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Chemical Characteristics and Antioxidant Content Properties of Cold Pressed Seed Oil of Wild Milk Thistle Plant Grown in Jordan
Abstract
This study aimed to examine the chemical characteristics and antioxidant content properties of cold-pressed milk thistle seed oil. Acidity and peroxide value were determined by chemical standard titration method and oxidative stability index was determined by Rancimate. Using GLC, fatty acid composition, phytosterols and squalene were measured. While, alpha-tocopherol content was determined by HPLC. The total phenolic content, radical-scavenging capacity and total antioxidant capacity were determined spectrophotometrically. The chemical characteristics of cold-pressed oil were as follows: acidity (0.64%), peroxide value (0.34 meq O2/kg of oil), oxidative stability index (55.7 and 12.9 h at 80 and 100°C, respectively), fatty acid composition (19.5, 22.9 and 57.6% for SAT, MUFA and PUFA, respectively), phytosterol (2520 mg/kg), squalene (9.35 mg/kg), alpha-tocopherol content (237.4 mg/kg), total phenolic content (1.16 mg GAE/g oil), radical-scavenging capacity against stable DPPH radical (IC50 = 3.34 mg/mL) and total antioxidant capacity (2.29 mmol/L). These data suggest that cold-pressed milk thistle seed oil may serve as dietary source of high PUFA and MUFA, phytosterols, phenolic compounds and natural antioxidants and can be a remarkable candidate for use in healthy food preparations mixed with other vegetable oils or alone.
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Antioxidant capacity, milk thistle seeds, phenolic content, phytosterols, squaleneArticle Metrics
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Copyright (c) 2014 Asian Network for Scientific Information
This work is licensed under a Creative Commons Attribution 4.0 International License.
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Dabbour, I., Al-Ismail, K., Takruri, H., & Azzeh, F. (2014). Chemical Characteristics and Antioxidant Content Properties of Cold Pressed Seed Oil of Wild Milk Thistle Plant Grown in Jordan. Pakistan Journal of Nutrition, 13(2), 67–78. https://doi.org/10.3923/pjn.2014.67.78
References
1.
Fadavi, A., M. Barzegar and M.H. Azizi, 2006. Determination of fatty acids and total lipid content in oilseed of 25 pomegranates varieties grown in Iran. J. Food Compos. Anal., 19: 676-680.
2.
Abu Jadayil, S., S.K. Tukan and H.R. Takruri, 1999. Bioavailability of iron from four different local food plants in Jordan. Plant Foods Human Nutr., 54: 285-294.
3.
Abu-Rajouh, K.S. and H.R. Takruri, 2000. Studing the nutritional value of milk thistle seeds. Arab J. Food Nutr., 2: 31-47.
4.
Aguilera, C.M., M.D. Mesa, M.C. Ramirez-Tortosa, M.T. Nestares, E. Ros and A. Gil, 2004. Sunflower oil does not protect against LDL oxidation as virgin olive oil does in patients with peripheral vascular disease. Clin. Nutr., 23: 673-681.
5.
Al-Ismail, K., S.M. Herzallah and A.S. Rustom, 2007. Antioxidant activities of some edible wild Mediterranean plants. Ital. J. Food Sci., 19: 287-296.
6.
AOCS, 1997. Official Methods and Recommended Practices of the American Oil Chemists Society. 5th Edn., AOCS Press, Champaign, USA.
7.
Apak, R., K. Guclu, B. Demirata, M. Ozyurek and S.E. Celik et al., 2007. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules, 12: 1496-1547.
8.
Arena, E., S. Campisi, B. Fallico and E. Maccarone, 2007. Distribution of fatty acids and phytosterols as a criterion to discriminate geographic origin of pistachio seeds. Food Chem., 104: 403-408.
9.
Arranz, S., R. Cert, J. Perez-Jimenez, A. Cert and F. Saura-Calixto, 2008. Comparison between free radical scavenging capacity and oxidative stability of nut oils. Food Chem., 110: 985-990.
10.
Bail, S., G. Stuebiger, S. Krist, H. Unterweger and G. Buchbauer, 2008. Characterisation of various grape seed oils by volatile compounds, triacylglycerol composition, total phenols and antioxidant capacity. Food Chem., 108: 1122-1132.
11.
Barreto, J.F.A., S.N. Wallace, D.J. Carrier and E.C. Clausen, 2003. Extraction of nutraceuticals from milk thistle: I. Hot water extraction. Applied Biochem. Biotechnol., 105-108: 881-889.
