In vitro Characteristics of Rumen Fermentation of Fattening Rations with Different Protein-energy Levels Fed to Bali Cattle

Authors

  • Paulus Klau Tahuk Department of Animal Science, Faculty of Agriculture, Timor University, Kefamenanu, East Nusa Tenggara, Indonesia
  • Subur Priyono Sasmito Budhi Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia
  • Panjono Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia
  • Endang Baliarti Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia

DOI:

https://doi.org/10.3923/pjn.2016.897.904

Keywords:

Bali cattle, fattening, in vitro, protein-energy, rumen fermentation, smallholder beef cattle farming

Abstract

Objective: This study investigated in vitro characteristics of fermentation of fattening rations with different protein-energy levels fed to male Bali cattle. Methodology: Rations were composed of grass, Gliricidia sepium, corn meal and rice bran with different proportions of protein and energy. Ration T1 was standard protein-standard energy/SS [(12.06% Crude Protein (CP) and 62.66% Total Digestible Nutrients (TDN)], T2 was standard protein-high energy/SH (10.14% CP and 65. 66% TDN), T3 was high protein-standard energy/HS (14.79% CP and 63.66% TDN) and T4 was high protein-high energy/HH (13% CP and 67.48% TDN). Data were analyzed by one-way analysis of variance. Results: Although, pH level of rumen fluid was similar for all treatments (p>0.05), digestibility of dry matter and organic matter in rations T4 and T3 was higher (p<0.01) than that for T1 and T2. For N-NH3 (mg/100 mL), the yield of T4 and T3 was higher (p<0.01) than that for T1 and T2. Meanwhile, total VFA, acetic and propionic acids in T2, T3 and T4 were higher (p<0.01) than for T1 but butyric acid levels for T4 were higher (p<0.01) than that for T1, T2 and T3 and the acetic: propionic acid ratio of T4 was lower (p<0.01) than that for T1, T2 and T3. Microbial protein synthesis (mg mL–1) for the T1 ration was higher (p<0.01) than that of T2, T3 and T4. Conclusion: Varying the protein and energy levels of rations fed to male Bali cattle did not affect rumen pH but the digestibility of dry matter and organic matter was unclear. Moreover, N-NH3 utilization and VFA yield were not optimal for protein biosynthesis by microbes.

References

Madsen, J. and T. Hvelplund, 1994. Prediction of in situ protein degradability in the rumen. Results of a European ringtest. Livestock Prod. Sci., 39: 201-212.

Owens, F.N. and W.G. Bergen, 1983. Nitrogen metabolism of ruminant animals: Historical perspective, current understanding and future implications. J. Anim. Sci., 57: 498-518.

Mullik, M.L., 2006. [Supplementation strategies to improve efficiency of rumen microbial protein synthesis on cattles fed with tropical grass hay]. Jurnal Ilmu Ternak dan Veteriner, 11: 15-23, (In Indonesian).

Klusmeyer, T.H., R.D. McCarthy Jr., J.H. Clark and D.R. Nelson, 1990. Effects of source and amount of protein on ruminal fermentation and passage of nutrients to the small intestine of lactating cows. J. Dairy Sci., 73: 3526-3537.

Tahuk, P.K., A.A. Dethan and Stefanus Sio, 2013. Kinerja sapi bali jantan pada penggemukan menggunakan pakan tunggal hijauan serta kombinasi hijauan dan konsentrat dengan level protein pakan berbeda di pulau timor. Laporan Penelitian Hibah Bersaing Perguruan Tinggi, Direktorat Jenderal Pendidikan Tinggi.

Buxton, D.R. and M.R. Brasche, 1991. Digestibility of structural carbohydrates in cool-season grass and legume forages. Crop Sci., 31: 1338-1345.

Soeparno and H.L. Davies, 1987. Studies on the growth and carcass composition in Daldale wether lambs. 2. The effect ofdietary protein/energy ratio. Crop Pasture Sci., 38: 417-426.

Kearl, L.C., 1982. Nutrient requirements of ruminants in developing countries. Ph.D. Thesis, International Feedstuff Institute, Utah Agricultural Experiment Station, Utah State University, Logan, UT., USA.

Tilley, J.M.A. and R.A. Terry, 1963. A two-stage technique for the in vitro digestion of forage crops. Grass Forage Sci., 18: 104-111.

Filipek, J. and R. Dvorak, 2009. Determination of the volatile fatty acid content in the rumen liquid: Comparison of gas chromatography and capillary isotachophoresis. Acta Veterinaria Brno, 78: 627-633.

Chaney, A.L. and E.P. Marbach, 1962. Modified reagents for determination of urea and ammonia. Clin. Chem., 8: 130-132.

Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.

Steel, R.G.D. and J.H. Torrie, 1995. Prinsip dan Prosedur Statistika. PT. Gramedia, Jakarta.

Erdman, R.A., 1988. Dietary buffering requirements of the lactating dairy cow: A review. J. Dairy Sci., 71: 3246-3266.

McDonald, P., R.A. Edward, J.F.D. Greehalgn and C.A. Morgan, 2002. Animal Nutrition. 6th Edn., Ashford Color Press, Gasport, USA., Pages: 567.

