Bacillus subtilis Strain VITNJ1 Potential Probiotic Bacteria in the Gut of Tilapia (Oreochromis niloticus) are Cultured in Floating Net, Maninjau Lake, West Sumatra
DOI:
https://doi.org/10.3923/pjn.2014.710.715Keywords:
Bacillus subtilis, bacteria, probiotic, tilapia, west sumatraAbstract
Tilapia (Oreochromis niloticus) is one kind of fish are cultured in floating net (KJA) Maninjau lake, West Sumatra. This study was aimed to know about proteolytic bacteria found in the gut of fish Nila can be used as candidate probiotics. Materials used in this study were derived from Nila fish farmers in the vicinity of Maninjau lake. The research method used is the isolation, identification and characterization of bacteria. Identification was conducted on the morphological and biochemical characteristics of bacteria. It was found that 55 isolates of bacteria that are morphological and biochemical grouped into three namely genus are Bacillus, Achromobacter and Enterobacter. Based on the production of extracellular proteolytic enzymes known that isolates B.3.2 had the highest proteolitic index. Based on characteristics examination and PCR analysis the isolate B.3.2 was primarily identified as Bacillus subtilis strain VITNJ1 bacteria can be used as a candidate probiotic.
References
Abraham, T.J., S. Mondal and C.S. Babu, 2008. Effect of commercial aquaculture probiotic and fish gut antagonistic bacterial flora on the growth and disease resistance of ornamental fishes Carassius auratus and Xiphophorus helleri. J. Fish. Aquat. Sci., 25: 27-30.
Austin, B., 2002. The bacterial microflora of fish. Sci. World J., 2: 558-572.
Agrawal, R., 2005. Probiotics: An emerging food supplement with health benefits. Food Biotechnol., 19: 227-246.
Azevedo, P.A., C.L. Podemski, R.H. Hesslein, S.E.M. Kasian, D.L. Findlay and D.P. Bureau, 2011. Estimation of waste outputs by a rainbow trout cage farm using a nutritional approach and monitoring of lake water quality. Aquaculture, 311: 175-186.
Bairagi, A., K.S. Ghosh, S.K. Sen and A.K. Ray, 2002. Enzyme producing bacterial flora isolated from fish digestive tracts. Aquacult. Int., 10: 109-121.
Cowan, S.T., 1975. Cowan and Steel's Manual for the Identification of Medical Bacteria. Cambridge University Press, Cambridge, London.
Djide, N. and Sartini, 2008. Isolation, identification of Lactic Acid Bacteria (LAB) on cabbage (Brassica oleracea L.) and its potential as an antagonist of Vibrio harveyi. J. Mar. Sci. Fish., 18: 211-216.
Al-Faragi, J.K.H. and S.A.A. Alsaphar, 2012. Isolation and identification of Bacillus subtilus as (probiotic) from intestinal microflora of common carp Cyprinus carpio L. Proceeding of the 11th Veterinary Scientific Conference, (VSC'12), Baghdad, Iraq, pp: 355-361.
Fardiaz, S., 1989. Food microbiology laboratory practice guide. Faculty of Agricultural Technology, Department of Food Technology and Nutrition, Bogor Agricultural University, Bogor, Indonesia.
Feliatra, I.E. and E. Suryadi, 2004. Isolation and identification of probiotic bacteria from fish Kerapu Macan (Ephinephelus fuscogatus) in fish feed efficiency efforts. J. Nat. Indonesia, 6: 75-80.
Lara-Flores, M. and M.A. Olvera-Novoa, 2013. The use of lactic acid bacteria isolated from intestinal tract of Nile tilapia (Oreochromis niloticus), as growth promoters in fish fed low protein diets. Latin Am. J. Aquat. Res., 41: 490-497.
Gangasuresh, P. and M.R. Bharathi, 2014. Comparative diversity profiles of gastrointestinal micro flora or normal and sick Oreochromis mossambicus (Peters). Asian J. Pharm. Sci. Tech., 4: 91-96.
Geethanjali, S. and A. Subash, 2011. Optimization of protease production by Bacillus subtilis isolated from mid gut of fresh water fish Labeo rohita. World J. Fish Mar. Sci., 3: 88-95.
Gomez, R.G.D. and M.A. Shen, 2008. Influence of probiotics on the growth and digestive enzyme activity of white Pacific shrimp (Litopenaeus vannamei). J. Ocean Univ. China, 7: 215-218.
Guo, L. and Z. Li, 2003. Effects of nitrogen and phosphorus from fish cage-culture on the communities of a shallow lake in middle Yangtze River basin of China. Aquaculture, 226: 201-212.
Hadioetomo, R., 1985. Basic Microbiology Practice Basic Laboratory Techniques and Procedures. Gramedia, Jakarta, Indonesia.
Hiraishi, A., Y. Kamagata and K. Nakamura, 1995. Polymerase chain reaction amplification and restriction fragment length polymorphism analysis of 16S rRNA genes from methanogens. J. Ferment. Bioeng., 79: 523-529.
