Effect of Gari Diet on Marker Enzymes of Mice Liver Mitochondria
Abstract
Male and female albino mice were fed on maize (control) and gari (a dried cassava product) based diets for 5 weeks. Liver mitochondrial fractions were assayed for succinate dehydrogenase and Cytochrome C oxidase activities. There was an overall reduction in the activity of the two enzymes studied throughout the experiment. The reduction in activity was pronounced in Cytochrome c oxidase. Kinetic studies of succinate dehydrogenase showed an apparent increase in the Km of the gari fed animals-further supporting that inhibition took place. There was also a reduction in the mitochondrial respiratory control ratio (RCR) for the gari fed mice. The percentage reduction in RCR increased from 4.4% in the 2nd week to about 45% in the 5th week.
Keywords:
Cassava, cyanide, enzyme activity, inhibitionArticle Metrics
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