The Combined Effects of Fungi Phanerochaete chrysosporium and Neurospora crassa and Fermentation Time to Improve the Quality and Nutrient Content of Palm Oil Sludge
DOI:
https://doi.org/10.3923/pjn.2019.437.442Keywords:
Fermentation, Fermentation time, Neurospora crassa, nitrogen retention, nutrient content, palm oil sludge, Phanerochaete chrysosporiumAbstract
Background and Objective: Palm oil sludge, as a byproduct of the palm oil industry, is an agricultural waste product that can be used as an alternative feedstuff for poultry. Palm oil sludge also contains nutrients that can be used as feed ingredients for poultry. Palm oil sludge is limited in use in broiler rations because of its low quality and nutrient content. For this reason, it is necessary to process palm oil sludge by fermentation methods to improve the quality and the nutrient content. This study aimed to determine the combined effect of fungi (P. chrysosporium and N. crassa) and fermentation time to improve the quality of fermented palm oil sludge. Materials and Methods: The materials used in this study were palm oil sludge, the fungi Phanerochaete chrysosporium and Neurospora crassa and fermentation materials and tools. This experimental study used a completely randomized design (CRD) with a 3×3 factorial pattern with 3 replications. The treatments consisted of factor A (combination of Phanerochaete chrysosporium and Neurospora crassa), which consisted of A1 (3:1), A2 (3:2) and A3 (4:1) and factor B (fermentation time), which consisted of B1 (7 days), B2 (10 days) and B3 (13 days). Results: The results of the analysis of variance showed that there was a significant interaction (p<0.05) between factor A and factor B. Each factor A and B showed a significant effect (p<0.05). Conclusion: In this study, it was concluded that the combination of Phanerochaete chrysosporium and Neurospora crassa (4:1) and 13 days of fermentation time provided optimal results, with 26.20% crude protein, 14.49% crude fiber, 14.54% lignin, 58.20% nitrogen retention and 57.66% crude fiber digestibility.
References
Mirnawati , A. Djulardi and G. Ciptaan, 2018. Effect of fermented palm oil sludge with Neurospora crassa added to rations on broiler production performance. Pak. J. Nutr., 17: 487-491.
Directorate General of Plantations, 2015. Statistik perkebunan indonesia komoditas sawit tahun 2011-2014. Direktorat Jendral Perkebunan, Kementrian Pertanian, Jakarta.
Sianipar, J., L.P. Batubara, S.P. Ginting, K. Simanihuruk and A. Tarigan, 2003. Analisis potensi ekonomi limbah dan hasil ikutan perkebunan kelapa sawit sebagai pakan kambing potong. laporan hasil penelitian. Loka Penelitian Kambing Potong Sungai Putih, Sumatera Utara.
Devendra, C., 1977. Utilization of feedingstuffs from the oil palm. Proceedings of the Symposium on Feedingstuffs for Livestock in South East Asia, October 17-19, 1977, Malaysian Society of Animal Production, Serdang, Malaysia, pp: 116-131.
Mirnawati, A. Djulardi and G. Ciptaan, 2017. Role of humic acid in improving the nutrient content and quality of fermented palm oil sludge Pak. J. Nutr., 16: 538-543.
Sinurat, A.P., T. Purwadaria, P. Ketaren, D. Zainuddin and I.P. Kompiang, 2000. Utilization of palm oil sludge for poultry rations. J. Ilmu Ternak Vet., 5: 107-112.
Hutagalung, R.I., 1978. Non Tradisional Feeding Stuff for Livestock. In: Feeding Stuffs for Livestock in Southeast Asia, Hutagalung, R.I. (Ed.)., Malaysian Society of Animal Production, Serdang, Malaysia.
Lekito, M.N., 2002. Analyze the nutrient content of the palm oil sludge from the processing plantin Kecamatan Prafi, Kab. Manokwari, province. Papua. J. Peternakan Lingkungan, 8: 59-62.
