Effect of Cations and Chemicals on the Activity of Partially Purified Cellulase from Tomato (Lycopersicon esculentum Mill) Fruits Deteriorated by Aspergillus flavus Linn
DOI:
https://doi.org/10.3923/pjn.2007.198.200Keywords:
Aspergillus flavus, cellulolytic activity, chromatography, ion-exchange, tomato fruitsAbstract
Within ten days of incubation, freshly ripe tomato fruits (Ibadan local variety) obtained from a local market in Ile-Ife, Osun State, Nigeria had completely deteriorated and proteins which possessed cellulolytic activity were released. The enzyme was partially purified by a combination of ammonium sulphate precipitation, molecular exclusion chromatography and ion-exchange chromatography. The enzyme was stimulated to varying degrees by Ca ++ , Mg ++ , Na + and K + but was inhibited by ethylene diamine tetraacetic acid, 2, 4-dinitrophenol and mercuric chloride.
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