Research Article Open Access

Isolation, Purification and Characterization of Lactoferrin from Goat Colostrum Whey

Authors

CORRESPONDING AUTHOR
Zainab H. Abbas
College of Agriculture, Karbala University, Karbala, Iraq
|
Kifah S. Doosh
College of Agriculture, Baghdad University, Baghdad, Iraq
|
Nahi Y. Yaseen
Iraqi Center for Cancer and Medical Genetic Research, Al-Mustansiriyah University, Iraq
Published on: July 15, 2015
DOI 10.3923/pjn.2015.517.523
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Abstract

Lactoferrin is an important protein in many biological applications as a potential cancer treatment agent. In this study, lactoferrin was purified from goat colostrum by ion exchange chromatography through CM-Sephadex C-50 column and gel filtration chromatography through Sephadex G-200 column. The purification fold and yield were (20.83 time and 62.50%) respectively. The purity of lactoferrin to homogeneity was examined by polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) with single bond. Some biochemical characteristics of the purified Lactoferrin were determined, The molecular weight of LF were 80 and 79.50kDa as determined by gel filtration and SDS-PAGE respectively . The percentage of carbohydrate content in goat Lactoferrin was 10.4%, mean while the Iron percentage was 123 ppm and as a saturated percent 7.8%.

Keywords:

Characterization, goat colostrum, isolation, lactoferrin, purification

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Abbas, Z. H., Doosh, K. S., & Yaseen, N. Y. (2015). Isolation, Purification and Characterization of Lactoferrin from Goat Colostrum Whey. Pakistan Journal of Nutrition, 14(8), 517–523. https://doi.org/10.3923/pjn.2015.517.523
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