Lemon Peels (Citrus latifolia, Rutaceae) Compared to Garlic Cloves (Allium Sativum L.) has the Highest Antioxidant Activity "In vitro" and Protect Against Free Radical Attack in Streptozotocin-induced Diabetic
Authors
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Khelladi Hadj Mostefa
Department of Biology, Faculty of Natural Sciences and Life, University Mustapha Stambouli, Mascara, Algeria
- Krouf Djamil Laboratory of Clinical and Metabolic Nutrition, Department of Biology, Faculty of Natural Sciences and Life, University Oran 1, Oran, Algeria
- Dida-Taleb Nawal Laboratory of Clinical and Metabolic Nutrition, Department of Biology, Faculty of Natural Sciences and Life, University Oran 1, Oran, Algeria
- Guenzet Akila Laboratory of Clinical and Metabolic Nutrition, Department of Biology, Faculty of Natural Sciences and Life, University Oran 1, Oran, Algeria
- Mir Hakima Faculty of Medicine, University of Abdelhamid Ibn Badis Mostaganem, Algeria
DOI:
https://doi.org/10.3923/pjn.2024.30.38Keywords:
Antioxidant activity, diabetes, garlic cloves, lemon peels, phenolic compounds, ratsAbstract
Background and Objective: Lemon and garlic have been used for a long time in traditional medicine. This “in vitro” study aimed to screen hydro-methanolic extract (HME) of garlic gloves (gc) and lemon peels (lp) and evaluate their effect on oxidative stress, lipid profile and glycaemia in diabetic rats. Materials and Methods: After preparation HME of two plants and performs a phytochemical screening, 24 male wistar rats were divided in four groups. Control group (C) received citrate buffer intraperitoneal. The other groups received Streptozotocin (40 mg kg–1 BW, ip) injection to induce diabetes. One diabetic group (D) was untreated, the two other groups were treated by lp extract (D-lp) or gc extract (D-gc) (200 mg kg-1 BW Day-1). Results: The "in vitro" study showed that lpHME compared to gcHME were richer in total polyphenols, flavonoids and condensed tannins and has a better DPPH radical scavenging action and anti-hemolytic potential. The "in vivo" study showed that in D-gc compared to D-lp and D groups, body weight was increased significantly and intraperitoneal glucose tolerance was improved. At Day 28, glycaemia was reduced significantly in D-gc and D-lp compared to D group. In diabetic rats, lp decreased total cholesterol, LDL-cholesterol and triacylglycerols. Antioxidant study showed that TBARS levels were significantly reduced in D-lp and D-gc groups in kidney. Therefore, Kidney glutathione values increased in D-gc compared to D-lp groups. Conclusion: Results suggested that feeding lp improved redox status, lipid profile and glycaemia. Also, gc decreased glycaemia, glucose tolerance and antioxidant defense. Both plants reduced diabetes complications by different mechanisms of action.
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