Effects of Cocoa Bean (Theobroma cacao L.) Fermentation on Phenolic Content, Antioxidant Activity and Functional Group of Cocoa Bean Shell
DOI:
https://doi.org/10.3923/pjn.2016.948.953Keywords:
Antioxidant activity, cocoa bean shell, DPPH, fermentation, folin-Ciocalteu, FTIR, functional group, total phenolic contentsAbstract
Background and Objective: Cocoa bean shell (CBS) has phenolic content and potential as cheaper source of antioxidants. The objective of this study was to evaluate the effects of cocoa bean fermentation on total phenolic content and antioxidant activity of cocoa bean shell. Materials and Methods: Cocoa beans were placed in fermentation box following spontaneous fermentation for 24, 48, 60, 72, 96 and 120 h. The CBS was separated from the cocoa beans and extracted with acetone-water (70:30 v/v). Folin-Ciocalteu method, 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and Fourier Transform Infrared Spectroscopy (FTIR) analysis were used to determine total phenolic contents, antioxidant activity and functional group of the samples, respectively. Results: For total phenolic contents, absorbance of various samples concentrations was plotted against gallic acid standard curve and the value indicated that CBS from partially fermented cocoa beans (24 h) contained the highest amount of phenolic content. Various concentrations of samples were also used for antioxidant activity measurement. Highest scavenging activity of 88.67±1.12% was obtained by 100 μg mL–1 CBS from partially fermented cocoa beans (24 h), while the lowest of 44.60±2.48% was obtained by CBS from 120 h fermented cocoa beans. A high correlation was observed between phenolic content and antioxidant activity. The FTIR analysis shows that CBS from 24 h fermented cocoa beans had more functional group compare to other samples. Conclusion: Fermentation of cocoa bean can affect total phenolic content and antioxidant activity of the CBS. Fermentation also causes functional group shift of CBS. The results of the study showed that CBS is a good source of antioxidants and phenolic compounds. Further study on the structural elucidation of the CBS individual phenolic compounds and evaluation of their mechanisms of action is recommended.
References
Kim, K.H., K.W. Lee, D.Y. Kim, H.H. Park, I.B. Kwon and H.J. Lee, 2004. Extraction and fractionation of glucosyltransferase inhibitors from cacao bean husk. Process Biochem., 39: 2043-2046.
MIRI., 2015. Industri kakao olahan serap investasi Rp 6 triliun. Ministry of Industry Republic of Indonesia (MIRI), September 21, 2015. http://www.beritasatu.com/ekonomi/253381-industri-kakao-olahan-serap-investasi-rp-6-triliun.html.
Adamson, G.E., S.A. Lazarus, A.E. Mitchell, R.L. Prior and G. Cao et al., 1999. HPLC method for the quantification of procyanidins in cocoa and chocolate samples and correlation to total antioxidant capacity. J. Agric. Food Chem., 47: 4184-4188.
Hammerstone, J.F., S.A. Lazarus, A.E. Mitchell, R. Rucker and H.H. Schmitz, 1999. Identification of procyanidins in cocoa (Theobroma cacao) and chocolate using high-performance liquid chromatography/mass spectrometry. J. Agric. Food Chem., 47: 490-496.
Bernaert, H., I. Blondeel, L. Allegaert and T. Lohmueller, 2012. Industrial Treatment of Cocoa in Chocolate Production: Health Implications. In: Chocolate and Health, Conti, A., R. Paoletti, A. Poli and F. Visioli (Eds.)., Springer, New York, USA, ISBN: 978-88-470-2037-5, pp: 17-31.
Kelm, M.A., J.C. Johnson, R.J. Robbins, J.F. Hammerstone and H.H. Schmitz, 2006. High-performance liquid chromatography separation and purification of cacao (Theobroma cacao L.) procyanidins according to degree of polymerization using a diol stationary phase. J. Agric. Food Chem., 54: 1571-1576.
Arlorio, M., J.D. Coisson, F. Travaglia, F. Varsaldi, G. Miglio, G. Lombardi and A. Martelli, 2005. Antioxidant and biological activity of phenolic pigments from Theobroma cacao hulls extracted with supercritical CO2. Food Res. Int., 38: 1009-1014.
Lecumberri, E., R. Mateos, M. Izquierdo-Pulido, P. Ruperez, L. Goya and L. Bravo, 2007. Dietary fibre composition, antioxidant capacity and physico-chemical properties of a fibre-rich product from cocoa (Theobroma cacao L.). Food Chem., 104: 948-954.
Bruna, C., I. Eichholz, S. Rohn, L.W. Kroh and S. Huyskens-Keil, 2009. Bioactive compounds and antioxidant activity of cocoa hulls (Theobroma cacao L.) from different origins. J. Applied Bot. Food Qual., 83: 9-13.
Martinez, R., P. Torres, M.A. Meneses, J.G. Figueroa, J.A. Perez-Alvarez and M. Viuda-Martos, 2012. Chemical, technological and in vitro antioxidant properties of cocoa (Theobroma cacao L.) co-products. Food Res. Int., 49: 39-45.
