Influence of Fortified Tempe with Iron and Vitamin A to Increase Hemoglobin Level of Rats with Iron Deficiency Anemia
DOI:
https://doi.org/10.3923/pjn.2017.90.95Keywords:
Hemoglobin level, iron, tempe fortification, vitamin AAbstract
Objective: The study was conducted to assess the influence of fortified tempe with iron and vitamin A to increase hemoglobin level. Methodology: An experimental study of randomized pre test, post test and control group design was conducted on 30 Sprague-Dawley rats with iron deficiency anemia. Samples were divided into 6 groups randomly and then the rats were treated for 6 weeks. The treatments were as follows: (1) Standard feed (SF), (2) SF+TWF, (3) SF+TFe230, (4) SF+TFe271, (5) SF+TFe271+VA15 and (6) SF+TFe271+VA50, which TWF was tempe without fortification, TFe230 and TFe271 was tempe fortified with iron 230 and 271 ppm, VA15 was 15 ppm vitamin A and VA50 was 50 ppm vitamin A. Iron used was ferrous sulfate heptahydrate (FeSO4·7H2O) and vitamin A used was retinyl acetate. Results: Average hemoglobin level at baseline was 12.0±0.3 g dL–1. After a period of depletion, it decreased to 5.7±0.2 g dL–1. Average hemoglobin levels after treatment in the control rats (rats fed SF) was 11.6±0.2 g dL–1, rats that was given tempe fortified with iron had not reached the average level of 12.0 g dL–1 while rats that were given tempe fortified with iron+vitamin A SF+TFe271+VA15 and SF+TFe271+VA50 the average of hemoglobin levels were, respectively 12.2±0.1 and 12.4±0.2 g dL–1. ANCOVA test results showed that tempe fortified with iron and vitamin A significantly (p = 0.001) improved the level of hemoglobin (Hb). The highest average hemoglobin level was in the treatment group iron 271 ppm+vitamin A of 50 ppm. Conclusion: Iron and vitamin A fortification in tempe with ferrous sulfate heptahydrate (FeSO4·7H2O) 271 ppm and retinyl acetate 50 ppm may increase hemoglobin level in iron deficiency anemia rats.
References
WHO., 2001. Iron Deficiency Anaemia: Assessment, Prevention and Control. A Guide for Programme Managers. World Health Organization, Geneva.
Soemantri, A.G., 1989. Preliminary findings on iron supplementation and learning achievement of rural Indonesian children. Am. J. Clin. Nutr., 50: 698-701.
Lozoff, B., E. Jimenez and A.W. Wolf, 1991. Long-term developmental outcome of infants with iron deficiency. N. Engl. J. Med., 325: 687-694.
McLean, E., M. Cogswell, I. Egli, D. Wojdyla and B. de Benoist, 2007. Worldwide Prevalence of Anemia in Preschool Aged Children, Pregnant Women and Non-Pregnant Women of Reproductive Age. In: Nutritional Anemia, Kraemer, K. and M.B. Zimmerman (Eds.)., Sight and Life Press, Basel, Switzerland, pp: 1-12.
Husaini, M.A., D. Karyadi and H. Gunadi, 2001. Evaluation of Nutritional Anaemia Intervention Among Anemic Female Workers on a Tea Plantation. World Health Organization. Iron deficiency Anaemia. Assessment. Prevention and control. A guide for programme managers. WHO/NHD/01.3. Genewa.
Ekiz, C., L. Agaoglu, Z. Karakas, N. Gurel and I. Yalcin, 2005. The effect of iron deficiency anemia on the function of the immune system. Hematol. J., 5: 579-583.
Brabin, L. and B.J. Brabin, 1992. The cost of successful adolescent growth and development in girls in relation to iron and vitamin A status. Am. J. Clin. Nutr., 55: 955-958.
Astuti, R. and E. Handarsari, 2009. Usia menarche, indeks masa tubuh, frekuensi konsumsi dan status sosial ekonomi orang tua pada siswi SLTP di pinggir kota dan pusat kota, kota semarang. Semarang: Fakultas Kesehatan Masyarakat Universitas Muhammadiyah Semarang, Indonesia.
Alawi, F., 2003. Faktor-faktor yang berhubungan dengan konsumsi tempe pada remaja di SMUN 1 slawi dan SMUN 1 tegal. Skripsi, Jurusan Gizi Masyarakat dan Sumberdaya Keluarga, Fakultas Pertanian, Institut Pertanian Bogor, Bogor.
