Research Article Open Access

Effects of Calcium (Ca), Phosphorus (P) and Manganese (Mn) Supplementation during Oil Palm Frond Fermentation by Phanerochaete chrysosporium on Rumen Fluid Characteristics and Microbial Protein Synthesis

Authors

Novirman Jamarun
Faculty of Animal Science, Andalas University, Padang, Indonesia
|
Mardiati Zain
Faculty of Animal Science, Andalas University, Padang, Indonesia
|
Arief
Faculty of Animal Science, Andalas University, Padang, Indonesia
|
CORRESPONDING AUTHOR
Roni Pazla
Faculty of Animal Science, Andalas University, Padang, Indonesia
Published on: May 15, 2017
DOI 10.3923/pjn.2017.393.399
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Abstract

Objective: The aim of this study was to evaluate the effects of calcium (Ca), phosphorus (P) and manganese (Mn) supplementation during Oil Palm Frond (OPF) fermentation by Phanerochaete chrysosporium on rumen fluid characteristics and Microbial Protein Synthesis (MPS). Materials and Methods: This study was conducted using a randomized block design with 3 treatments (the addition of Ca, P and Mn) and 5 replications. The following treatments were performed: T1 = 2000 ppm Ca+1000 ppm P+150 ppm Mn, T2 = 2000 ppm Ca+1500 ppm P+150 ppm Mn and T3 = 2000 ppm Ca+2000 ppm P+150 ppm Mn. The data were analyzed using one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test for comparisons among the treatment means. The parameters measured were as follows: pH, Volatile Fatty Acids (VFAs) (mM), NH3 (mM), total protozoa (cells mL–1) (log 10), total bacteria (cells mL–1) (log 10) and MPS (mg mL–1). Results: The results revealed that VFA levels were significantly increased by the addition of P in T3. However, T1, T2 and T3 non-significantly affected (p>0.05) pH, NH3, total protozoa, total bacterial and MPS. Conclusion: The supplementation of OPFs with 2000 ppm Ca, 2000 ppm P and 150 ppm Mn resulted in the best rumen fluid characteristics and MPS.

Keywords:

Calcium, manganese, MPS, oil palm frond, Phanerochaete chrysosporium, phosphorus

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Jamarun, N., Zain, M., Arief, & Pazla, R. (2017). Effects of Calcium (Ca), Phosphorus (P) and Manganese (Mn) Supplementation during Oil Palm Frond Fermentation by Phanerochaete chrysosporium on Rumen Fluid Characteristics and Microbial Protein Synthesis. Pakistan Journal of Nutrition, 16(6), 393–399. https://doi.org/10.3923/pjn.2017.393.399
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References

