Survey of Starch Amylose Content in Naked Barley (H. vulgare. Nudum)

Abstract

Starch of two lines of naked barley were extracted by three different methods of washing. The apparent amylose content were determined with the same standard method which recently has been modified for use with samples of 2-3 seeds of cereal. In the present experiment factorial design (2x3x3) was used. The starch extracted by soaking the seeds overnight in dilute ammonia solution grinding in NaCl solution in a microfuge tube with an appropriate pestle and decanting the starch slurry. Then it was washed in 4M NaCl, SDS and acetone. The results of apparent amylose content of this method statistically were better than the two others. With present selected method the apparent amylose content ranged from 18 to 34% was determined for 145 accessions of naked barley. The lowest amylose content was related to the line of 4062 from Pakistan and the highest one was related to the line of 743 from Turkmenstan. This range is considered sufficiently broad to allow amylose content to be further diversified through the working with more lines and breeding.