Effect of Lysozyme Isolated from Hen Egg White in Elongation the Shelf Life of Iraqi Soft Cheese Made from Buffalo Milk
DOI:
https://doi.org/10.3923/pjn.2014.635.641Keywords:
ADV, Lysozyme, NPN, soft cheese, storage, total countAbstract
The present study examined the effect of partially purified hen egg white Lysozyme as a natural antimicrobial to prolong the shelf life of soft cheese. The mentioned enzyme with enzymatic activity equal to 39 unit/mL was added in two concentration 250 and 300 mg/kg curd of soft cheese made from buffalo milk treatments T1 and T2, respectively. Treatments cheese and control cheese (C) were storage for 15 days at 6±1°C. The results revealed that there were a significant differences in chemical composition between control and treatments of Iraqi Soft Cheese (ISC) at zero time, also the enzyme contributed clearly to reduce the development of total count of bacteria also the count of psychrophilic bacteria and yeast and mold in treatments cheese during storage, treatment T2 gave better result, in prolonged the shelf life of ISC as a regard to bacterial count and also to the results of the chemical analysis for protein degradation expressed as the percentage of non-protein nitrogen (NPN) from the total nitrogen (TN) (% NPN/TN), as well as fat degradation expressed as acid degree value (ADV) during storage and retained good quality all the periods of storage (15 days) according to the broader for BDI method compared with control cheese (C), which became outside the broader of acceptance after 3 days of storage. These results reflected on sensory evaluation result for T1 and T2 treatments which get the acceptance and the highest score along the period of storage.
References
Abd El-Salam, M.H. and S. El-Shibiny, 2011. A comprehensive review on the composition and properties of buffalo milk. Dairy Sci. Technol., 91: 663-699.
Abd El-Salam, M.H. and S. El-Shibiny, 2013. Bioactive peptides of buffalo, camel, goat, sheep, mare and yak milks and milk products. Food Rev. Int., 29: 1-23.
Al-Dhhan, 1983. Cheese Industry and its Type in the World. Dar al-Hikma Press, Mosul, Iraq.
AOAC., 2004. Association of Official Methods of Analytical Chem. 17th Edn., AOAC, Gaithersburg, Maryland.
Araujo, K.B.S., A.H.N. Rangel, F.C.E. Fonseca, E.M. Aguiar, A.A. Simplicio and L.P. Novaes, D.M. Lima Jr., 2012. Influence of the year and calving season on production, composition and mozzarella cheese yield of water buffalo in the state of Rio Grande Do Norte, Brazil. Ital. J. Anim. Sci., 11: 87-91.
Sameen, A., F.M. Anjum, N. Huma and H. Nawaz, 2008. Quality evaluation of mozzarella cheese from different milk sources. Pak. J. Nutr., 7: 753-756.
Benkerroum, N., 2008. Antimicrobial activity of lysozyme with special relevance to milk. Afr. J. Biotechnol., 7: 4856-4867.
Branen, J.K. and P.M. Davidson, 2004. Enhancement of nisin, lysozyme and monolaurin antimicrobial activities by ethylenediaminetetraacetic acid and lactoferrin. Int. J. Food Microbiol., 90: 63-74.
Rubio, C.A., 2014. The natural antimicrobial enzyme lysozyme is up-regulated in gastrointestinal inflammatory conditions. Pathogens, 3: 73-92.
Chang, H.M., C.C. Yang and Y.C. Chang, 2000. Rapid separation of lysozyme from chicken egg white by reductants and thermal treatment. J. Agric. Food Chem., 48: 161-164.
Cogan, T.M., 1980. Heat resistant lipases and proteinases and the quality of dairy product. Int. Dairy Fed. Doc., 118: 19-26.
Cunningham, F.E. and H. Linewever, 1965. Stabilization of egg-white proteins to pasteurizing temperature above 60°C. Food Technol., 19: 136-141.
Davidson, P.M., 2001. Chemical Preservatives and Natural Antimicrobial Compounds. In: Food Microbiology, Doyle, M.P. (Ed.). ASM Press, Washington D.C., pp: 601-602.
Deeth, H.C. and C.H. Fitz-Gerald, 2006. Lipolytic Enzymes and Hydrolytic Rancidity. In: Advanced Dairy Chemistry Volume 2 Lipids, Fox, P.F. and P.L.H. McSweeney (Eds.). Springer, USA., ISBN-13: 9780387263649, pp: 481-556.
De Roos, A.L., P. Walstra and T.J. Geurts, 1998. The association of lysozyme with casein. Int. Dairy J., 8: 319-324.
