The Effects of Different Sizes of Nanometer Zinc Oxide on the Proliferation and Cell Integrity of Mice Duodenum-Epithelial Cells in Primary Culture

Abstract

The MTT activity and LDH release were investigated in the proliferation and cell integrity of mice duodenum-epithelial cells which exposed to 10-15 nm and 100 nm nanometer zinc oxide (nano-ZnO). The mice duodenum-epithelial cells supplied two different sizes of nano-ZnO where supplemental Zn concentrations were 0, 0.4, 0.8, 1.6, 3.2, 6.4, 12.8 μg ml^-1, respectively. The results showed that the MTT activity increased with the increased concentrations of 10-15 nm and 100 nm nano-ZnO, while the concentration 12.8 μg ml ^-1 of 10-15 nm nano-ZnO decreased the MTT activity. The LDH released increased significantly under the concentration 12.8 μg ml^-1 of 100 nm nano-ZnO and 6.4 μg ml ^-1, 12.8 μg ml^-1 of 10-15 nm nano-ZnO. In summary, the nano-ZnO promoted the proliferation of the cells and did not injury the cells at lower concentrations.