Research Article Open Access

Preventive Effect of Corosolic Acid on Lipid Profile Against Carbon Tetrachloride-Induced Hepatotoxic Rats

Authors

CORRESPONDING AUTHOR
Abdullah H. Al-Assaf
Department of Food Science and Nutrition, College of Food and Agricultural Science,King Saud University, P.O. Box 2460, Riyadh-11451, Saudi Arabia
Published on: July 15, 2013
DOI 10.3923/pjn.2013.748.752
PDF

Abstract

The present study was designed to investigate the hepatoprotective activity of corosolic acid in CCl4-induced hepatotoxic male albino rats and included an assessment of the antihyperlipidemic properties. Liver necrosis was induced by intraperitoneal injection of CCl4 (1.25 mL/kg b.wt.). Hyperlipidemic agents, such as lipid peroxidation, lipid profile and liver function markers were assessed. The activities of the hepatic marker enzymes such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma glutamyl transpeptidase (GGT) were increased significantly in CCl4-treated animals. In the serum, increased levels of very low density lipoprotein cholesterol (VLDL-C) and low density lipoprotein cholesterol (LDL-C) and decreased high density lipoprotein cholesterol (HDL-C) were observed. Further, an increase in the levels of total cholesterol (TC), phospholipids (PL), triglycerides (TG) and free fatty acids (FFA) in serum and liver were observed in hepatotoxic rats, whereas these hepatic marker enzymes and lipid profiles were significantly protected in corosolic acid pretreated on CCl4-treated animals. These results indicate that the corosolic acid can potentially ameliorate the lipids abnormalities and protect hepatic damage against CCl4-induced hepatotoxicity rats.

Keywords:

CCL4, corosolic acid, lipid profile, liver, silymarin

Article Metrics

107 Abstract Views
142 File Views
0 Downloads

License

Copyright (c) 2013 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

Article Citation Metrics

Find Preventive Effect of Corosolic Acid on Lipid Profile Against Carbon Tetrachloride-Induced Hepatotoxic Rats on PlumX

How to Cite

Al-Assaf, A. H. (2013). Preventive Effect of Corosolic Acid on Lipid Profile Against Carbon Tetrachloride-Induced Hepatotoxic Rats. Pakistan Journal of Nutrition, 12(8), 748–752. https://doi.org/10.3923/pjn.2013.748.752
Crossref
Scopus
Google Scholar
Europe PMC