Nsor-Atindana, J., F. Zhong, K.J. Mothibe, M.L. Bangoura and C. Lagnika, 2012. Quantification of total polyphenolic content and antimicrobial activity of cocoa (Theobroma cacao L.) bean shells. Pak. J. Nutr., 11: 672-677.
Osawa, K., K. Miyazaki, S. Shimura, J. Okuda, M. Matsumoto and T. Ooshima, 2001. Identification of cariostatic substances in the cacao bean husk: Their anti-glucosyltransferase and antibacterial activities. J. Dental Res., 80: 2000-2004.
Ito, K., Y. Nakamura, T. Tokunaga, D. Iijima and K. Fukushima, 2003. Anti-cariogenic properties of a water-soluble extract from cacao. Biosci. Biotechnol. Biochem., 67: 2567-2573.
Zou, T., S.S. Percival, Q. Cheng, Z. Li, C.A. Rowe and L. Gu, 2012. Preparation, characterization and induction of cell apoptosis of cocoa procyanidins-gelatin-chitosan nanoparticles. Eur. J. Pharmaceut. Biopharmaceut., 82: 36-42.
Gu, L., S.E. House, X. Wu, B. Ou and R.L. Prior, 2006. Procyanidin and catechin contents and antioxidant capacity of cocoa and chocolate products. J. Agric. Food Chem., 54: 4057-4061.
Kim, H. and P.G. Keeney, 1984. (-)-Epicatechin content in fermented and unfermented cocoa beans. J. Food Sci., 49: 1090-1092.
Roelofsen, P.A., 1958. Fermentation, drying and storage of cacao beans. Adv. Food Res., 8: 225-296.
Bravo, L., 1998. Polyphenols: Chemistry, dietary sources, metabolism and nutritional significance. Nutr. Rev., 56: 317-333.
Sanbongi, C., N. Osakabe, M. Natsume, T. Takizawa, S. Gomi and T. Osawa, 1998. Antioxidative polyphenols isolated from Theobroma cacao. J. Agric. Food Chem., 46: 454-457.
Singleton, V.L., R. Orthofer and R.M. Lamuela-Raventós, 1999. Analysis of Total Phenols and other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. In: Methods in Enzymology, Packer, L. (Ed.), Academic Press, Cambridge, Massachusetts, ISBN: 9780121822002, pp: 152-178.
Burda, S. and W. Oleszek, 2001. Antioxidant and antiradical activities of flavonoids. J. Agric. Food Chem., 49: 2774-2779.
De Brito, E.S., N.H.P. Garcia, M.I. Gallao, A.L. Cortelazzo, P.S. Fevereiro and M.R. Braga, 2001. Structural and chemical changes in cocoa (Theobroma cacao L) during fermentation, drying and roasting. J. Sci. Food Agric., 81: 281-288.
Camu, N., T. de Winter, S.K. Addo, J.S. Takrama, H. Bernaert and L. de Vuyst, 2008. Fermentation of cocoa beans: Influence of microbial activities and polyphenol concentrations on the flavour of chocolate. J. Sci. Food Agric., 88: 2288-2297.
Mariani, L., 2011. Ekstraksi dan identifikasi senyawa polifenol dalam kulit biji kakao dan potensinya sebagai antioksidan. M.Sc. Thesis, Gadjah Mada University, Yogyakarta, Indonesia.
Afoakwa, E.O., J. Quao, F.S. Takrama, A.S. Budu and F.K. Saalia, 2012. Changes in total polyphenols, o-diphenols and anthocyanin concentrations during fermentation of pulp pre-conditioned cocoa (Theobroma cacao) beans. Int. Food Res. J., 19: 1071-1077.
Nazaruddin, R., L.K. Seng, O. Hassan and M. Said, 2006. Effect of pulp preconditioning on the content of polyphenols in cocoa beans (Theobroma cacao) during fermentation. Ind. Crops Prod., 24: 87-94.
Aikpokpodion, P.E. and L.N. Dongo, 2010. Effects of fermentation intensity on polyphenols and antioxidant capacity of cocoa beans. Int. J. Sustain. Crop Prod., 5: 66-70.
Lai, L.S., S.T. Chou and W.W. Chao, 2001. Studies on the antioxidative activities of Hsian-tsao (Mesona procumbens Hemsl) leaf gum. J. Agric. Food Chem., 49: 963-968.
Su, Y.L., J.Z. Xu, C.H. Ng, L.K. Leung, Y. Huang and Z.Y. Chen, 2004. Antioxidant activity of tea theaflavins and methylated catechins in canola oil. J. Am. Oil Chem. Soc., 81: 269-274.
Lin, C.C., C.W. Li, Y.T. Shih and L.T. Chuang, 2014. Antioxidant and anti-inflammatory properties of lower-polymerized polyphenols in oolong tea. Int. J. Food Propert., 17: 752-764.
Schinella, G., S. Mosca, S.E. Cienfuegos-Jovellanos, M.A. Pasamar, B. Muguerza, D. Ramon and J.L. Rios, 2010. Antioxidant properties of polyphenol-rich cocoa products industrially processed. Food Res. Int., 43: 1614-1623.
Shahidi, F., 1997. Natural Antioxidants: Chemistry, Health Effects and Applications. AOCS Press, Illinois, ISBN: 9780935315776, Pages: 414.
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