1.
Directorate General of Estate Crops, 2015. Tree crop estate statistic of Indonesia 2014-2016. Ministry of Agriculture, Jakarta, Indonesia.
2.
Devendra, C., 1990. Roughage resources for feeding in the ASEAN region. Proceedings of the the 1st ASEAN Workshop on Technology of Animal Feed Production Utility Food Waste Material, (TAFPUFWM'90), Bandung, Indonesia.
3.
Febrina, D., N. Jamarun, M. Zain, Khasrad and R. Mariani, 2014. Biological delignification by Phanerochaete chrysosporium with addition of mineral Mn and its effect on nutrient content of oil palm frond. Proceedings of the 16th AAAP Animal Science Congress, November 10-14, 2014, Yogyakarta, Indonesia, pp: 1723-1726.
4.
Zain, M., J. Rahman and Khasrad, 2014. Effect of palm oil by products on in vitro fermentation and nutrient digestibility. Anim. Nutr. Feed Technol., 14: 175-181.
5.
Singh, D. and S. Chen, 2008. The white-rot fungus Phanerochaete chrysosporium: Conditions for the production of lignin-degrading enzymes. Applied Microbiol. Biotechnol., 81: 399-417.
6.
Suparjo, 2010. Improving nutritive value of cocoa pod husk as feedstuff by bioporcesses with Phanerochaete chrysosporium with Mn2+ dan Ca2+. Ph.D. Thesis, Bogor Agricultural Institute, Bogor.
7.
Febrina, D., N. Jamarun, M. Zain and Khasrad, 2016. Effects of calcium (Ca) and manganese (Mn) supplementation during oil palm frond fermentation by Phanerochaete chrysosporium on in vitro digestibility and rumen fluid characteristics. Pak. J. Nutr., 15: 352-358.
8.
Zain, M., N. Jamarun and Zulkarnaini, 2010. Effect of phosphorus and sulphur supplementation in growing beef cattle diet based on rice straw ammoniated. Asian J. Scient. Res., 3: 184-188.
9.
Zain, M., N. Jamarun and A.S. Tjakradidjaja, 2010. Phosphorus supplementation of ammoniated rice straw on rumen fermentability, synthesised microbial protein and degradability in vitro. World Acad. Sci. Eng. Technol., 4: 357-359.
10.
Febrina, D., N. Jamarun, M. Zain and Khasrad, 2016. The effects of P, S and Mg supplementation of oil palm fronds fermented by Phanerochaete chrysosporium on rumen fluid characteristics and microbial protein synthesis. Pak. J. Nutr., 15: 299-304.
11.
Tyler, H.D. and M.E. Ensminger, 2006. Dairy Cattle Science. 14th Edn., Pearson Prentice Hall, New Jersey.
12.
Brook, E.J., W.R. Stanton and A. Wall-Bridge, 1969. Fermentation methods for protein enrichment of cassava. Biotechnol. Bioeng., 11: 1271-1284.
13.
Ogimoto, K. and S. Imai, 1981. Atlas of Rumen Microbiology. Japan Scientific Societies Press, Tokyo, ISBN: 9784762202643, Pages: 231.
14.
Plummer, D.T., 1971. An Introduction to Practical Biochemistry. 1st Edn., MacGraw Hill Publishers, London.
15.
Church, D.C., 1988. The Ruminant Animal Digestive Physiology and Nutrition. 2nd Edn., O and B Books, Inc., New York, USA.
16.
Fathul, F. and S. Wajizah, 2009. The addition of Mn and Cu mikromineral rations toward biofermentation activity in vitro rumen of sheep. Livest. Vet. Sci. J., 15: 9-15.
17.
Mukhtarudin and Liman, 2006. Determination of the level of use of organic minerals to improve bioprocess rumen of sheep in vitro. J. Anim. Husb. Sci. Indonesia, 8: 132-140.
18.
McDonald, P., R.A. Edwards, J.F.D. Greenhalgh and C.A. Morgan, 2010. Animal Nutrition. 7th Edn., John Willey and Sons, New York, USA.
19.
Erwanto, T.S., D. Sastradipradja and M.A. Nur, 1993. Effects of ammoniated zeolit in metabolic parameters of rumen microbes. Indonesian J. Trop. Agric., 1: 5-12.
20.
Waldron, M.R., F.N. Schrick, J.D. Quigley, J.L. Klotz, A.M. Saxton and R.N. Heitmann, 2002. Volatile fatty acid metabolism by epithelial cells isolated from different areas of the ewe rumen. J. Anim Sci., 80: 270-278.
21.
Sakinah, D., 2005. Studies mineral probiotic supplementation on production VFA, NH3 and digestibility substances in sheep food. Undergraduate Thesis, Bogor Agricultural Institute, Bogor.
22.
Arief, 2013. Supplementation of probiotics in dairy goats rations based byproducts of palm oil processing industry. Ph.D. Thesis, Andalas University, Padang.
23.
Uhi, H.T., A. Parakkasi and B. Haryanto, 2006. The effect of supplementation on the catalytic characteristics and sheep rumen microbial population. Media Peternakan, 29: 20-26.
24.
Suryapratama, W. and F.M. Suhartati, 2012. Increasing rumen microbial protein synthesis with additional dietary substrate of Saccharomyces cerevisiae and soybean oil. Anim. Prod., 14: 155-159.
25.
Stewart, C.S., 1991. The Rumen Bacteria. In: Rumen Microbial Metabolism and Ruminant Digestion, Jouany, J.P. (Ed.). INRA, Paris, France, pp: 15-26.
26.
Hindratiningrum, N., M. Bata and S.A. Santosa, 2011. Products rumen fermentation and microbial protein production of local cattle fed hay ammoniation and some feed ingredient sources of energy. Agripet, 11: 29-34.

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