Changes in Sensory, Physicochemical and Microbiological Properties of Ronto During Fermentation
DOI:
https://doi.org/10.3923/pjn.2017.629.637Keywords:
Fermented shrimp, microbiology, physicochemical, ronto, sensoryAbstract
Background and Objective: Ronto is a traditional Indonesian fermented shrimp product; however, there are very few reports on how its properties change during fermentation. The aim of this study was to study the changes in sensory, physicochemical and microbiological properties of ronto during fermentation. Materials and Methods: Shrimp (Acetes japonicus) was mixed with salt and rice to give a mixture that was 11.5% salt and 20% rice. The mixture was put into plastic bottles and incubated at room temperature for 18 days. Samples were taken after every 2 days and sensory, physicochemical and microbiological properties were analyzed. Results: It was found that the fermented shrimp developed a sour, savory flavor and bright pink color after 8 days of fermentation, both of which became stronger after 12 days. After 14 days of fermentation, the texture of the shrimp gradually became that of a porridge-like paste. The acidity of the fermented shrimp increased during fermentation, after 12 days of fermentation, the pH had decreased from 8.3-4.8. The total volatile bases increased from 25-150 mg N/100 g over 18 days of fermentation. Lactic acid, proteolytic and amylolytic bacteria were involved in the fermentation process. The concentrations of these three bacteria increased during the early phases of fermentation and decreased in the later phases. Conclusion: Ronto fermentation ended after 14 days when the color of the fermented material became bright pink and when it had developed a strong, sour and savory flavor. That product had a lightness (53.11), a redness (11.72), a yellowness (15.68), a pH (4.8) and the texture of porridge-like paste and was relatively low in total volatile bases.
References
Khairina, R., Y. Fitrial, H. Satrio and N. Rahmi, 2016. Physical, chemical and microbiological properties of “Ronto” a traditional fermented shrimp from South Borneo, Indonesia. Aquat. Procedia, 7: 214-220.
Dyastuti, E.A., R. Nofiani and P. Ardiningsih, 2013. [The organoleptic test of cincalok with addition garlic (Allium sativum) and chili (Capsicum annuum L.) powder]. J. Kimia Khatulistiwa, 2: 70-73, (In Indonesian).
Sastra, W., 2008. Rusip fermentation. Skripsi, Fishery Faculty and Marine Science, Institut Pertanian Bogor, Indonesia, pp: 1-135.
Yuliana, N., 2007. [Profile of rusip fermentation used anchovies (Stolephorus sp.)]. Agritech, 27: 12-17, (In Indonesian).
Koesoemawardhani, D., S. Rizal and M. Tauhid, 2013. [Microbiological and chemical changes of rusip during fermentation]. Agritech, 33: 265-272, (In Indonesian).
Ibrahim, B., W. Zahiruddin and W. Sastra, 2009. Fermentation of rusip. Proceedings of the National Conference of Indonesian Fishery, December 3-4, 2009, High School of Fishing, Jakarta, pp: 314-320.
Huda, N., 2012. Malaysian Fermented Fish Products. In: Handbook of Animal-Based Fermented Food and Beverage Technology, Hui, Y.H. (Ed.). CRC Press/Taylor and Francis, USA., ISBN: 978-1-4398-5023-7, pp: 709-716.
Hajar, S. and T.H.T.A. Hamid, 2013. Isolation of lactic acid bacteria strain Staphylococcus piscifermentans from Malaysian traditional fermented shrimp cincaluk. Int. Food Res. J., 20: 125-129.
Steinkraust, K.H., 1992. Lactic Acid Fermentations. In: Application of Biotechnology in Traditional Fermented Foods, NRC (Eds.). Chapter 5, National Academy Press, Washington, DC., USA., pp: 43-51.
Rhee, S.J., J.E. Lee and C.H. Lee, 2011. Importance of lactic acid bacteria in Asian fermented foods. Proceeding of the 10th Symposium on Lactic Acid Bacterium, August 28-September 1, 2011, Egmond aan Zee, The Netherlands.
Adams, M., 2009. Fermented Fish. In: Microbiology Handbook: Fish and Seafood, Fernandes, R. (Ed.). Leatherhead Food International, Illinois, USA., pp: 123-140.
Faithong, N., S. Benjakul, S. Phatcharat and W. Binsan, 2010. Chemical composition and antioxidative activity of Thai traditional fermented shrimp and krill products. Food Chem., 119: 133-140.
Reerueangchai, P., Y. Suwannarat and J. Hinsui, 2014. Chemical and microbiological changes during shrimp seasoning fermentation using seafood processing waste. Proceedings of the 3rd International Conference on Nutrition and Food Sciences, June 18-20, 2014, Copenhagen, Demark, pp: 51-55.
Chaijan, M. and W. Panpipat, 2012. Darkening prevention of thai fermented shrimp paste by pre-soaking whole shrimp with pyrophosphate. Asian J. Food Ag-Ind., 5: 163-171.
