Research Article Open Access

Extraction and Isolation Phytochemical and Antimicrobial Activity of Limonoid Compounds from Orange Waste Juice

Authors

CORRESPONDING AUTHOR
Ucop Haroen
Department of Agriculture,Agriculture, Faculty of Animal Husbandry, Jambi University, Jambi 36361, Indonesia
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Yetti Marlida
Department of Agriculture, Faculty of Animal Husbandry, Andalas University, Padang 25163, Indonesia
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Mirzah
Department of Agriculture, Faculty of Animal Husbandry, Andalas University, Padang 25163, Indonesia
|
Agus Budianyah
Department of Agriculture,Agriculture, Faculty of Animal Husbandry, Jambi University, Jambi 36361, Indonesia
Published on: July 15, 2013
DOI 10.3923/pjn.2013.730.735
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Abstract

Antibacterial activity limonoid compounds of three different solvent extracts (methanol, ethyl acetate and n-heksane) prepared by soxhlet extractor from orange waste juice were screened against two pathogenic bacteria Escherichia coli and Salmonella enteridis. The highest antibacterial potentiality was exhibited by the limonoid-ethyl acetate followed by the (limonoid-methanol) and (limonoid-n-hexane).The orange waste juice extract can be considered to be as equally potent as the antibiotics. The zone of inhibition, Minimum Inhibitory Concentration (MIC) of the orange waste juice extracts showed that limonoids extracted with ethyl acetate (limonoids-ethyl acetate) resulted in better inhibition of limonoids extracted with (limonoids-methanol) and (limonoids-n-hexane). The concentration 250 ppm of MIC were test showed inhibition of (limonoids-ethyl acetate) higher with a percentage minimum (44.15 for the bacterium Escherichia coli and 14.69 for the salmonella enteridis) than (limonoids-methanol) (10.67 and 3.84) and (limonoids-n-hexane) (35.58 and 14.10). In vitro antibacterial screening of crude (limonoid-ethyl acetate) (limonoid-methanol) and (limonoid-n-hexane) of methanol extract of orange juice waste with concentration 250 ppm were test showed the clear zone in millimeters (11.75 for the bacterium Escherichia coli and 10.25 for the salmonella enteridis) than limonoid-methanol (8.00 and 9.00) and limonoid-n-hexane (8.00 and 9.25). However, when compared to synthetic antibiotics (neomiditril and coleridin), the clear zone of limonoids lower (24%) but still showed his ability as an anti-microbial. (Limonoids-ethyl acetate) dose of 250 ppm showed better activity than doses of (limonoid-methanol) and (limonoid-n-heksana) for the bacterium Escherichia coli to salmonella enteridis bacterial at 11.918 and 9.158. From these results we can conclude (limonoids-ethyl acetate) extract better than (limonoids-methanol) and (limonoids-n-hexane) and can be used as a substitute for anti biotic synthetic antimicrobial. The phytochemical analysis of orange waste juice extracts showed presence of triter penes, alkaloid, flavonoids, steroid, phenolic, saponin.

Keywords:

Antimicrobial activity, limonoid compounds, MIC, orange juice waste, phytochemical

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Haroen, U., Marlida, Y., Mirzah, & Budianyah, A. (2013). Extraction and Isolation Phytochemical and Antimicrobial Activity of Limonoid Compounds from Orange Waste Juice. Pakistan Journal of Nutrition, 12(8), 730–735. https://doi.org/10.3923/pjn.2013.730.735
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