Effects of Extraction Media on Protein Isolates of Some Gourd Melon (Egusi) Seeds
DOI:
https://doi.org/10.3923/pjn.2015.983.987Keywords:
Extraction media, gourd melon (egusi) seeds, protein content, Sodium sulphiteAbstract
Protein isolates of Citrullus colocynthis, Citrullus vulgaris, Lageneria siceraria I (African Wine Kettle gourd), Lagenaria siceraria II (Basket Ball gourd) and Lageneria siceraria III (Bushel Giant Gourd) melon seeds were prepared using sodium sulphite extraction followed by precipitation at the isoelectric points of the seeds. Proximate composition of the isolates were determined using standard methods. Protein contents (%) of the protein isolates are relatively high, with values: 89.04, 89.44, 93.96, 90.42 and 90.63 (%) for Citrullus colocynthis, Citrullus vulgaris, Lageneria siceraria I, Lagenaria siceraria II and Lageneria siceraria III melon seeds protein isolates, respectively. The fat, fibre and carbohydrate contents of the protein isolates are however, relatively low.
References
Adebowale, Y.A., 2006. Some physicochemical properties of the flours and protein isolates from velvet beans (Mucuna species). Ph.D. Thesis, Department of Chemistry, Federal University of Technology, Akure, Ondo State.
Adebowale, Y.A., T. Henle and U. Schwarzenbolz, 2009. Acetylated and succinylated derivatives of African yam bean (Sphenostylis sternocarpa Hochst. Ex A. Rich.) Harms protein isolates. J. Mobile Commun., 3: 34-46.
AOAC., 1990. Official Methods of Analysis. 15th Edn., Association of Official Analytical Chemists, Washington, DC., USA., Pages: 684.
Aremu, M.O., I. Ogunlade and A. Olonisakin, 2007. Fatty acid and amino acid composition of protein concentrate from cashew nut (Anarcadium occidentale) grown in Nasarawa state, Nigeria. Pak. J. Nutr., 6: 419-423.
Deshpande, S.S., 1992. Food legumes in human nutrition: A personal perspective. Crit. Rev. Food Sci. Nutr., 32: 333-363.
Ige, M.M., A.O. Ogunsua and O.L. Okon, 1984. Functional properties of the proteins of some Nigerian oilseeds: Conophor seeds and three varieties of melon seeds. J. Agric. Food Chem., 32: 822-825.
Amadou, I., M.T. Kamara, A. Tidjani, M.B.K. Foh and L. Guo-Wei, 2010. Physicochemical and nutritional analysis of fermented soybean protein meal by Lactobacillus plantarum Lp6. World J. Diary Food Sci., 5: 114-118.
Ogunbusola, M.E., T.N. Fagbemi and O.F. Osundahunsi, 2010. Amino acid composition of Lagenaria siceraria seed flour and protein fractions. J. Food Sci. Technol., 47: 656-661.
Ogundele, J.O. and A.A. Oshodi, 2010. Proximate composition and some functional properties of three varieties of Lagenaria siceraria melon seeds. Res. J. Agric. Biol. Sci., 6: 108-112.
Ogundele, J.O., A.A. Oshodi and I.A. Amoo, 2012. Comparative study of amino acid and proximate composition of Citurllus colocynthis and Citrullus vulgaris seeds. Pak. J. Nutr., 11: 247-251.
Ogundele, J.O., A.A. Oshodi, C.O. Fakunle, A.O. Oyebanji and I.A. Amoo, 2013. Evaluation and comparison of the amino acid composition of three varieties of Lagerena siceraria melon seed flours. Food Sci. Qual. Manage., 15: 36-42.
Ogundele, J.O., A.A. Oshodi, T.A. Sanni and I.A. Amoo, 2013. Protein isolates of gourd melon seeds and their functional properties. Am. J. Food. Nutr., 3: 176-181.
Oloyede, O., 1984. Taxonomy of West African Flowering Plants. Longman Group Ltd., London, UK.
Oshodi, A.A., 1992. Proximate composition, nutritionally valuable minerals and functional properties of Adenopus breviflorus benth seed flour and protein concentrate. Food Chem., 45: 79-83.
Oshodi, A.A. and M.O.A. Adeladun, 1993. Proximate composition, some nutritionally valuable minerals and functional properties of three varieties of Lima bean (Phaseolus lunatus Linn.) flour. Int. J. Food Sci. Nutr., 43: 181-186.
Oshodi, A.A., K.O. Ipinmoroti and E.I. Adeyeye, 1997. Functional properties of some varieties of African yam bean (Sphenostylis stenocarpa) flour-III. Int. J. Food Sci. Nutr., 48: 243-250.
Pearson, D., 1981. Chemical Analysis of Foods. 7th Edn., J&A Churchill, London, UK.
Sanchez-Vioque, R., A. Clemente, J. Vioque, J. Bautista and F. Millan, 1999. Protein isolates from chickpea (Cicer arietinum L.): Chemical composition, functional properties and protein characterization. J. Food Chem., 64: 237-243.
Timbo, B., K.M. Koehler, C. Wolyniak and K.C. Klontz, 2004. Sulfites-A food and drug administration review of recalls and reported adverse events. J. Food Protect., 67: 1806-1811.
Wayne, 1996. The wild and wonderful world of gourd. W.P. Armstrong, Vol. 5, No. 3.
Downloads
Published
Issue
Section
License
Copyright (c) 2015 Asian Network for Scientific Information

This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.