Research Article Open Access

Antioxidant and Antimicrobial Activities of Gambir (Uncaria gambir Roxb) Extracts and Their Application in Rendang

Authors

CORRESPONDING AUTHOR
Sri Melia
Faculty of Animal Science, Andalas University, West Sumatera, Indonesia
|
Deni Novia
Faculty of Animal Science, Andalas University, West Sumatera, Indonesia
|
Indri Juliyarsi
Faculty of Animal Science, Andalas University, West Sumatera, Indonesia
Published on: November 15, 2015
DOI 10.3923/pjn.2015.938.941
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Abstract

"Gambir" (Uncaria gambir Roxb) used in this study was taken from 50 Kota Distric, West Sumatera, Indonesia, which was extracted by ethyl acetate. The antioxidant and antimicrobial activities of gambir extract were investigated. The antioxidant activities (IC50) of gambir extracts which were evaluated by DPPH (1,1-diphenyl-2-picrylhydrazil) method, were 25.55 μg/ml and ascorbic acid was used as standard. Disc Diffusion Method was employed to evaluate the antimicrobial activity against some Gram-Positive bacteria (Staphylococcus aureus) and Gram-Negative (E. coli and Salmonella sp). Rendang was made with gambir extracts with level: 0, 0.25, 0.5, 0.75 and 1%. The data obtained were processed statistically, if the treatment shows significant results (p<0.05) then further tested by Duncan's Multiple Range Test (DMRT) with SPSS methods. The organoleptic test showed that addition of gambir extracts in Rendang was not significant (p>0.05) and could extend the shelf-life of Rendang.

Keywords:

Antimicrobial, antioxidant, gambir, Rendang

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Copyright (c) 2015 Asian Network for Scientific Information

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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How to Cite

Melia, S., Novia, D., & Juliyarsi, I. (2015). Antioxidant and Antimicrobial Activities of Gambir (Uncaria gambir Roxb) Extracts and Their Application in Rendang. Pakistan Journal of Nutrition, 14(12), 938–941. https://doi.org/10.3923/pjn.2015.938.941
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References

1.
Amir, M., M. Mujeeb, A. Khan, K. Ashraf, D. Sharma and M. Aqil, 2012. Phytochemical analysis and in vitro antioxidant activity of Uncaria gambir. Int. J. Green Pharmacy, 6: 67-72.
2.
Apea-Bah, F.B., M. Hanafi, R.T. Dewi, S. Fajriah and A. Darmawan et al., 2009. Assessment of the DPPH and α-glucosidase inhibitory potential of gambier and qualitative identification of major bioactive compound. J. Med. Plants Res., 3: 736-757.
3.
Choe, E. and D.B. Min, 2009. Mechanisms of antioxidants in the oxidation of foods. Comprehensive Rev. Food Sci. Food Saf., 8: 345-358.
4.
Craft, B.D., A.L. Kerrihard, R. Amarowicz and R.B. Pegg, 2012. Phenol-based antioxidants and the in vitro methods used for their assessment. Cmprehensive Rev. Food Sci. Food Saf., 11: 148-173.
5.
Dehpour, A.A., M.A. Ebrahimzadeh, N.S. Fazel and N.S. Mohammad, 2009. Antioxidant activity of the methanol extract of Ferula assafoetida and its essential oil composition. Grasas Aceites, 60: 405-412.
6.
Raut, A., Rahmawaty and Z.S. Amcilia, 2015. The condition of uncaria Gambir roxb. as one of important medicinal plants in North Sumatra Indonesia. Procedia Chem., 14: 3-10.
7.
Weecharangsan, W., P. Opanasopit, M. Sukma, T. Ngawhirunpat, U. Sotanaphun and P. Siripong, 2006. Antioxidative and neuroprotective activities of extracts from the fruit hull of mangosteen (Garcinia mangostana Linn.). Med. Princip. Pract., 15: 281-287.
8.
Isnawati, A., M. Raini, O.D. Sampurno, D. Mutiatikum, L. Widowati and R. Gitawati, 2012. [Characterization of 3 types gambir extract (Uncaria gambir Roxb) from Sumatera Barat]. Buletin Penelitian Kesehatan, 40: 201-208, (In Indonesian).