12.
Bozan, B. and F. Temelli, 2008. Chemical composition and oxidative stability of flax, safflower and poppy seed and seed oils. Bioresour. Technol., 99: 6354-6359.
13.
Cabrini, L., V. Barzanti, M. Cipollone, D. Fiorentini and G. Grossi et al., 2001. Antioxidants and total peroxyl radical-trapping ability of olive and seed J. Agric. Food Chem., 49: 6026-6032.
14.
Deraz, S. and E. Bayram, 1995. Evaluation of chemical contents of medicinal plant (Silybum marianum) wild growing in Turkey. Ege-Ueniversitesi-Ziraat-Fakueltes Dergisi., 32: 79-85.
15.
Dessi, M.A., M. Deiana, B.W. Day, A. Rosa, S. Banni and F.P. Corongiu, 2002. Oxidative stability of polyunsaturated fatty acids: Effect of squalene. Eur. J. Lipid Sci. Technol., 104: 506-512.
16.
European Committee, 2003. On the characteristics of Olive oil and olive residue oil and on the relevant methods of analysis. CONSLEG: 199 IR258-01/11/2005, Office for Official Publications of the European Communities, USA.
17.
Fathi-Achachlouei, B. and S. Azadmard-Damirchi, 2009. Milk thistle seed oil constituents from different varieties grown in Iran. J. Am. Oil Chem. Soc., 86: 643-649.
18.
Floegel, A., D.O. Kim, S.J. Chung, S.I. Koo and O.K. Chun, 2011. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J. Food Comp. Anal., 24: 1043-1048.
19.
Garjani, A., F. Fathiazad, A. Zakheri, N.A. Akbari and Y. Azarmie et al., 2009. The effect of total extract of Securigera securidaca L. seeds on serum lipid profiles, antioxidant status and vascular function in hypercholesterolemic rats. J. Ethnopharmacol., 126: 525-532.
20.
Giacometti, J., 2001. Determination of aliphatic alcohols, squalene, alpha-tocopherol and sterols in olive oils: Direct method involving gas chromatography of the unsaponifiable fraction following silylation. Analyst, 126: 472-475.
21.
Gimeno, E., A.I. Castellote, R.M. Lamuela-Raventos, M.C. De La Torre and M.C. Lopez-Sabater, 2000. Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography. J. Chromatogr. A, 881: 251-254.
22.
Gorinstein, S., H. Leontowicz, M. Leontowicz, A. Lojek and M. Ciz et al., 2003. Seed oils improve lipid metabolism and increase antioxidant potential in rats fed diets containing cholesterol. Nutr. Res., 23: 317-330.
23.
Hinneburg, I., H.J.D. Dorman and R. Hiltunen, 2006. Antioxidant activities of extracts from selected culinary herbs and spices. Food Chem., 97: 122-129.
24.
Hauenschild, A., R.G. Bretzel, H. Schnell-Kretschmer, H.U. Kloer, P.D. Hardt and N. Ewald, 2010. Successful treatment of severe hypertriglyceridemia with a formula diet rich in omega-3 fatty acids and medium-chain triglycerides. Ann. Nutr. Metab., 56: 170-175.
25.
Jacobs, B.P., C. Dennehy, G. Ramirez, J. Sapp and V.A. Lawrence, 2002. Milk thistle for the treatment of liver disease: A systematic review and meta-analysis. Am. J. Med., 113: 506-515.
26.
CODEX-STAN 210, 2005. Codex standards for named vegetable oils. CODEX-STAN 210 Amended 2005, Joint FAO/WHO Food Standards Program.
27.
Lagarda, M.J., G. Garcia-Llatas and R. Farre, 2006. Analysis of phytosterols in foods. J. Pharmaceut. Biomed. Anal., 41: 1486-1496.
28.
Lutterodt, H., M. Luther, M. Slavin, J.J. Yin, J. Parry, J.M. Gao and L. Yu, 2010. Fatty acid profile, thymoquinone content, oxidative stability and antioxidant properties of cold-pressed black cumin seed oils. LWT-Food Sci. Technol., 43: 1409-1413.
29.
