., 3: 217-226.
Katsube, T., M. Yamasaki, K. Shiwaku, T. Ishijima, I. Matsumoto, K. Abe and Y. Yamasaki, 2010. Effect of flavonol glycoside in mulberry (Morus alba L.) leaf on glucose metabolism and oxidative stress in liver in dietâ€induced obese mice. J. Sci. Food. Agric., 90: 2386-2392.
Prakash, O., R. Kumar, A. Mishra and R. Gupta, 2009. Artocarpus heterophyllus (Jackfruit): An overview. Pharmacogn. Rev., 3: 353-358.
Morshed, M.A., A. Uddin, R. Saifur, A. Barua and A. Haque, 2011. Evaluation of antimicrobial and cytotoxic properties of Leucas aspera and Spilanthes paniculata. Int. J. Biosci., 1: 7-16.
Elmastas, M., O. Isildak, I. Turkekul and N. Temur, 2007. Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. J. Food Compos. Anal., 20: 337-345.
Wang, K.J., C.R. Yang and Y.J. Zhang, 2007. Phenolic antioxidants from Chinese toon (fresh young leaves and shoots of Toona sinensis). Food Chem., 101: 365-371.
Madaan, R., G. Bansal, S. Kumar and A. Sharma, 2011. Estimation of total phenols and flavonoids in extracts of Actaea spicata roots and antioxidant activity studies. Indian J. Pharm. Sci., 73: 666-669.
Henriquez, C., S. Almonacid, I. Chiffelle, T. Valenzuela and M. Araya et al., 2010. Determination of antioxidant capacity, total phenolic content and mineral composition of different fruit tissue of five apple cultivars grown in chile. Chilean Agric. Res. J., 70: 523-536.
Yang, C.M. and J.B. Russell, 1992. Resistance of proline-containing peptides to ruminal degradation In vitro. Applied Environ. Microbiol., 58: 3954-3958.
Hossain, H., A.F.M. Shahid-Ud-Daula, I.A. Jahan, I. Nimmi, K. Hasan and M.M. Haq, 2012. Evaluation of antinociceptive and antioxidant potential from the leaves of Spilanthes paniculata growing in Bangladesh. Int. J. Pharm. Phytopharmacol. Res., 1: 178-186.
Umukoro, S. and R.B. Ashorobi, 2006. Evaluation of anti-inflammatory and membrane stabilizing property of aqueous leaf extract of Momordica charantia in rats. Afr. J. Biomed. Res., 9: 119-124.
Saffoon, N., R. Uddin, N. Subhan, H. Hossain, H.M. Reza and M.A. Alam, 2014. In vitro anti-oxidant activity and HPLC-DAD system based phenolic content analysis of codiaeum variegatum found in Bangladesh. Adv. Pharm. Bull., 4: 533-541.
Richter, L.A., J. Salazar and E. Rodriguez, 2003. A phytochemical analysis to suggest new applications of anti-inflammatory plants from the Dominican Republic. Emanations, 4: 24-30.
Bauer, A.W., W.M.M. Kirby, J.C. Sherris and M. Turck, 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol., 45: 493-496.
Onasanwo, S.A. and R.A. Elegbe, 2006. Anti-nociceptive and anti-inflammatory properties of the leaf extracts of Hedranthera barteri in rats and mice. Afr. J. Biomed. Res., 9: 109-117.
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