Digestibility and Methane Emission of Ration Based on Oil Palm By Products Supplemented with Probiotics and Banana Stem: An In vitro Study
DOI:
https://doi.org/10.3923/pjn.2015.37.43Keywords:
Acetogen, banana, digestibility, methane, rumen, yeastAbstract
The objective of the present study was to investigate feed digestibility and methane emissions of ration based on oil palm by-products on addition of probiotics, namely Acetoanaerobium noterae and Saccharomyces cerevisiae and banana stem in vitro. The substrate, i.e., oil palm by-products consisted of oil palm midrib, oil palm leaf, oil palm kernel cake and oil palm sludge in the ratio of 30, 30, 30 and 10%, respectively. The following treatments were tested: control (R0), R0+S. cerevisiae (R1), R0+A. noterae (R2), R0+S. cerevisiae+A. noterae (R3), R0+ banana stem (R4), R0+banana stem+S. cerevisiae (R5), R0+banana stem+A. noterae (R6) and R0+banana stem+S. cerevisiae+A. noterae (R7). The treatments were incubated in vitro with buffered-rumen fluid in four replicates (represented by three incubation units per replicate), conducted for 48 h at 39oC. Gas production and methane emission were measured at regular time point intervals. After the incubation, digestibility, Volatile Fatty Acids (VFA), ammonia and microbial counts were determined. Results showed that the highest dry matter digestibility was shown by R5 and the best reduction of methane emission was shown by R2 at 12 h of incubation. In conclusion, supplementation of probiotics did not affect the digestibility of ration based on oil palm by-products but A. noterae addition was potential to mitigate ruminal methane emission.
References
AOAC., 1997. Official Methods of Analysis. Association of Official Analytical Chemists, Arlington, VA., USA.
Ando, S., R.I. Khan, J. Takahasi, Y. Gamo, R. Morikawa, Y. Nishiguchi and K. Hayasaka, 2004. Manipulation of rumen fermentation by yeast: The effects of dried beer yeast on the in vitro degradability of forages and methane production. Asian-Aust. J. Anim. Sci., 17: 68-72.
Campbell, J.R., M.D. Keneally and K.L. Campbell, 2003. Animal Sciences. 4th Edn., McGraw Hill Companies, New York, USA.
Chaucheyras, F., G. Fonty, G. Bertin and P. Gouet, 1995. In vitro H2 utilization by a ruminal acetogenic bacterium cultivated alone or in association with an archaea methanogen is stimulated by a probiotic strain of Saccharomyces cerevisiae. Applied Environ. Microbiol., 61: 3466-3467.
Conway, E.J., 1950. Microdiffusion Analysis and Volumetric Error. 3rd Edn., Crosby Lokswood and Sons, Ltd., London, Pages: 391.
Cottle, D.J., J.V. Nolan and S.G. Wiedeman, 2011. Ruminant enteric methane mitigation: A review. Anim. Prod. Sci., 51: 491-514.
Damron, W.S., 2006. Introduction to Animal Science. Prentice Hall, Ohio.
Dawson, K.A., K.E. Newman and J.A. Boling, 1990. Effect of microbial supplements containing yeast and Lactobacilli on roughage-fed ruminal microbial activities. J. Anim. Sci., 68: 3392-3398.
Fievez, V., O.J. Babayemi and D. Demeyer, 2005. Estimation of direct and indirect gas production in syringes: A tool to estimate short chain fatty acid production that requires minimal laboratory facilities. Anim. Feed Sci. Technol., 123-124: 197-210.
Fonty, G., K. Joblin, M. Chavarot, R. Roux, G. Naylor and F. Michallon, 2007. Establishment and development of ruminal hydrogenotrophs in metanogen-free lambs. Applied Environ. Microbiol., 73: 6391-6403.
Getachew, G., M. Blummel, H.P.S. Makkar and K. Becker, 1998. In vitro gas measuring techniques for assessment of nutritional quality of feeds: A review. Anim. Feed Sci. Technol., 72: 261-281.
Hart, K.J., D.R. Yanez-Ruiz, S.M. Duval, N.R. McEwan and C.J. Newbold, 2008. Plant extracts to manipulate rumen fermentation. Anim. Feed Sci. Technol., 147: 8-35.
Hiai, S., H. Oura and T. Nakajima, 1976. Color reaction of some sapogenins and saponins with vanillin and sulfuric acid. Planta Medica, 29: 116-122.