References

1.
Agellon, L.B., A. Walsh, T. Hayek, P. Moulin and X.C. Jiang et al., 1991. Reduced high density lipoprotein cholesterol in human cholesteryl ester transfer protein transgenic mice. J. Biol. Chem., 266: 10796-10801.
2.
Agency for Toxic Substances and Disease Registry, 2005. Toxicological profile for carbon tetrachloride. US Department of Health and Human Services, Atlanta, USA.
3.
Allain, C.C., L.S. Poon, C.S.G. Chan, W. Richmond and P.C. Fu, 1974. Enzymatic determination of total serum cholesterol. Clin. Chem., 20: 470-475.
4.
Bilgin, H.M., M. Atmaca, B.D. Obay, S. Ozekinci, E. Tasdemir and A. Ketani, 2011. Protective effects of coumarin and coumarin derivatives against carbon tetrachloride-induced acute hepatotoxicity in rats. Exp. Toxicol. Pathol., 63: 325-330.
5.
Brinton, E.A., S. Eisenberg and J.L. Breslow, 1990. A low-fat diet decreases High Density Lipoprotein (HDL) cholesterol levels by decreasing HDL apolipoprotein transport rates. J. Clin. Invest., 85: 144-151.
6.
Caligiani, A., G. Malavasi, G. Palla, A. Marseglia, M. Tognolini and R. Bruni, 2013. A simple GC-MS method for the screening of betulinic, corosolic, maslinic, oleanolic and ursolic acid contents in commercial botanicals used as food supplement ingredients. Food Chem., 136: 735-741.
7.
Murthy, K.N.C. and S. Manchali, 2012. Anti-diabetic Potentials of Red Beet Pigments and other Constituents. In: Red Beet Biotechnology: Food and Pharmaceutical Applications, Neelwarne, B. (Ed.). Springer, USA., ISBN-13: 9781461434573, pp: 155-174.
8.
Das, J., J. Ghosh, A. Roy and P.C. Sil, 2012. Mangiferin exerts hepatoprotective activity against D-galactosamine induced acute toxicity and oxidative/nitrosative stress via Nrf2-NFκB pathways. Toxicol. Appl. Pharmacol., 260: 35-47.
9.
Deng, J.S., Y.C. Chang, C.L. Wen, J.C. Liao and W.C. Hou et al., 2012. Hepatoprotective effect of the ethanol extract of Vitis thunbergii on carbon tetrachloride-induced acute hepatotoxicity in rats through anti-oxidative activities. J. Ethnopharmacol., 142: 795-803.
10.
EPA, 2003. Water quality guidance for the Great Lakes system: Pollutants of initial focus in the Great Lakes water quality initiative. U.S. Environmental Protection Agency, 40 CFR Part 132, Washington, DC., USA., June 6, 2003.
11.
Falholt, K., B. Lund and W. Falholt, 1973. An easy colorimetric micromethod for routine determination of free fatty acids in plasma. Clinica Chimica Acta, 46: 105-111.
12.
Friedewald, W.T., R.I. Levy and D.S. Fredrickson, 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem., 18: 499-502.
13.
Izzo, C., F. Grillo and E. Murador, 1981. Improved method for the determination of high density lipoprotein cholesterol I. Isolation of high-density lipoproteins by the use of polyethylene glycol 6000. Clin. Chem., 27: 371-374.
14.
Kind, P.R.N. and E.J. King, 1954. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J. Clin. Pathol., 7: 322-326.
15.
Ma, L., D. Gan, M. Wang, Z. Zhang, C. Jiang and X. Zeng, 2012. Optimization of extraction, preliminary characterization and hepatoprotective effects of polysaccharides from Stachys floridana Schuttl. ex Benth. Carbohydr. Polym., 87: 1390-1398.
16.
Manibusan, M.K., M. Odin and D.A. Eastmond, 2007. Postulated carbon tetrachloride mode of action: A review. J. Environ. Sci. Health C, 25: 185-209.
17.
McGowan, M.W., J.D. Artiss, D.R. Strandbergh and B. Zak, 1983. A peroxidase-coupled method for the colorimetric determination of serum triglycerides. Clin. Chem., 29: 538-542.
18.
Pushpavalli, G., C. Veeramani and K.V. Pugalendi, 2010. Influence of chrysin on hepatic marker enzymes and lipid profile against D-galactosamine-induced hepatotoxicity rats. Food Chem. Toxicol., 48: 1654-1659.
19.
Ravikumar, S. and M. Gnanadesigan, 2012. Hepatoprotective and antioxidant properties of Rhizophora mucronata mangrove plant in CCl4 intoxicated rats. J. Exp. Clin. Med., 4: 66-72.
20.
Reitman, S. and S. Frankel, 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol., 28: 56-63.
21.
Rosalki, S.B. and D. Rau, 1972. Serum γ-glutamyl transpeptidase activity in alcoholism. Clin. Chim. Acta., 39: 41-47.
22.
Sabesin, S.M., L.B. Kuiken and J.B. Ragland, 1975. Lipoprotein and lecithin: Cholesterol acyltransferase changes in galactosamine-induced rat liver injury. Science, 190: 1302-1304.
23.
Ebenezar, K.K., V. Sathish and T. Devaki, 2003. Effect of L-arginine and L-lysine on lysosomal hydrolases and membrane bound phosphatases in experimentally induced myocardial infarction in rats. Mol. Cell. Biochem., 247: 163-169.
24.
Shen, X., Y. Tang, R. Yang, L. Yu, T. Fang and J.A. Duan, 2009. The protective effect of Zizyphus jujube fruit on carbon tetrachloride-induced hepatic injury in mice by anti-oxidative activities. J. Ethanopharmacol., 122: 555-560.
25.
Shim, J.Y., M.H. Kim, H.D. Kim, J.Y. Ahn, Y.S. Yun and J.Y. Song, 2010. Protective action of the immunomodulator ginsan against carbon tetrachloride-induced liver injury via control of oxidative stress and the inflammatory response. Toxicol. Applied Pharmacol., 242: 318-325.
26.
Singhal, K.G. and G.D. Gupta, 2012. Hepatoprotective and antioxidant activity of methanolic extract of flowers of Nerium oleander against CCl4-induced liver injury in rats. Asian Pac. J. Trop. Med., 5: 677-685.
27.
Sivakumar, G., D.R. Vail, V. Nair, F. Medina‐Bolivar and J.O. Lay, 2009. Plant-based corosolic acid: Future anti‐diabetic drug? Biotechnol. J., 4: 1704-1711.
28.
Somasundaram, A., R. Karthikeyan, V. Velmurugan, B. Dhandapani and M. Raja, 2010. Evaluation of hepatoprotective activity of Kyllinga nemoralis (Hutch and Dalz) rhizomes. J. Ethnopharmacol., 127: 555-557.
29.
Stohs, S.J., H. Miller and G.R. Kaats, 2012. A review of the efficacy and safety of banaba (Lagerstroemia speciosa L.) and corosolic acid. Phytother. Res., 26: 317-324.
30.
Tandon, V.R., V. Khajuria, B. Kapoor, D. Kour and S. Gupta, 2008. Hepatoprotective activity of Vitex negundo leaf extract against anti-tubercular drugs induced hepatotoxicity. Fitoterapia, 79: 533-538.
31.
Vijaykumar, K., P.B. Murthy, S. Kannababu, B. Syamasundar and G.V. Subbaraju, 2006. Quantitative determination of corosolic acid in Lagerstroemia speciosa leaves, extracts and dosage forms. Int. J. Applied Sci. Eng., 4: 103-114.
32.
Xu, Y., R. Ge, J. Du, H. Xin and T. Yi et al., 2009. Corosolic acid induces apoptosis through mitochondrial pathway and caspases activation in human cervix adenocarcinoma HeLa cells. Cancer Lett., 284: 229-237.
33.
Yang, L., C.Z. Wang, J.Z. Ye and H.T. Li, 2011. Hepatoprotective effects of polyprenols from Ginkgo biloba L. leaves on CCl4-induced hepatotoxicity in rats. Fitoterapia, 82: 834-840.
34.
Zeashan, H., G. Amresh, S. Singh and C.V. Rao 2008. Hepatoprotective activity of Amaranthus spinosus in experimental animals. Food Chem. Toxicol., 46: 3417-3421.
35.
Zilversmit, B.B. and A.K. Davis, 1950. Microdetermination of plasma phospholipids by trichloroacetic acid precipitation. J. Lab. Clin. Med., 35: 155-160.