AOAC., 1995. Official Methods of Analysis. 16th Edn., Association of Official Analytical Chemists, Washington, DC, USA.
Connell, J.J., 1990. Control of Fish Quality. 3rd Edn., Fishing News Books, Oxford, Pages: 245.
Sudarmadji, S., B. Haryono and Suhardi, 1984. The Analytical Procedure of Foods and Agriculture. 3rd Edn., Penerbit Liberty, Yogyakarta, Indonesia.
Wade, N.M., M. Anderson, M.J. Sellars, R.K. Tume, N.P. Preston and B.D. Glencross, 2012. Mechanisms of colour adaptation in the prawn Penaeus monodon. J. Exp. Biol., 215: 343-350.
Tume, R.K., A.L. Sikes, S. Tabrett and D.M. Smith, 2009. Effect of background colour on the distribution of astaxanthin in black tiger prawn (Penaeus monodon): Effective method for improvement of cooked colour. Aquaculture, 296: 129-135.
Jaswir, I., D. Noviendri, R.F. Hasrini and F. Octavianti, 2011. Carotenoids: Sources, medicinal properties and their application in food and nutraceutical industry. J. Med. Plants Res., 5: 7119-7131.
Pongsetkul, J., S. Benjakul, P. Sampavapol, K. Osako and N. Faithong, 2014. Chemical composition and physical properties of salted shrimp paste (Kapi) produced in Thailand. Int. Aquat. Res., 6: 155-166.
Hall, G.M., 2002. Lactic Acid Bacteria in Fish Preservation. In: Safety and Quality Issues in Fish Processing, Bremner, H.A. (Ed.). Chapter 17, Woodhead Publishing, Cambridge, UK., ISBN-13: 9780849315404, pp: 330-350.
Khairunnisak, M., A.H. Azizah, S. Jinap and A. Nurul Izzah, 2009. Monitoring of free glutamic acid in Malaysian processed foods, dishes and condiments. Food Addit. Contam.: Part A, 26: 419-426.
Jamias-Apilado, R.B. and R.C. Mabesa, 1991. Influence of rice and salt on the rate of rice-fish fermentation. Philipp. J. Biotechnol, 1: 160-160.
Shirai, K., I. Guerrero, S. Huerta, G. Saucedo, A. Castillo, Gonzalez and G.M. Hall, 2001. Effect of initial glucose concentration and inoculation level of lactic acid bacteria in shrimp waste ensilation. Enzyme Microbial Techol., 28: 446-452.
Sikorski, Z.E., 2001. Chemical and Functional Properties of Food Proteins. CRC Press, Boca Raton, FL., USA., ISBN-13: 9781566769600, Pages: 504.
Ozyurt, G., E. Kuley, S. Ozkutuk and F. Ozogul, 2009. Sensory, microbiological and chemical assessment of the freshness of red mullet (Mullus barbatus) and goldband goatfish (Upeneus moluccensis) during storage in ice. Food Chem., 114: 505-510.
Bechtel, P.J., A. Morey, A.C.M. Oliveira, T.H. Wu, S. Plante and C.K. Bower, 2010. Chemical and nutritional properties of Pacific Ocean perch (Sebastes alutus) whole fish and by-products. J. Food Process. Preserv., 34: 55-72.
Haaland, H. and L.R. Njaa, 1988. Ammonia (NH3) and Total Volatile Nitrogen (TVN) in preserved and unpreserved stored, whole fish. J. Sci. Food Agric., 44: 335-342.
Paludan-Muller, C., M. Madsen, P. Sophanodora, L. Gram and P.L. Moller, 2002. Fermentation and microflora of plaa-som, a Thai fermented fish product prepared with different salt concentrations. Int. J. Food Microbiol., 73: 61-70.
Rao, M.S. and W.F. Stevens, 2006. Fermentation of shrimp biowaste under different salt concentrations with amylolytic and non-amylolytic Lactobacillus strains for chitin production. Food Technol. Biotechnol., 44: 83-87.
Yanglang, C. and S. Maneerat, 2014. Screening of glutaminase-producing bacteria from Kung-Som. Proceedings of the 26th Annual Meeting of the Thai Soceity for Biotechnology and International Conference, November 26-29, 2014, Mae Fah Luang University, Chiang Rai, Thailand, pp: 312-318.
Wikandari, P.R., Suparmo, Y. Marsono and E.S. Rahayu, 2012. [Potency of lactic acid bacteria isolated from bekasam as angiotensin converting enzyme inhibitor producing bacteria in fermentation of “Bekasam-Like” product]. Agritech, 32: 258-264, (In Indonesian).
Dangkhaw, N., S. Maneerat and P. Sumpavapol, 2012. Characterization of lactic acid bacteria isolated from Kung-Som, a traditional fermented shrimp, in respect of their probiotic properties. Proceedings of the International Conference on Nutrition and Food Sciences, July 23-24, 2012, Singapore, pp: 121-125.
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