Effect of Dietary Vegetable Oil Supplementation on C18 Fatty Acids and Conjugated Linoleic Acid Production; An In vitro Fermentation Study

Abstract

An in vitro rumen fermentation trial was conducted to investigate effect of dietary vegetable oil supplement on fatty acids metabolism in cattle. The incubation was carried out using rumen fluid obtained from Brahman x Native crossbred beef cattle. The diets were formulated by using basal diet with no vegetable oil (CON), basal diet with 4% of palm oil (PO), 4% of canola oil (CA), 4% of coconut oil (CO) and 4% of sunflower oil (SF). Fatty acid profiles were determined at 12, 24 and 48 h after incubation time. The results found that linoleic acid (LA) was decreased with diet CON, diet PO, diet CA and diet SF (p<0.05). Stearic acid (SA) resulted increased with diet CON, diet CA, diet CO and diet SF at 24 and 48 h. The SF diet contain high level of LA showed the highest concentration of cis9 trans11 conjugated linoleic acid (CLA) when compared with other diets. Vaccenic acid (VA) was increased with all the five diet, especially with diet SF and diet CA after 24 h. Base on this study, it suggests that supplementation of SF could improve the concentration of VA and cis9 trans11 CLA in the rumen.