Antioxidant Activity and Total Phenol Content of Ethanol Extract Takokak Fruit (Solanum torvum)

Authors

  • Nur Rahman Department of Community Nutrition, Bogor Agriculture University, Darmaga Campus, Bogor-16680, Indonesia
  • Sri Anna Marliyati Department of Community Nutrition, Bogor Agriculture University, Darmaga Campus, Bogor-16680, Indonesia
  • Muhammad Rizal Martua Damanik Department of Community Nutrition, Bogor Agriculture University, Darmaga Campus, Bogor-16680, Indonesia
  • Faisal Anwar Department of Community Nutrition, Bogor Agriculture University, Darmaga Campus, Bogor-16680, Indonesia

DOI:

https://doi.org/10.3923/pjn.2013.973.977

Keywords:

Antioxidant activity, phenol content, Solanum torvum

Abstract

Takokak is a potential source of antioxidants for scavenger free radical. This study carried out to examines the affect of ethanol concentration to the yield extract, total phenol and antioxidants activity in variety maturity stage takokak (Solanum torvum). Research design was experiment laboratorium. The yield was calculated by comparing the weight of extract with baseline weight, phenol assay using the Folin-Ciocalteu reagent and antioxidants activity assay using DPPH method. The result showed that ethanol concentration ethanol 70 and 96% was significantly affected the yield extract (p = 0.000) and (p = 0.000). Concentration 70 and 96% did not significantly affect on level phenol (p = 0.056) and (p = 0.342). Concentration 70% was significantly affected antioxidant activity (p = 0.014), but concentration 96% was not significanlyt affected (p = 0.114) on the variety maturity stage of Solanum torvum. The best extracts was ethanol extract 70% old dried mature fruits.

References

Ahmad, I.M., M.Y. Abdalla, N.H. Mustafa, E.Y. Qnais and F.A. Abdulla, 2009. Datura aqueous leaf extract enhances cytotoxicity via metabolic oxidative stress on different human cancer cells. Jordan J. Biol. Sci., 2: 9-14.

Alakilli, S.Y.M., 2010. Gene expression and histopathology alterations during rat mammary carcinogenesis induced by 7,12-dimethylbenz [a] anthracene and the protective role of Neem (Azadirachta indica) leaf extract. J. Am. Sci., 9: 843-859.

Perez-Amador, M.C., V.M. Ocotero, J.M.G. Castaneda and A.R.G. Esquinca, 2007. Alkaloids in Solanum torvum Sw (Solanaceae). Int. J. Exp. Bot., 76: 39-45.

Archana, N., M. Ramasamy and C.D. Raj, 2012. Pharmacological screening of ethanolic extract of Syzygium cumini seed on isolated smooth muscle strip and heart. Int. J. Pharm. Pharm. Sci., 4: 108-110.

Arif, M. and S. Fareed, 2011. Pharmacognostical studies and evaluation of total phenolic and flavonoid contents of traditionally utilized of Solanum torvum swt. Indian J. Nat. Prod. Res., 2: 218-224.

Abaci, Z.T. and B.M. Asma, 2013. Examination of some physiological and biochemical changes based on ripening in fruits of different types of apricots. J. Plant Sci., 1: 6-10.

Boonyuen, C., S. Wangkarn, O. Suntornwat and R. Chaisuksant, 2009. Antioxidant capacity and phenolic content of Mimusops elengi fruit extract. Kasetsart J. (Nat. Sci.), 43: 21-27.

Kaur, C. and H.C. Kapoor, 2002. Anti-oxidant activity and total phenolic content of some Asian vegetables. Int. J. Food Sci. Technol., 37: 153-161.

Aboul-Enein, A.M., F.A. El-Ela, E.A. Shalaby and H.A. El-Shemy, 2012. Traditional medicinal plants research in Egypt: Studies of antioxidant and anticancer activities. J. Med. Plant Res., 6: 689-703.

Fox, A.J., D.D. Pozo-Insfran, J.H. Lee, S.H. Sargent and S.T. Talcott, 2005. Ripening-induced chemical and antioxidant changes in bell pepers as affected by harvest maturity and postharvest ethylene exposure. HortScience, 40: 732-736.

Kamble, S., M. Mohan and S. Kasture, 2009. Protective effect of Solanum torvum on doxorubicin-induced cardiactoxicity in rats. Pharmacologyonline, 2: 1192-1204.

Kusirisin, W., C. Jaikang, C. Chaiyasut and P. Narongchai, 2009. Effect of polyphenolic compounds from Solanum torvum on plasma lipid peroxidation, superoxide anion and cytochrome P450 2E1 in human liver microsomes. Med. Chem., 5: 583-588.

Loganayaki, N., P. Siddhuraju and S. Manian, 2010. Antioxidant activity of two traditional Indian vegetables: Solanum nigrum L. and Solanum torvum L. Food Sci. Biotechnol., 19: 121-127.

Nithiyanantham, S., S. Varadharajan and P. Siddhuraju, 2012. Differential effects of processing methods on total phenolic content, antioxidant and antimicrobial activities of three species of solanum. J. Food Drug Anal., 20: 844-854.

Michalak, A., 2006. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish J. Environ. Stud., 15: 523-530.

Satheesh, K., C.T. Sulaiman and I. Balachandran, 2013. Phytochemical studies on selected Gymnema species from kerala. Int. J. Pharmacognosy Phytochem. Res., 5: 143-146.

Scalbert, A., I.T. Johnson and M. Saltmarsh, 2005. Polyphenols: Antioxidants and beyond. Am. J. Clin. Nutr., 81: 215S-217S.

Waghulde, H., S. Kamble, P. Patankar, B. Jaiswal, S. Pattanayak, C. Bhagat and M. Mohan, 2011. Antioxidant activity, phenol and flavonoid contents of seeds of Punica granatum (Punicaceae) and Solanum torvum (Solanaceae). Pharmacologyonline, 1: 193-202.

Wetwitayaklung, P. and T. Phaechamud, 2011. Antioxidant activities and phenolic content of Solamun and Capsicum sp. Res. J. Pharm. Biol. Chem. Sci., 2: 146-154.

Yogananth, N., R. Bhakyaraj, A. Chanthuru, S. Parvathi and S. Palanivel, 2009. Comparative analysis of solasodine from in vitro and in vivo cultures of Solanum nigrum Linn. Kathmandu Univ. J. Sci. Eng. Technol., 5: 99-103.

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Published

15.10.2013

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Section

Research Article

How to Cite

Rahman, N., Marliyati, S. A., Damanik, M. R. M., & Anwar, F. (2013). Antioxidant Activity and Total Phenol Content of Ethanol Extract Takokak Fruit (Solanum torvum). Pakistan Journal of Nutrition, 12(11), 973–977. https://doi.org/10.3923/pjn.2013.973.977

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