Jayanegara, A., M. Kreuzer, E. Wina and F. Leiber, 2011. Significance of phenolic compounds in tropical forages for the ruminal bypass of polyunsaturated fatty acids and the appearance of biohydrogenation intermediates as examined in vitro. Anim. Prod. Sci., 51: 1127-1136.
Jayanegara, A., S. Marquardt, E. Wina, M. Kreuzer and F. Leiber, 2013. In vitro indications for favourable non-additive effects on ruminal methane mitigation between high-phenolic and high-quality forages. Br. J. Nutr., 109: 615-622.
Jayanegara, A., E. Wina and J. Takahashi, 2014. Meta-analysis on methane mitigating properties of saponin-rich sources in the rumen: Influence of addition levels and plant sources. Asian-Australasian J. Anim. Sci., 27: 1426-1435.
Lopez, S., F.M. Mcintosh, R.J. Wallace and C.J. Newbold, 1999. Effect of adding acetogenic bacteria on methane production by mixed rumen microorganisms. Anim. Feed Sci. Technol., 78: 1-9.
Makkar, H.P.S., 2003. Quantification of Tannins in Tree and Shrub Foliage: A Laboratory Manual. Springer, Dordrecht, The Netherlands, ISBN: 9781402016325, Pages: 102.
Martin, C., J. Rouel, J.P. Jouany, M. Doreau and Y. Chilliard, 2008. Methane output and diet digestibility in response to feeding dairy cows crude linseed, extruded linseed or linseed oil. J. Anim. Sci., 86: 2642-2650.
McDonald, P., R.A. Edwards, J.F.D. Greenhalgh and C.A. Morgan, 2002. Animal Nutrition. 6th Edn., Prentice Hall, UK., ISBN: 9780582419063, Pages: 693.
McAllister, T.A. and C.J. Newbold, 2008. Redirecting rumen fermentation to reduce methanogenesis. Aust. J. Exp. Agric., 48: 7-13.
Min, B.R., T.N. Barry, G.T. Attwood and W.C. McNabb, 2003. The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages: A review. Anim. Feed Sci. Technol., 106: 3-19.
Morvan, B., F. Bonnemoy, G. Fonty and P. Gouet, 1996. Quantitative determination of H2-utilizing acetogenic and sulfate-reducing bacteria and methanogenic archaea from digestive tract of different mammals. Curr. Microbiol., 32: 129-133.
Oghimoto, K. and S. Imai, 1980. Atlas of Rumen Microbiology. Japan Scientific Societies Press, Tokio.
Reed, G. and T.W. Nagodawithana, 1991. Yeast Technology. 2nd Edn., Van Nostrand Reinhold, New York.
Smith, A.H., E. Zoetendal and R.I. Mackie, 2005. Bacterial mechanisms to overcome inhibitory effects of dietary tannins. Microb. Ecol., 50: 197-205.
Thalib, A., 2002. Effect of additional of microbial growth factors combined with and without microbe preparate on growth performance of Etawah-cross goat. JITV., 7: 220-226.
Thalib, A., 2004. In vitro study of effectiveness of saponin from Sapindus rarak fruit as methanogenesis inhibitor on ruminal digestion system. JITV., 9: 164-171.
Thalib, A., 2004. Isolation and identification of acetogenic bacteria obtained from deer rumen and their potential for methanogenesis inhibitor. JITV., 13: 197-206.
Theodorou, M.K. and A.E. Brooks, 1990. Evaluation of a new laboratory procedure for estimating the fermentation kinetics of tropical feeds: Annual report. AFRC Inst. Hurley, Meidenhead, UK.
Van Soest, P.J., 1982. Nutritional Ecology of Ruminant: Ruminant Metabolism, Nutritional Strategies, The Cellulolytic Fermentation and The Chemistry of Forages and Plant Fibers. O & B Books, New York, ISBN-13: 978-0960158607, Pages: 374.
van Soest, P.J., J.B. Robertson and B.A. Lewis, 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74: 3583-3597.
Wina, E., 2000. Utilization of yeast as an additive feed to improve the productivity of ruminant livestock. Wartazoa, 9: 50-56.
Wina, E., 2001. Banana plants as ruminant feed. Wartazoa, 11: 20-27.
Wina, E., 2012. The use of plant bioactive compounds to mitigate enteric methane in ruminants and its application in Indonesia. Wartazoa, 22: 24-34.
Yoon, I.K. and M.D. Stern, 1996. Effects of Saccharomyces cerevisiae and Aspergillus oryzae cultures on ruminal fermentation in dairy cows. J. Dairy Sci., 79: 411-417.
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