Research Article Open Access

Lemon Peels (Citrus latifolia, Rutaceae) Compared to Garlic Cloves (Allium Sativum L.) has the Highest Antioxidant Activity "In vitro" and Protect Against Free Radical Attack in Streptozotocin-induced Diabetic

Authors

CORRESPONDING AUTHOR
Khelladi Hadj Mostefa
iD 0000-0002-0092-4591
Department of Biology, Faculty of Natural Sciences and Life, University Mustapha Stambouli, Mascara, Algeria
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Krouf Djamil
Laboratory of Clinical and Metabolic Nutrition, Department of Biology, Faculty of Natural Sciences and Life, University Oran 1, Oran, Algeria
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Dida-Taleb Nawal
Laboratory of Clinical and Metabolic Nutrition, Department of Biology, Faculty of Natural Sciences and Life, University Oran 1, Oran, Algeria
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Guenzet Akila
Laboratory of Clinical and Metabolic Nutrition, Department of Biology, Faculty of Natural Sciences and Life, University Oran 1, Oran, Algeria
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Mir Hakima
Faculty of Medicine, University of Abdelhamid Ibn Badis Mostaganem, Algeria
Published on: May 02, 2024
DOI 10.3923/pjn.2024.30.38
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Abstract

Background and Objective: Lemon and garlic have been used for a long time in traditional medicine. This “in vitro” study aimed to screen hydro-methanolic extract (HME) of garlic gloves (gc) and lemon peels (lp) and evaluate their effect on oxidative stress, lipid profile and glycaemia in diabetic rats. Materials and Methods: After preparation HME of two plants and performs a phytochemical screening, 24 male wistar rats were divided in four groups. Control group (C) received citrate buffer intraperitoneal. The other groups received Streptozotocin (40 mg kg–1 BW, ip) injection to induce diabetes. One diabetic group (D) was untreated, the two other groups were treated by lp extract (D-lp) or gc extract (D-gc) (200 mg kg-1 BW Day-1). Results: The "in vitro" study showed that lpHME compared to gcHME were richer in total polyphenols, flavonoids and condensed tannins and has a better DPPH radical scavenging action and anti-hemolytic potential. The "in vivo" study showed that in D-gc compared to D-lp and D groups, body weight was increased significantly and intraperitoneal glucose tolerance was improved. At Day 28, glycaemia was reduced significantly in D-gc and D-lp compared to D group. In diabetic rats, lp decreased total cholesterol, LDL-cholesterol and triacylglycerols. Antioxidant study showed that TBARS levels were significantly reduced in D-lp and D-gc groups in kidney. Therefore, Kidney glutathione values increased in D-gc compared to D-lp groups. Conclusion: Results suggested that feeding lp improved redox status, lipid profile and glycaemia. Also, gc decreased glycaemia, glucose tolerance and antioxidant defense. Both plants reduced diabetes complications by different mechanisms of action.

Keywords:

Antioxidant activity, diabetes, garlic cloves, lemon peels, phenolic compounds, rats

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Mostefa, K. H., Djamil, K., Nawal, D.-T., Akila, G., & Hakima, M. (2024). Lemon Peels (Citrus latifolia, Rutaceae) Compared to Garlic Cloves (Allium Sativum L.) has the Highest Antioxidant Activity "In vitro" and Protect Against Free Radical Attack in Streptozotocin-induced Diabetic . Pakistan Journal of Nutrition, 23, 30–38. https://doi.org/10.3923/pjn.2024.30.38
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