Maguire, L.S., S.M. O'Sullivan, K. Galvin, T.P. O'Connor and N.M. O'Brien, 2004. Fatty acid profile, tocopherol, squalene and phytosterol content of walnuts, almonds, peanuts, hazelnuts and the macadamia nut. Int. J. Food Sci. Nutr., 55: 171-178.
30.
Moore, J., Z. Hao, K. Zhou, M. Luther, J. Costa and L. Yu, 2005. Carotenoid, tocopherol, phenolic acid and antioxidant properties of Maryland-grown soft wheat. J. Agric. Food Chem., 53: 6649-6657.
31.
Niki, E., 2010. Assessment of antioxidant capacity in vitro and in vivo. Free Radical Biol. Med., 49: 503-515.
32.
Nyam, K.L., C.P. Tan, O.M. Lai, K. Long and Y.B. Che Mana, 2009. Physicochemical properties and bioactive compounds of selected seed oils. LWT-Food Sci. Technol., 42: 1396-1403.
33.
Nyiredy, S., Z. Szucs, S. Antus and Z. Samu, 2008. New components from Silybum marianum L. fruits: A theory comes true. Chromatographia, 68: 5-11.
34.
Horwitz, W. and AOACI, 2000. Official Methods of Analysis of AOAC International. 17th Edn., Association of Official Analytical Chemists, Rockville, Maryland, ISBN-13: 9780935584677, Pages: 2200.
35.
Parker, T.D., D.A. Adams, K. Zhou, A. Harris and L. Yu, 2003. Fatty acid composition and oxidative stability of cold-pressed edible seed oils. J. Food Sci., 68: 1240-1243.
36.
Parks, S.W. and P.B. Addis, 1986. Further investigation of oxidized cholesterol derivatives in heated fats. J. Food Sci., 51: 647-659.
37.
Parry, J.W., Z. Hao, M. Luther, L. Su, K. Zhou and L.L. Yu, 2006. Characterization of cold-pressed onion, parsley, cardamom, mullein, roasted pumpkin and milk thistle seed oils. J. Am. Oil Chem., 83: 847-854.
38.
Parry, J.W., L. Su, M. Luther, K. Zhou, M.P. Yurawecz, P. Whittaker and L. Yu, 2005. Fatty acid composition and Antioxidant properties of cold-pressed marionberry, boysenberry, red raspberry and blueberry seed oils. J. Agric. Food Chem., 53: 566-573.
39.
Roberfroid, M.B., 2000. Defining Functional Foods. In: Functional Foods: Concept to Product, Gibson, G. and C. Williams (Eds.). 1st Edn., Woodhead Publishing Ltd., Cambridge, UK., pp: 9-27.
40.
Lercker, G. and M.T. Rodriguez-Estrada, 2000. Chromatographic analysis of unsaponifiable compounds of olive oils and fat-containing foods. J. Chromatogr. A, 881: 105-129.
41.
Shaker, E., H. Mahmoud and S. Mnaa, 2010. Silymarin, the antioxidant component and Silybum marianum extracts prevent liver damage. Food Chem. Toxicol., 48: 803-806.
42.
Skottova, N., R. Vecera, K. Urbanek, P. Vana, D. Walterova and L. Cvak, 2003. Effects of polyphenolic fraction of silymarin on lipoprotein profile in rats fed cholesterol-rich diets. Pharmacol. Res., 47: 17-26.
43.
Sobolova, L., N. Skottova, R. Vecera and K. Urbanek, 2006. Effect of silymarin and its polyphenolic fraction on cholesterol absorption in rats. Pharmacol. Res., 53: 104-112.
44.
Tuberose, C.I.G., A. Kowalczyk, E. Sarritzu and P. Cebras, 2007. Determination of antioxidant compounds and antioxidant activity in commercial oilseeds for food use. Food Chem., 103: 1494-1501.
45.
Tukan, S.K., H.R. Takruri and D.M. Al-Eisawi, 1998. The use of wild edible plants in the Jordanian diet. Int. J. Food Sci. Nutr., 49: 225-235.
46.
Vujasinovic, V., S. Djilas, E. Dimic, R. Romanic and A. Takaci, 2010. Shelf life of cold-pressed pumpkin (Cucurbita pepo L.) seed oil obtained with a screw press. J. Am. Oil Chem. Soc., 87: 1497-1505.
47.
Wagner, K.H., A. Kamal-Eldin and I. Elmadfa, 2004. Gamma-tocopherol-an underestimated vitamin? Ann. Nutr. Metab., 48: 169-188.
