Research Article Open Access

Comparison Study on the Extraction of Gelatin from Nila Fish (Oreochromis nilotichus) Skin Using Acetic Acid and Citric Acid

Authors

CORRESPONDING AUTHOR
Suryanti
Research Center and Development of Marine and Fisheries Postharvest and Biotechnology, Ministry of Marine and Fisheries, Jl. KS. Tubun, Petamburan VI, Jakarta 12060, Indonesia
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Retno Indrati
Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jl. Sosio Yustisia, Bulaksumur, Yogyakarta 55281, Indonesia
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Hari Eko Irianto
Research Center and Development of Marine and Fisheries Postharvest and Biotechnology, Ministry of Marine and Fisheries, Jl. KS. Tubun, Petamburan VI, Jakarta 12060, Indonesia
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Djagal Wiseso Marseno
Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jl. Sosio Yustisia, Bulaksumur, Yogyakarta 55281, Indonesia
Published on: July 15, 2016
DOI 10.3923/pjn.2016.777.783
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Abstract

Methods of gelatin extraction from nila fish (Oreochromis nilotichus) skin were studied using acid treatment (acetic acid or citric acid) at various concentrations (0, 0.05, 0.10, 0.15, 0.20 and 0.25 M) and soaking times (0, 1, 2, 3, 4 and 5 h). Gelatin extracted from nila skin treated with 0.10 M acetic acid for 2 h produced the highest yield (25.33%) with a gel strength of 346.16 g bloom and a molecule size distribution of 38-241 kDa. Gelatin extracted from the skin treated with 0.05 M citric acid for 1 h resulted in a yield of 21.09% with a gel strength of 134.52 g bloom and a molecule size distribution of 23-145 kDa. Gelatin extracted by both acids had similar infra-red absorbance spectra, ranging from 1640 to 1239 cm-1. However, gelatin from nila skin treated with 0.10 M acetic acid for 2 h produced gelatin with a more homogenous molecular microstructure than nila skin treated with 0.05 M citric acid for 1 h.

Keywords:

Acid, fish, gelatin

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How to Cite

Suryanti, Indrati, R., Irianto, H. E., & Marseno, D. W. (2016). Comparison Study on the Extraction of Gelatin from Nila Fish (Oreochromis nilotichus) Skin Using Acetic Acid and Citric Acid. Pakistan Journal of Nutrition, 15(8), 777–783. https://doi.org/10.3923/pjn.2016.777.783
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References

1.
Ahmad, M. and S. Benjakul, 2011. Characteristics of gelatin from the skin of unicorn leatherjacket (Aluterus monoceros) as influenced by acid pretreatment and extraction time. Food Hydrocolloids, 25: 381-388.
2.
Amiza, M.A. and D.S. Aishah, 2011. Effect of drying and freezing of Cobia (Rachycentron canadum) skin on its gelatin properties. Int. Food Res. J., 18: 159-166.
3.
Anonymous, 2012. Gelatin Handbook. Gelatin Manufacture Institute of America, Amerika.
4.
AOAC., 1995. Official Methods of Analysis. 15th Edn., Association of Official Analytical Chemists, Washington, DC., USA.
5.
Ayudiarti, D.L., Tazwir, Suryanti and R. Peranginangin, 2007. Effect of fish gelatin concentration as binder on the quality and acceptability of syrup. J. Fish. Sci., 9: 134-141.
6.
Cebi, N., M.Z. Durak, O.S. Toker, O. Sagdic and M. Arici, 2016. An evaluation of Fourier transforms infrared spectroscopy method for the classification and discrimination of bovine, porcine and fish gelatins. Food Chem., 190: 1109-1115.
7.
Hashim, D.M., Y.B.C. Man, R. Norakasha, M. Shuhaimi, Y. Salmah and Z.A. Syahariza, 2010. Potential use of Fourier transform infrared spectroscopy for differentiation of bovine and porcine gelatins. Food Chem., 118: 856-860.
8.
Jackson, M. and H.H. Mantsch, 1995. The use and misuse of FTIR spectroscopy in the determination of protein structure. Crit. Rev. Biochem. Mol. Biol., 30: 95-120.
9.
Jamilah, B., K.W. Tan, M.R. Umi Hartina and A. Azizah, 2011. Gelatins from three cultured freshwater fish skins obtained by liming process. Food Hydrocolloids, 25: 1256-1260.
10.
Kent, M.J., N.D. Light and A.J. Bailey, 1985. Evidence for glucose-mediated covalent cross-linking of collagen after glycosylation in vitro. Biochem. J., 225: 745-752.
11.
Muyonga, J.H., C.G.B. Cole and K.G. Duodu, 2004. Extraction and physico-chemical characterisation of Nile perch (Lates niloticus) skin and bone gelatin. Food Hydrocolloids, 18: 581-592.
12.
Muyonga, J.H., C.G.B. Cole and K.G. Duodu, 2004. Fourier Transform Infrared (FTIR) spectroscopic study of acid soluble collagen and gelatin from skins and bones of young and adult Nile perch (Lates niloticus). Food Chem., 86: 325-332.
13.
Niu, L., X. Zhou, C. Yuan, Y. Bai, K. Lai, F. Yang and Y. Huang, 2013. Characterization of tilapia (Oreochromis niloticus) skin gelatin extracted with alkaline and different acid pretreatments. Food Hydrocolloids, 33: 336-341.
14.
Peranginangin, R., Mulyasari, A. Sari and Tazwir, 2005. Characteristic of quality Gelatin from Patin fish (Pangsius sp) bone by acid extracted. J. Post-Harvest Biotechnol. Mar. Fish., 4: 15-24.
15.
Peranginangin, R., Tazwir, Suryanti and D.A. Lestari, 2006. Optimization research of waste utilizationof fish bone and skin. Technical reports. Research Center for Marine and Fisheries Product Processing and Socio Economic, Jakarta.
16.
Pranoto, Y., D.W. Marseno and H. Rahmawati, 2011. Characteristics of gelatins extracted from fresh and sun-dried seawater fish skins in Indonesia. Int. Food Res. J., 18: 1335-1341.
17.
Schrieber, R. and H. Garies, 2007. Gelatin Handbook. WILEY-VCH Verlag GmbH and Co., USA.
18.
See, S.F., P.K. Hong, K.L. Ng, W.M. Wan Aida and A.S. Babji, 2010. Physicochemical properties of gelatins extracted from skins of different freshwater fish species. Int. Food Res. J., 17: 809-816.
19.
Setiyowati, E., 2014. Characterization of gelatinof nila (Oreochromis niloticus) skin and the application injellysweets. Master's Thesis, Gajah Mada University, Yogyakarta.
20.
Sinthusamran, S., S. Benjakul and H. Kishimura, 2014. Characteristics and gel properties of gelatin from skin of seabass (Lates calcarifer) as influenced by extraction conditions. Food Chem., 152: 276-284.
21.
SNI., 1995. Quality and Test methods of Gelatin. National Standardization Agency, Jakarta, Indonesia.
22.
Suryanti, R. Peranginangin and S. Hadi, 2006. Extraction of Gelatin from fish bone red kakap (Lutjanus sp.). J. Postharvest Biotechnol. Mar. Fish., 4: 27-34.
23.
Ward, A.G. and A. Coutrs, 1977. Technology of Gelatin. CRC Press, New York.