Evaluation Effects of Clove Methanol Extract on Methane Production in the in vitro Condition

Authors

  • Ali Halimi Shabestari Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
  • Ramin Salamatdoustnobar Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
  • Naser Maheri-Sis Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
  • Abolfazl Gorbani Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
  • Kamal Mirzadeh Ahari Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
  • Ali Noshadi Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
  • Hossien Samadi National Iranian Gas Company, East Azerbaijan Province, Shabestar Center, Iran
  • Jafar Salimi Nezhad National Iranian Gas Company, East Azerbaijan Province, Shabestar Center, Iran

DOI:

https://doi.org/10.3923/pjn.2011.1154.1157

Keywords:

Clove, methane production, methanol extract

Abstract

The aim of this study was to investigate effects of different levels of Clove Methanol Extract (CME) on methane production in ruminant with practical diets. Two experimental diets (diet1: 100% forage and diet 2: 100% barley) were used to investigate effects of 3 levels of CME (0, 0.5 and 1 ml) on methane production at 2, 4, 12, 16, 24, 48, 72 and 96 h of incubation. Gas production was continuously measured by EX-TEC HS 680 GC. Cumulative gas production was recorded at 2, 4, 12, 16, 24, 48, 72 and 96 h of incubation periods. Data processed in excel and analyzed using SAS statistical analyze software. Result show that Clove methanol extract in initial time of incubation (2 and 4 h) not affects on the decreased of methane production, but increase incubation time CME significantly decreased methane gas volume in two diets and all of CME levels and could significantly decrease methane volume in the treatment. According to result we could suggest CME for improve methane production in the in vitro condition and need further research for application CME in field of ruminant nutrition.

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Published

15.11.2011

Issue

Vol. 10 No. 12 (2011)

Section

Research Article

License

Copyright (c) 2011 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Shabestari, A. H., Salamatdoustnobar, R., Maheri-Sis, N., Gorbani, A., Ahari, K. M., Noshadi, A., Samadi, H., & Nezhad, J. S. (2011). Evaluation Effects of Clove Methanol Extract on Methane Production in the in vitro Condition. Pakistan Journal of Nutrition, 10(12), 1154–1157. https://doi.org/10.3923/pjn.2011.1154.1157

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