Bach, A., S. Calsamiglia and M.D. Stern, 2005. Nitrogen metabolism in the rumen. J. Dairy Sci., 88: E9-E21.

Harris, L.E., L.C. Kearl and P.V. Fonnesbeck, 1972. Use of regression equations in predicting availability of energy and protein. J. Anim. Sci., 35: 658-680.

Hartadi, H., S. Reksohadiprodjo, S. Lebdosukojo, A.D. Tillman, L.C. Kearl and L.E. Harris, 1980. Tables of Feed Composition for Indonesia. IFI. Utah Agricultural Experiment Station, Utah State University, Logan Utah.

Nitis, I.M., 2007. Gamal di lahan kering. Cetakan Pertama. Penerbit Arti Foundation, Denpasar-Bali.

Devant, M., A. Ferret, S. Calsamiglia, R. Casals and J. Gasa, 2001. Effect of nitrogen source in high-concentrate, low-protein beef cattle diets on microbial fermentation studied in vivo and in vitro. J. Anim. Sci., 79: 1944-1953.

Satter, L.D. and L.L. Slyter, 1974. Effect of ammonia concentration on rumen microbial protein production in vitro. Br. J. Nutr., 32: 199-208.

Thomas, P.C. and J.A.F. Rook, 1981. Manipulation of Rumen Fermentation. In: Recent Developments in Ruminant Nutrition, Haresign, W. and D.J.A. Cole (Eds.). Chapter 9, Butterworth Inc., London, ISBN-13: 9780408108041, pp: 157-183.

Orskov, E.R., 1992. Protein Nutrition of Ruminants. 2nd Edn., Academic Press, London, Pages: 175.

Min, B.R., T.N. Barry, G.T. Attwood and W.C. McNabb, 2003. The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages: A review. Anim. Feed Sci. Technol., 106: 3-19.

Maynard, L.A., J.K. Loosli, H.S. Hintz and R.G. Warner, 1979. Animal Nutrition. 7th Edn., Tata McGraw-Hill Publishing Company, New Delhi, India, Pages: 602.

Pilajun, R. and M. Wanapat, 2014. Effect of roughage to concentrate ratio and plant oil supplementation on in vitro fermentation end-products. Pak. J. Nutr., 13: 492-499.

Cherney, D.J.R., J.H. Cherney and L.E. Chase, 2003. Influence of dietary nonfiber carbohydrate concentration and supplementation of sucrose on lactation performance of cows fed fescue silage. J. Dairy Sci., 86: 3983-3991.

Suwandyastuti, S.N.O., 2007. [The Rumen metabolism product in male sheep]. Anim. Prod., 9: 9-13, (In Indonesian).

Perry, T.W., A.E. Cullison and R.S. Lowrey, 2003. Feed and Feeding. 6th Edn., Prentice Hall, New Jersey, USA., ISBN-13: 978-0130970473, Pages: 675.

Suwandyastuti, S.N.O., 2013. [Rumen metabolism product on growing cattle ongole grade]. Agripet, 13: 31-35, (In Indonesian).

Stern, M.D. and W.H. Hoover, 1979. Methods for determining and factors affecting rumen microbial protein synthesis: A review. J. Dairy Sci., 49: 1590-1603.

Nocek, J.E. and J.B. Russell, 1988. Protein and energy as an integrated system. Relationship of ruminal protein and carbohydrate availability to microbial synthesis and milk production. J. Dairy Sci., 71: 2070-2107.

Stern, M.D., A. Bach and S. Calsamiglia, 2006. New concepts in protein nutrition of ruminants. Proceedings of the 21st Annual Southwest Nutrition and Management Conference, February 23-24, 2006, Tempe, AZ., pp: 48.

Suwandyastuti, S.N.O. and E.A. Rimbawanto, 2015. [Rumen metabolism product on lactating dairy cattle]. Agripet, 15: 1-6, (In Indonesian).

Huber, J.T. and L. Kung Jr., 1981. Protein and nonprotein nitrogen utilization in dairy cattle. J. Dairy Sci., 64: 1170-1195.

Polan, C.E., 1988. Update: Dietary protein and microbial protein contribution. J. Nutr., 118: 242-248.

Huber, J.T. and R. Herrera-Saldana, 1994. Synchrony of Protein and Energy Supply to Enhance Fermentation. In: Principles of Protein Nutrition of Ruminants, Asplund, J.M. (Ed.). Chapter 6, CRC Press, Boca Raton, FL., USA., ISBN-13: 9780849349102, pp: 113-126.

Downloads

Published

15.09.2016

Issue

Section

Research Article

How to Cite

Tahuk, P. K., Budhi, S. P. S., Panjono, & Baliarti, E. (2016). In vitro Characteristics of Rumen Fermentation of Fattening Rations with Different Protein-energy Levels Fed to Bali Cattle. Pakistan Journal of Nutrition, 15(10), 897–904. https://doi.org/10.3923/pjn.2016.897.904

Most read articles by the same author(s)