Electrophoretic Analysis of Seed Proteins from Different Varieties of Rice Cultivated in Sindh

Authors

  • Ibtessam Tahir Ansari Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan
  • Mumtaz Ali Sahito Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan
  • A.B. Ghanghro Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan
  • A.N. Memon Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan
  • Shaista Khan Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan

DOI:

https://doi.org/10.3923/pjn.2011.663.666

Keywords:

Genetic diversity, SDS-PAGE, seed storage proteins

Abstract

SDS-PAGE electrophoresis is the most effective as well as one of the simplest methods for separating proteins. The extraction and fractionation technique was applied to characterize rice seed proteins. Identification and screening of rice proteins carried out from 14 varieties of rice collected from Nuclear Institute of Agriculture (NIA) Tandojam. Rice seed proteins were extracted with phosphate buffer for total proteins. After quantification, proteins were separated by SDS-PAGE Electrophoresis. Analysis of rice seed proteins was performed on the results of SDS-PAGE using gel documentation system (Biorad) to find out the molecular weight of different varieties of rice followed by cluster analysis using SPSS to find out the diversity among different rice varieties.

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Published

15.06.2011

Issue

Vol. 10 No. 7 (2011)

Section

Research Article

License

Copyright (c) 2011 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Tahir Ansari, I., Sahito, M. A., Ghanghro, A., Memon, A., & Khan, S. (2011). Electrophoretic Analysis of Seed Proteins from Different Varieties of Rice Cultivated in Sindh. Pakistan Journal of Nutrition, 10(7), 663–666. https://doi.org/10.3923/pjn.2011.663.666