Mahfudz, L.D., K. Hayashi, A. Ohtsuka and Y. Tomita, 1997. Effek shochu distillery by produk terhadap promosi pertumbuhan ayam broiler. Majalah Ilmiah Sain Teks, 4: 58-65.
Jay, M.J., 1978. Modern Food Microbiology. Van Nostrand Company, New York.
Zeng, G., M Yu, Y. Chen, D. Huang and J. Zhang et al., 2010. Effects of inoculation with Phanerochaete chrysosporium at various time points on enzyme activities during agricultural waste composting. Bioresour. Technol., 101: 222-227.
Noferdiman and A. Yani, 2013. Nutrition content of fermented palm oild sludge with Phanerochaete chrysosporium. Agripet, 13: 47-52.
Mirnawati, A. Djulardi and G. Ciptaan, 2015. Improving quality of palm kernel cake and palm oil sludge through biotechnology and application as low cholesterol feedstuff for poultry. Research Reports of Higher Education, Contract No. 030/SP2H/PL/DIT.LITABMAS/ii/2015, February 5, 2015.
Mirnawati, A. Djuliardi and G. Ciptaan, 2016. Improving quality of palm kernel cake and palm oil sludge through biotechnology and application as low cholesterol feedstuff for poultry. Contract No. 230/SP2H/LT/DRPM/III/2016, Maret 10.
Mandels, M. and D. Sternberg, 1976. Recent advances in cellulase technology. J. Ferment. Technol., 54: 267-286.
Steel, R.G.D. and J.H. Torrie, 1991. Prinsip and Prosedur Statistik: Suatu Pendekatan. PT Gramedia Pustaka Utama, Jakarta, Indonesia.
Iyayi, E.A., 2004. Changes in the cellulose, sugar and crude protein contents of agro-industrial by-products fermented with Aspergillus niger, Aspergillus flavus and Penicillium sp. Afr. J. Biotechnol., 3: 186-188.
Mirnawati, A. Djulardi and Y. Marlida, 2013. Improving the quality of palm kernel cake through fermentation by Eupenicillium javanicum as poultry ration. Pak. J. Nutr., 12: 1085-1088.
Iyayi, E.A., 2004. Changes in the cellulose, sugar and crude protein contents of agro-industrial by-products fermented with Aspergillus niger, Aspergillus flavus and Penicillium sp. Afr. J. Biotechnol., 3: 186-188.
Ofuya, C.O. and C.J. Nwajiuba, 1990. Fermentation of cassava peels for the production of cellulolytic enzymes. J. Applied Bacteriol., 68: 171-177.
Moore-Landecker, E., 1982. Fundamental of Fungi. Pretince Hall of Company, New York, USA.
Mirnawati, I.P. Kompiang and S.A. Latif, 2012. Effect of substrate composition and inoculum dosage to improve quality of palm kernel cake fermented by Aspergillus niger. Pak. J. Nutr., 11: 434-438.
Griffin, D.H., 1994. Fungal Physiology. 2nd Edn., John Wiley and Sons., New York, Pages: 458.
Rizal, Y., Nuraini, Mirnawati and M.E. Mahata, 2013. Comparisons of nutrient contents and nutritional values of palm kernel cake fermented by using different fungi. Pak. J. Nutr., 12: 943-948.
Fardiaz, S., 1988. Fisiologi Fermentasi. PAU Institut Pertanian, Bogor.
Valli, K., B.J. Brock, D.K. Joshi and M.H. Gold, 1992. Degradation of 2, 4-dinitrotoluene by the lignin-degrading fungus Phanerochaete chrysosporium. Applied Environ. Microbiol., 58: 221-228.
Maynard, L.A., J.K. Loosli, H.F. Hintz and R.G. Warner, 2005. Animal Nutrition. 7th Edn., McGraw-Hill Co., New York, USA.
Downloads
Published
Issue
Section
License
Copyright (c) 2019 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.