Holt, J.G., N.R. Kreig, P.H.A. Sneath, J.T. Staley and S.T. Williams, 1994. Bergey's Manual of Determinative Bacteriology. 9th Edn., Lippincott Williams and Wilkins, Baltimore, USA., ISBN-13: 9780683006032, Pages: 787.
Ibrahim, B.U., J. Baba and M.S. Sheshi, 2014. Isolation and identification of bacteria associated with fresh and smoked fish (Clarias gariepinus) in Minna Metropolis, Niger State, Nigeria. J. Applied Environ. Microbiol., 2: 81-85.
Lay, B.W., 1994. Microbial Analysis in the Laboratory. P.T. Raja Grafindo Persada, Jakarta, Indonesia.
Lewis, D.H., 1973. Predominant aerobic bacteria of fish and shellfish. Ph.D. Thesis, Department of Veterinary Microbiology, Texas A and M University, College Station, Texas, USA.
Moeljanto, 1992. Preservation and Processing of Fish. Penebar Swadaya, Jakarta, Indonesia.
Musefiu, T.A., E.B. Obuko and A.O. Bolarinwa, 2011. Isolation and identification of aerobic bacteria flora of the skin and stomach of wild and cultured Clarias gariepinus and Oreochromis niloticus from Ibadan, Southwest Nigeria. J. Applied Sci. Res., 7: 1047-1051.
Musikasang, H., A. Tani, A. H-kittikun and S. Maneerat, 2009. Probiotic potential of lactic acid bacteria isolated from chicken gastrointestinal digestive tract. World J. Microbiol. Biotechnol., 25: 1337-1345.
Ntengwe, F.W. and M.O. Edema, 2008. Physico-chemical and microbiological characteristics of water for fish production using small ponds. Phys. Chem. Earth, 33: 701-707.
Perdana, A.B., 2011. Study of genetic diversity of intestinal bacteria tilapia (Oreochromis niloticus) through technique metagenom based. Indonesia University, Indonesia.
Pitcher, D.G., N.A. Saunders and R.J. Owen, 1989. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett. Applied Microbiol., 8: 151-156.
Salminen, S., A.V. Wright and A. Ouwehand, 2004. Lactic Acid Bacteria. 1st Edn., Marcel Dekker Inc., New York.
Subagiyo and A. Djunaedi, 2011. Screening of candidate probiotic bacteria from the digestive tract groupers based anti-bacterial activity and production of extracellular proteolytic enzymes. J. Mar. Sci., 16: 41-48.
Syandri, H., 2013. The use of fish Nilem (Osteochilus haselti CV) and fish Tawes (Puntius javanicus CV) as a biocontrol agent Maninjau cleaner waters, West Sumatra. J. Nat. Indonesia, 6: 87-90.
Tangko, A.M., A. Mansyur and Reski, 2007. The use of probiotics in feed fish rearing milkfish in floating net cages in the sea. J. Aquat. Res., 2: 33-40.
Thillaimaharani, K.A., A.R. Logesh, K. Sharmila, B.K. Magdoom and M. Kalaiselvam, 2012. Studies on the intestinal bacterial flora of tilapia Oreochromis mossambicus (Peters, 1852) and optimization of alkaline protease by Virgibacillus pantothenticus. J. Microbiol. Antimicrob., 4: 79-87.
Trakroo, M.D. and R. Agarwal, 2011. Qualitative and quantitative study on bacterial flora of farm raised Rohu, Labeo rohita (Ham.) in India. J. Recent Trends Biosci., 1: 66-71.
Uddin, N. and A.H. Al-Harbi, 2012. Bacterial flora of polycultured common carp (Cyprinus carpio) and African catfish (Clarias gariepinus). Int. Aquat. Res., Vol. 4.
White, T.J., T. Bruns, S. Lee and J. Taylor, 1990. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, Innis, M.A., D.H. Gelfand, J.J. Sninsky and T.J. White (Eds.), Academic Press, Cambridge, Massachusetts, ISBN: 9780123721808, pp: 315-322.
Zapata, A.A. and M. Lara-Flores, 2013. Antimicrobial activities of lactic acid bacteria strains isolated from Nile Tilapia intestine (Oreochromis niloticus). J. Biol. Life Sci., 4: 164-171.
Ziaei-Nejad, S., M.H. Rezaei, G.A. Takami, D.L. Lovett, A.R. Mirvaghefi and M. Shakouri, 2006. The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. Aquaculture, 252: 516-524.
Zhou, X.X., Y.B. Wang and W.F. Li, 2009. Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality, survival rate and digestive enzyme activities. Aquaculture, 287: 349-353.
Mubarik, N.R., 2001. [Purification and characterization extracellular proteases bacterial isolates from thermophilic GP-04]. Ph.D Thesis, Institute of Agriculture Bogor, Indonesia.
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