Doosh, K.S. and S.S. Lafta, 2012. Isolation and purification of lysozyme enzyme from mastitis milk and studying its inhibition activity against some microorganism. J. Food Ind. Nutr. Sci., 2: 153-159.
Doosh, K.S., S.S. Lafta and L.H. Alzaidy, 2012. Study the effect of Lysozyme enzyme isolated from healthy and mastitis cow's milk in elongation the shelif life of Iraqi soft cheese. J. Food Ind. Nutr. Sci., 2: 301-312.
El-Soda, M., N. Ezzat, S. Hassanein, F. El-Abassy and A. Wahbe, 1990. Acceleration of Ras cheese ripening. 1-Addition of commerical enzymes preparation. Egypt. J. Dairy Sci., 18: 171-182.
Grill, A.O. and R.A. Holley, 2003. Interactive inhibition of meat spoilage and pathogenic bacteria by lysozyme, Nisin and EDTA in the presence of nitrite and sodium chloride at 24°C. Int. J. Food Microbiol., 80: 251-259.
Harper, W.J. and H.E. Randolph, 1969. Microbiological quality and keeping properties. Milk Dairy Dairy Sci., 28: 2894-2894.
ISO 4833, 2003. Microbiology of food and animal feeding Stuffs-horizontal method for the enumeration of the microorganisms: Colony count technique at 30°C. International Standards Organization, Geneva, Switzerland.
ISO, 6611, 2004. Milk and milk product: Enumeration of colony-forming units of yeasts and moulds-Colony count technique at 25°C. http://www.iso.org/iso/home/store/catalogue_tc/catalogue_detail.htm?csnumber=40473.
Li-Chan, E., S. Nakai, J. Sim, D.B. Bragg and K.V. Lo, 1986. Lysozyme separation from egg white by cation exchange column chromatography. J. Food Sci., 51: 1032-1042.
Ling, E.R., 2008. A Text Book of Dairy Chemistry. Vol. 2, Chapman and Hall Ltd., London, UK.
Medhammar, E., R. Wijesinha-Bettoni, B. Stadlmayr, E. Nilsson, U.R. Charrondiere and B. Burlingame, 2012. Composition of milk from minor dairy animals and buffalo breeds: A biodiversity perspective. J. Sci. Food Agric., 92: 445-474.
Menard, O., S. Ahmad, F. Rousseau, V. Briard-Bion, F. Gaucheron and C. Lopez, 2010. Buffalo vs. cow milk fat globules: Size distribution, zeta-potential, compositions in total fatty acids and in polar lipids from the milk fat globule membrane. Food Chem., 120: 544-551.
Min, S., L.J. Harris and J.M. Krochta, 2005. Antimicrobial effects of lactoferrin, lysozyme and the lactoperoxidase system and edible whey protein films incorporating the lactoperoxidase system against Salmonella enteric and Escherichia coli O157: H7. J. Food Sci., 70: m332-m338.
Tripathy, N., R. Ahmad, S.H. Bang, J. Minbc and Y.B. Hahn, 2014. Tailored lysozyme-ZnO nanoparticle conjugates as anoantibiotics. Chem. Commun., 50: 9298-9301.
Park, S.I., M.A. Daeschel and Y. Zhao, 2006. Functional properties of antimicrobial lysozyme-chitosan composite films. J. Food Sci., 69: 215-221.
Sahoo, N.R., P. Kumar, B. Bhusan, T.K. Bhattacharya, S. Dayal and M. Sahoo, 2012. Lysozyme in livestock: A guide to selection for disease resistance: A review. J. Anim. Sci. Adv., 2: 347-360.
SAS., 2004. SAS/STAT users guide for personal computers. Release 7.0, SAS Institute Inc., Cary, NC., USA.
Simoes, M.G., R.E. Portal, J.G. Rabelo and C.L.L.F. Ferreira, 2014. Seasonal variations affect the physicochemical composition of bufallo milk and artisanal cheeses produced in Marajo Island (Pa, Brazil). Adv. J. Food Sci. Technol., 6: 81-91.
Suppakul, P., J. Miltz, K. Sonneveld and S.W. Bigger, 2003. Active packaging technologies with an emphasis on antimicrobial packaging and its applications. J. Food Sci., 68: 408-420.
Visser, S., 1993. Proteolytic enzymes and their relation to cheese ripening and flavor: An overview. J. Dairy Sci., 76: 329-350.
Calandrelli, M., 2014. Manual on the production of traditional Buffalo Mozzarella cheese. Food and Agriculture Organization of the United Nations. http://www.fao.org/ag/againfo/themes/documents/milk/mozzarella.pdf.
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