Antioxidant Effects of Tomato Juice on Reducing Serum Malondialdehyde Levels in Menopausal Rats
DOI:
https://doi.org/10.3923/pjn.2020.362.366Keywords:
Menopause, rats, serum malondialdehyde levels, tomato juice, vitamin CAbstract
Background and Objective: Menopause is characterized by a loss in reproductive ability. Aging is a progressive loss of biochemical and physiological processes after the reproductive phase, which is affected by oxidative stress and free radicals. Tomatoes contain antioxidants. This study aimed to determine the antioxidant effects of tomato juice on serum malondialdehyde levels in menopausal rats. Materials and Methods: This was an experimental study. Twenty-four female Sprague-Dawley rats were divided into four groups. The negative control was a group of sham procedure rats that were treated with distilled water. The positive control was a group of bilateral ovariectomized rats that were treated with distilled water. The first treatment was a group of bilateral ovariectomized rats that were treated with tomato juice at the dosage of 11 g/200 g body weight day–1. The second treatment was a group of bilateral ovariectomized rats that were treated with tomato juice at the dosage of 15 g/200 g body weight day–1. Treatment was given for 28 days. Results: The highest serum malondialdehyde level was found in the positive control group (126.70±24.76 ng mL–1). The serum malondialdehyde level in the first treatment group (120.40±39.81 ng mL–1) was lower than that in the positive control group (126.70±24.76 ng mL–1). The lowest serum malondialdehyde level was in the second treatment group (81.33±10.51 ng mL–1). There was a negative correlation between the amount of tomato juice and the serum malondialdehyde level (b = -0.573). Conclusion: Tomato juice, as an antioxidant, can reduce serum malondialdehyde levels in menopausal rats. The higher the dose of tomato juice, the lower the serum malondialdehyde level.
References
Sturdee, D.W. and N. Panay, 2010. Recommendations for the management of postmenopausal vaginal atrophy. Climacteric, 13: 509-522.
Saimin, J., S. Wicaksono, J. Junuda and M. Minarti, 2018. Factors affecting the development of anxiety in postmenopausal women: a crosssectional study in coastal areas. Indones. J. Obstet. Gynecol., 6: 133-136.
Rahman, K., 2007. Studies on free radicals, antioxidants, and co-factors. Clin. Interv. Aging., 2: 219-236.
López-OtÃn, C., M.A. Blasco, L. Partridge, M. Serrano and G. Kroemer, 2013. The hallmarks of aging. Cell, 153: 1194-1217.
Liochev, S.I., 2015. Which is the most significant cause of aging? Antioxidants, 4: 793-810.
Tosato, M., V. Zamboni, A. Ferrini and M. Cesari, 2007. The aging process and potential interventions to extend life expectancy. Clin. Interv. Aging., 2: 401-412.
Ayala, A., M.F. Muñoz and S. Argüelles, 2014. Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid. Med. Cell. Longevity, Vol. 2014.
Droge, W., 2002. Free radicals in the physiological control of cell function. Phsyol. Rev., 82: 47-95.
Zalukhu, M.L., A.R. Phyma and R.T. Pinzon, 2016. Proses Menua, Stres Oksidatif, dan Peran Antioksidan. Pusat Penelitian Bioteknologi.
Sayuti, K. and R. Yenrina, 2015. Antioksidan alami dan sintetik / Kesuma Sayuti, Rina Yenrina. Andalas University Press Indonesia.
Canene-Adams, K., J.K. Campbell, S. Zaripheh, E.H. Jeffery and J.W. Erdman, 2005. The tomato as a functional food. J. Nutr., 135: 1226-1230.
Andayani, R., Maimunah and Y. Lisawati, 2008. Penentuan aktivitas antioksidan, kadar fenolat total dan likopen pada buah tomat (Solanum lycopersicum L.). J. Sains Teknologi Farmasi, 13: 31-37.
Maong, R., J.A. Rorong and F. Fatimah, 2016. Aktivitas ekstrak buah tomat (Lycopersicum esculentum Mill) sebagai penstabil oksigen singlet dalam reaksi fotooksidasi asam linoleat. J. Mipa Unsrat Online, 5: 60-64.
Mu’nisa, A., 2012. Analisis kadar likopen dan uji aktivitas antioksidan pada tomat asal sulawesi selatan. J. Bionature, 13: 62-66.
Kailaku, S.I., K.T. Dewandari and Sunarmani, 2007. Potensi likopen dalam tomat untuk kesehatan. Buletin Teknologi Pascapanen Pertanian, 3: 50-58.
Mendelová, A., M. Fikselová and Ľ. Mendel, 2013. Carotenoids and lycopene content in fresh and dried tomato fruits and tomato juice. Acta Universitatis Agric. Silviculturae Mendelianae Brunensis, 61: 1329-1337.
Ma’sum, J., Isnaeni, R. Primaharinastiti and F. Annuryanti, 2014. Perbandingan aktivitas antioksidan ekstrak aseton tomat segar dan pasta tomat terhadap 1,1-diphenyl-2-picrylhidrazyl (DPPH). J. Farmasi Ilmu Kefarmasian Indonesia, 1: 59-62.
Prasetya, A.W. and Y. Widjayanti, 2015. Effect of tomato (Lycopersicum commune) juice on vaginal wall elasticityin menopausal rats. Folia Med. Indonesiana, 51: 132-136.
Grady, D., 2006. Management of menopausal symptoms. N. Engl. J. Med., 355: 2338-2347.
Tinelli, A., A. Malvasi, S. Rahimi, R. Negro and D. Vergara et al., 2010. Age-related pelvic floor modifications and prolapse risk factors in postmenopausal women. Menopause 17: 204-212.
May, J.M. and Z.C.Qu, 2005. Transport and intracellular accumulation of vitamin C in endothelial cells: relevance to collagen synthesis. Arch. Biochem. Biophys., 434: 178-186.
Pinnell, S.R., 2003. Cutaneous photodamage, oxidative stress, and topical antioxidant protection. J. Am. Acad. Dermatol., 48: 1-22.
Wahyono, P., 2017. Efek ekstrak buah tomat (Licopersicum pyriforme) terhadap eksp kolagen tipe 1, MMP-1 dan MMP-3 pada penuaan kulit. J. Kedokteran Brawijaya, Vol. 24.
Saimin, J., H. Hendarto and Soetjipto, 2020. The effect of tomato juice on the expression of matrix metalloproteinase-2 (MMP-2) and type-1 collagen on the vaginal wall of the menopausal rats. Bali. Med. J. 8: 707-712.
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