Effect of Gari Diet on Marker Enzymes of Mice Liver Mitochondria


Authors

  • I.N. Nwachukwu Department of Microbiology, Federal University of Technology, P.M. B. 1526, Owerri, Nigeria
  • O. Obidua Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
  • E.U. Ezeji Department of Biotechnology, University of Nigeria, Nsukka, Nigeria
  • I.G. Kalu Department of Biotechnology, University of Nigeria, Nsukka, Nigeria

DOI:

https://doi.org/10.3923/pjn.2009.414.418

Keywords:

Cassava, cyanide, enzyme activity, inhibition

Abstract

Male and female albino mice were fed on maize (control) and gari (a dried cassava product) based diets for 5 weeks. Liver mitochondrial fractions were assayed for succinate dehydrogenase and Cytochrome C oxidase activities. There was an overall reduction in the activity of the two enzymes studied throughout the experiment. The reduction in activity was pronounced in Cytochrome c oxidase. Kinetic studies of succinate dehydrogenase showed an apparent increase in the Km of the gari fed animals-further supporting that inhibition took place. There was also a reduction in the mitochondrial respiratory control ratio (RCR) for the gari fed mice. The percentage reduction in RCR increased from 4.4% in the 2nd week to about 45% in the 5th week.

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Published

15.03.2009

Issue

Vol. 8 No. 4 (2009)

Section

Research Article

License

Copyright (c) 2009 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Nwachukwu, I., Obidua, O., Ezeji, E., & Kalu, I. (2009). Effect of Gari Diet on Marker Enzymes of Mice Liver Mitochondria. Pakistan Journal of Nutrition, 8(4), 414–418. https://doi.org/10.3923/pjn.2009.414.418