Dijkhuizen, M.A., F.T. Wieringa, C.E. West and M. Muherdiyantiningsih, 2001. Concurrent micronutrient deficiencies in lactating mothers and their infants in Indonesia. Am. J. Clin. Nutr., 73: 786-791.
Van Stuijvenberg, M.E., M. Kruger, C.J. Badenhorst, E.P. Mansvelt and J.A. Laubscher, 1997. Response to an iron fortification programme in relation to vitamin A status in 6-12-year-old school children. Int. J. Food Sci. Nutr., 48: 41-49.
Zimmermann, M.B., 2007. Interactions between Iron and Vitamin A, Riboflavin, Copper and Zinc in the Etiology Anemia. In: Nutritional Anemia, Kraemer, K. and M.B. Zimmerman (Eds.)., Sight and Life Press, Basel, Switzerland, pp: 199-213.
Tawali, A.B., 2000. Fortifikasi zat besi pada ragi tempe dan dan analisis ketersediaan (availability) zat besi pada tempe yang dihasilkan (Suatu kajian fortifikasi mikronutrien pada makanan tradisional). Seminar Makanan Tradisional, Pusat Kajian Makanan Tradisional (PKMT) Universitas Brawijaya, Malang.
Astuti, R., A. Syamsianah and S. Aminah, 2012. Tempe fortifikasi untuk penanggulangan anemia gizi besi pada Remaja. Laporan Hibah Bersaing Tahun I. Universitas Muhammadiyah Semarang, Indonesia.
Philip, G.R, H.N. Forrest and C.F. George Jr., 1993. AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition ad hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet. J. Nutr., 123: 1939-1951.
Naruki, S., M. Astuti, Y. Marsono and S. Raharjo, 2010. Sifat prooksidatif fortifikan nafeedta, dengan kecap kedelai manis sebagai makanan pembawa, dalam system biologis (Tikus). Majalah Ilmiah Agritech., 30: 244-249.
WHO. and CDC., 2007. Assessing the iron status of population. Including literature reviews, Report of a joint World Health Organization/ Centers for Disease Control and Prevention Technical Consultation on the assessment of iron status at the population level, Geneva, Switzerland, 6-8 April 2004.
Hill, P.G., 1985. The measurement of albumin in serum and plasma. Ann. Clin. Biochem., 22: 565-578.
Sheskin, D., 2004. Handbook of Parametric and Nonparametric Statistical Procedures. 3rd Ed., Chapman and Hall /CRC, Boca Raton, ISBN: 9781584884408, Pages: 1193.
Kamei, A., Y. Watanabe, T. Ishijima, M. Uehara and S. Arai et al., 2010. Dietary iron-deficient anemia induces a variety of metabolic changes and even apoptosis in rat liver: A DNA microarray study. Physiol. Genom., 42: 149-156.
Hoffbrand, V.A. and A. Mehta, 2008. At a Glance Hematologi. Erlangga, Jakarta.
Gropper, S.S., J.L. Smith and J.L. Groff, 2009. Advanced Nutrition and Human Metabolism. 5th Edn., Cengange Learning, Wadsworth, USA.
Hatefi, Y., 1985. The mitochondrial electron transport and oxidative phosphorylation system. Annu. Rev. Biochem., 54: 1015-1069.
Ajioka, R.S., J.D. Phillips and J.P. Kushner, 2006. Biosynthesis of heme in mammals. Biochim. Biophys. Acta (BBA)-Mol. Cell Res., 1763: 723-736.
Hesketh, J.E., M.H. Hesketh and G. Bermano, 1998. Regulatory signals in messenger RNA: Determinants of nutrient-gene interaction and metabolic compartmentation. Br. J. Nutr., 80: 307-321.
Olson, J.A., 1993. Atwater lecture: The irresistible fascination of carotenoids and vitamin A. Am. J. Clin. Nutr., 57: 833-839.
Fishman, S.M., P. Christian and K.P. West, 2000. The role of vitamins in the prevention and control of anaemia. Public Health Nutr., 3: 125-150.
Semba, R.D. and M.W. Bloem, 2002. The anemia of vitamin A deficiency: Epidemiology and pathogenesis. Eur. J. Clin. Nutr., 56: 271-281.
WHO., 2006. Guidelines on Food Fortification with Micronutrients. World Health Organization and Agriculture Organization of the United Nations, Geneva, Switzerland.
Haschke, F., E.E. Ziegler, B.B. Edwards and S.J. Fomon, 1986. Effect of iron fortification of infant formula on trace mineral absorption. J. Pediatr. Gastroenterol. Nutr., 5: 768-773.
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