Isolation, Purification and Characterization of Lactoferrin from Goat Colostrum Whey
DOI:
https://doi.org/10.3923/pjn.2015.517.523Keywords:
Characterization, goat colostrum, isolation, lactoferrin, purificationAbstract
Lactoferrin is an important protein in many biological applications as a potential cancer treatment agent. In this study, lactoferrin was purified from goat colostrum by ion exchange chromatography through CM-Sephadex C-50 column and gel filtration chromatography through Sephadex G-200 column. The purification fold and yield were (20.83 time and 62.50%) respectively. The purity of lactoferrin to homogeneity was examined by polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) with single bond. Some biochemical characteristics of the purified Lactoferrin were determined, The molecular weight of LF were 80 and 79.50kDa as determined by gel filtration and SDS-PAGE respectively . The percentage of carbohydrate content in goat Lactoferrin was 10.4%, mean while the Iron percentage was 123 ppm and as a saturated percent 7.8%.
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